安祖花组织培养和植株再生的研究

1,2,1,1,1*(1.,610066;2.,610031)[][],,[],(92%),82%1/2MS+6-BA1.0mg/L+KT0.1mg/L1/2MS+IBA0.5mg/LMS+IBA0.5mg/L,11(VV),30d,91%[],5560d,;;;Q943.1A0517-6611(2008)06-02238-02StudyontheTissueCultureandPlantRegenerationofAnthuriumandraeanumLind.GEFang-lanetal(CollegeofLifeScience,SichuanNormalUniversity,Chengdu,Sichuan610066)Abstract[Objective]Theresearchaimedtoprovidebasisforlarge-scaleproductionofAnthuriumandraeanumLind.seedlings.[Method]Withleafblade,petioleandaerialrootintissuecultureseedlingasexplants,thecallusinductionofA.andraeanumwascarriedoutandtheoptimumapproachforthelarge-scaleproductionoftheregeneratedplantsinA.andraeanumwerediscussed.[Result]Leafblade,petioleandaerialrootoftissuecultureseedlingcouldallsuccessfullyinducetheproductionofcallus,amongwhichthestimulaterateofleafbladewashighest(92%)andthatoftheaerialrootoftissuecultureseedlingcouldreach82%.Theoptimummediumforinducingtheadventitiousbuddifferentiationfromcalluswas1/2MS+6-BA1.0mg/L+KT0.1mg/L.Bothmedium1/2MS+IBA0.5mg/LandmediumMS+IBA0.5mg/Lcouldinductionrootproductionfromtheadventitiousbudswithoutobviousdifferences.Whenthemixtureofperliteandflowerfoam(bythevolumeratioof11)wastakenastheculturesubstrateforthetrans-plantationoftissuecultureseedlings,thesurvivalrateofA.andraeanumseedlingsreached91%.[Conclusion]Withtheaerialrootsasexplants,theplantregenerationinalargescaleonlyneeded5560dandtheproductioncostwasalsogreatlydecreased.KeywordsAnthuriumandraeanumLind.;Callus;Buddifferentiation;Plantregeneration(2004-6)(1970-),,,,,*2007-10-23(AnthuriumandraeanumLind.),,[1]1974,[2-5],,,,[3-7],,,11.1,,1.21.2.1,30min,0.01%5min80%30s0.1%5min,3,,1.52.0cm,2.0cm,1.2.24:1/2MS+2.4-D1.0mg/L+6-BA4.0mg/L;1/2MS+2.4-D1.0mg/L+KT0.1mg/L;MS+KT2.0mg/L;1/2MS+6-BA1.0mg/L,pH5.8,6.0g/L36,26,15002000lx,12h/d1.2.3,3,:1/2MS+6-BA1.0mg/L+KT0.1mg/L;1/2MS+NAA0.2mg/L+6-BA2.0mg/L;1/2MS+NAA0.2mg/L+6-BA1.0mg/L1.2.21.2.41.0cm,:1/2MS+0.5mg/LIBA;MS+0.5mg/LIBA1.2.51/2MS22.1[2,4-6],415d,,40d,,,35d,60d14,,,;,,V,(1ab),,,,,,,,,,,,,JournalofAnhuiAgri.Sci.2008,36(6):2238-2239,224281%(1)1Table1Theeffectofdifferentinductionmediaoncallusfromleaf,petioleandaerialrootNo.formediumPetioleNo.ofinvestigatedStatistic%InductionrateLeafNo.ofinvestigatedStatistic%InductionrateAerialrootNo.ofinvestigatedStatistic%Inductionrate2116765752922117811510675446851911571763548224523001752956173121002.2,0.7cm2,3(2)3,(1cd),,,,(2),342Table2TheeffectofdifferentmediaonbudinductionNo.MediaCallusNo.ofbudsBudlengthBuddiameterResultsofcomprehensiveevaluation1/2MS+6-BA1.0mg/