羟基自由基的产生与测定

X((),,510640):(HO),,,,,,HO(77K,)23ESR;HO;:;;;,,(HOO-),,(HO)(O-2)(HOO),,,Mn2+Cu2+Fe2+,[1,2]:H2O2+Mn+M(n+1)++HO+HO-H2O2+M(n+1)+Mn++HOO+H+HOO-+M(n+1)+Mn++O-2+H+(M),,,,,,[1,2,3]:H2O2+HO-[HOO-+O2OH2O2+HOO-HO+O-2+H2OH2O2+HOO-HO+HOO+HO-H2O22HO(130)(100,)HOO-HO,,[3],F2,2.32eV,,HO,HOO-2HOO[4]HO,,,Fenton,:H2O2+Fe2+HO+HO-+Fe3+,,,,,,54PaperScience&Technology2003Vol.22No.6X20276023,,1ESR(ESR,ElectronSpinReso2nance)(EPR,ElectronParam2agneticResonance),,,(SpinTrapping),,ESR,,%,,ESRDMPO(5,5--1--1-,5,5-dimethyl-1-pyrroline-1-oxide)HOO-2[5],77KESR,3ESR,ESRESR,,,,ESR,2(HPLC,HighPerformanceLiquidChromatography),,,HPLC2.1HPLC(DMSO),DMSO,HODMSOHO(CH3SO2H),,HPLC,[6]:(CH3)2SO+HOCH3SO2H+CH3(DMSO)()CH3SO2HArNNxArN=NSOOCH3()Ar,X,,,HOFukuiGC(FastYellowGCSalt)HO,-,HPLCFentonHO[6]HPLC::Capcell-ParkNH2;:150mm4.6mmI.D.;:;:0.8000(3:100,v/v);:1mL/min;:ShimadzuSPD-10AV-,C-R6A;:285nm;:20LFukuiHPLC1,[6]1HPLCmax(nm)(cm)GCFastYellowGCSalt2858.97TRFastRedTRSalt31010.51BBFastBlueBBSalt4305.00ALFastRedALSalt3303.34,GC,Fe2+2.2HPLC()HO[7]:642003226COOHOH+HOCOOHOHOH+HOCOOHOH+COOHOH()(2,3-,49%)(2,5-,40%)(11%)2,3-2,5-HPLCFentonHO,HPLC2,3-2,5-Fe2+H2O2[7]HPLCHO3ESRHPLC,HOHO,HO,HPLCHO,DMSOFentonHO,BB,:(3:1),420nm,,[8]FentonHO,2,3-,,510nm,Fe2+H2O2,HO,[9]HO,,,,HO4Fenton,(Ce3+),280nm360nm,Ce3+HOCe4+,,360nm,Ce3+:Ce3++HO+H+Ce4++H2O,,,[10]Fenton,,,3,5--1,4-,:+HO+CH2O+NH33,5--1,4-3,5--1,4-,,[11],,ESR,HO,;,,,[1]Carlton,W.Dence,Douglas,W.Reeve.PulpBleaching2PrinciplesandPractice.TappiPress,Atlanta,Georgia,USA,1996,p349350,p415416[2]H-mChang,J.F.Kadla,H.Jameel.TheChemistryofLigninModelCompoundReactionswithPeroxyOxidants.Proceedingsof10thInternationalSymposiumonWoodandPulpingChemistry(ISWPC),1999,Yokohama,Japan,p1219(75)74PaperScience&Technology2003Vol.22No.6tonitesystemwasusedasretentionanddrainageaidsforbleachedwheatstrawpulp.Theeffectofdosagesofcationicpolyacrylamideandmodifiedbentonitewerediscussed.ThemechanismofretentionanddrainagewerediscussedthroughthemeasurementofZetapotentialandtheobservationofscanningelectronmicroscopy.ResultsshowedthatHydrocolmicroparticlesystemproducingmicro2flocculationcouldimprovethedrainabilityofpulp,fillerretentionandbetterpaperstrengthproperty.Keywords:cationicpolyacrylamide;modifiedbentonite;microparticlesystem;retentionanddrainageaids(47)[3]MonicaEK,JosefGierer,KerstinJansbo.StudyontheSelectivityofBleachingwithOxygen2ContainingSpecies.Holzforschung,1989,(43)6:391396[4]JosefGierer.TheReactionsofLigninswithOxygen2ContainingSpecies.Proceedingsof7thISWPC,1993,Beijing,p301307[5],,..,1993,20(4):326327[6]S.Fukui,Y.Hanasaki,S.Ogawa.High-PerformanceLiquidChromatographicDeterminationofMethanesulphinicAcidasaMethodfortheDeterminationofHydroxylRadicals.J.Chro2matography,1993,630:187193[7],..,1996,14(5):405407[8],,.Fenton.,1999,26(1):6769[9],,.Fenton,.1996,23(2):184186[10],,.Fenton.,1998,26(12):14601463[11],..,2002,32(3):288292TheGenerationandDeterminationofHydroxylRadicalsWangShiliangZhangZengHuangGanqiang(StateKeyLaboratoryofPulp&PaperEngineering,SouthChinaUniversityofTechnology,Guangzhou,510640)Abstract:Hydroxylradicals(HO)showstrongabilityofreactiononligninandcarbohydrate.Intheprocessofpulpperoxidebleachingthehydroxylradicalsaregeneratedduetotheexistenceoftransitionmetalionsorthein2creasingofreactiontemperatureinthesystem.Thissituationmakesimportanteffectonbleachingresults.Moreattentionhasbeenpaidontheresearchofmethodsforthedeterminationofhydroxylradicalsinthefieldofmedicine,biochemistryandenvironmentalchemistry.Somemethodswerepresentedinrecentyears.Inthisar2ticleseveralmethodsforthedeterminationofhydroxylradicalsareintroducedaccordingtosomereferences,in2cludingtheSpinTrapping-ElectronSpinResonancemethodwithpreservingthesampleunderverylowtemper2ature(77K,liquefiednitrogen)for23days,themethodsofhigh-performanceliquidchromatographic(HPLC)andspectrophotometrybyusingdimethylsulphoxideandsalicylicacidasthetrappingagensforhy2droxylradicals.Theelectrochemicalmethodisalsomentioned.Keywords:hydroxylradical;determination;peroxidebleaching;HPLC57PaperScience&Technology2003Vol.22No.6