L+KT0.1mg/LAMoreLongThick1/2MS+NAA0.2mg/L+6-BA2.0mg/LBLessLongRelativelythick1/2MS+NAA0.2mg/L+6-BA1.0mg/LCMoreShortThin:ABCNote:A,B,Cindicatetheindexofgood,commonandweak,respectively.2.3,(1e),7,12,1.02.0cm2:a;b;c;d;e;fNote:aindicatescallusinducedfromleaves;bindicatescallus;cindicatescallusdifferentiation;dindicatesadventitiousbuds;eindicatesplantlets;findicatestransplantedregeneratedseedlings.1Fig.1RapidpropagationofAnthuriumandraeanum(2242)9322366,,VB1,L3VB1MS100,,VB1[15],,4,L30.1mg/L2,4-D1.5mg/L6-BAIAAKT,[14]IAA0.1mg/L,0.1mg/L,IAA,IAA0.1mg/L2,4-D6-BA,(18mg/L),2,4-D,,,4mg/L2.4-D[8],2,4-D,0.1mg/L2,4-D,2,4-D,2,4-D,[16],2,4-D,,,,,2,4-D2,4-D,,,,2,4-D[14],6-BA2mg/L,2mg/L,,1mg/L1.5mg/L,,6-BA1.5mg/L,,2,4-D,,[1]MAESOC,CHIBBARRN,CASWELLK,etal.Somaticembryogenesisfromisolatedscutellaofwheat:effectsofphysical,physiologicalandgeneticfactors[J].PlantSci,1996,121:75-84.[2]SHARMAVK,RHAOA,VARSHNEYA,etal.ComparisionofdevelopmentalstageofinflorescenceforhighfrequencyplantregenerationinTriticumaestivumL.andT.durumDesf[J].PlantCellRep,1995,15:227-231.[3]RAJYALAKSMIAG,MAHESHWARIAK,MAHESHWARISC.Highfrequen-cyregenerationofplantsfromtheleaf-basesviasomaticembryogenesisandcomparisonofpolypeptideprofilesfrommorphogeneniccalliinwheat(Triticumaestivum)[J].PlantPhysiol,1991,82:617-623.[4]VIERTELK,HESSD.Shoottipsasanalternativesourceforgenerableembryo-geniccallusculture[J].PlantTissueCellandOrganCult,1996,44:183-188.[5],.[J].,1992,(11):850-855.[6],.[J].,1991,(4):267-272.[7].[J].,1993(1):55-61.[8]OZGENM,TURETM,ALTINOKS,etal.Efficientcallusinductionandplantregenerationfrommatureembryocultureofwinterwheatgenotypes[J].PlantCellRep,1998,18:331-335.[9]WARDKA,JORDANMC.Callusformationandplantregenerationfromimma-tureandmatureembryosofrye[J].ProQuestAgricultureJournals,2001,37:361-368.[10],,.[J].,2002,11(4):46-48.[11],,.[J].,1990,5(1):22-27.[12]MURASHIGET,SKOOGFA.Arevisedmediumforrapidgrowthandbioassayswithtobaccotissures[J].Plantphysiol,1962,15:473-494.[13]BECKERD,LORZH.Productionoffertiletransgenicwheatbymicroprojectilebombadment[J].PlantTissueCellandOrganCulture,1994,5:299-307.[14],,,.[J].,2003,23(9):1565-1570.[15],,,.VB1[J].,2003,18(4):19-22.[15],,,.2,4-D,KT[J].,2004,22(3):203-205.(2239)2.4,7d,,,[11(VV)],2028,,75%90%,35d110h30d,91%,1f3,,92%;,;,82%,,,,,,5560d,,[1].[J].,2001(1):48-51.[2]PIERIKRLM,STEEGMANSHHM,VANDERMYSJAJ.PlantletformationincallustissuesAnthuriumandraeanumLind[J].ScientiaHorticulturae,1974(2):193-198.[3],,,.[J].,2003,23(6):1006-1009.[4],,.[J].,2004,31(1):69-72.[5].TDZCPPU[J].,2004,