硕士论文-超声骨刀特定刀头对组织刺激的对比观察(1)

泸州医学院硕士学位论文超声骨刀特定刀头对组织刺激的对比观察姓名：黄力申请学位级别：硕士专业：@指导教师：郑光勇20070101120KHz195320HortonPiezosurgery[1][2]2102006620071010%“+”“++”3“+++”10“+++”“++”“+”“+”?2P0.01P0.054ContrastingObservationofTheTissuesthatwereStimulatedbytheSpecifiedBurofPiezosurgeryAbstract:Objective:Theultrasonicwasthesonicwavethatthefrequencyexceeded20kilo-hertz.Ithadcloselyrelationshipwiththemedicine.Fromtheultrasonicdiagnosistechniqueoriginally,totheultrasoniclithotripter,theultrasonicdentalscaler,theultrasoniccataractemulsifier,theultrasonictumorextractorandsoon,whichapplicatedextensivelyatpresent.Wecouldsay:theadvanceoftheultrasonictechniqueacceleratedtheprogressofthemedicineinsomedegree.FollowingthegraduallydevelopmentoftheresearchandtheprogressiveattentionthatpayonthetheprinciplesofMinimallyInvasiveSurgical,theapplicationoftheultrasonicextendedanddevelopedfurtherintheregionofthemedicine.Followingbeingapplicatedinthetherapyoftheparadentosisandpulposis,therewasthefirstreportinthe1953thattheultrasonictechniquewasapplicatedintheincisionofthehardtissuesoftheoral,andbefore20yearsapproximately,Hortonandhiscompanionsapplicatedtheultrasonictechniquetoincisethebone.TheultrasonicoperationinstrumentthatwasusedbroadlynowadayswasthePpiezosurgery,itcouldbeusedintheregionofthealveolussurgery,5implantsurgery,neurosurgery,Spinalsurgery,orthopedicsurgeryandsoon.It’sprinciplewastoutilizeaspecialtechniquethatcouldconcentratethehigh-stregthultra-sonic,switchtheelectricenergytothemechanicalenergyusingthespecialswitchingequipment,throughhighfrequencyultra-sonicoscillationinthespecialextent,maketheintrinsicwaterofthetissuesandcellsthattheinstrumenttouchedbevapouredandthehydrogenbondoftheproteinbesplit,sodestroythebonetissuesthatneedbedissectedthoroughly[1].TheburofthePiezosurgerycouldtouchthebonetissueshomogeneously,incisetheboneaccuratelyandstablelyandtakethebonetissueandpowderawayfromtheoperationregionsrapidly.ThespecialworkfrequencyofthePiezosurgerymadeithavegoodabilitytodistinguishsoftorhardtissues[2].Comparedwiththetraditionaloperationinstruments,thePiezosurgerycouldprotectthesofttissues,suchasbloodvessels,nerves,mucosasandsoon,better.Nowadays,therewasfewreportabouttheuseofthePiezosureryintheourcountry,mostreportscamefromEuropeandAmerica.Thesereportsweretheintroductionsofthefunctionorcasesreportonthewhole.Therewasnotacomparativeresearchreportthatwasthetissuepathologicalchangeaboutthesoftand(or)hardtissuesbeingincisedbythePiezosurgeryandthetraditionaloperationinstruments.ThestudywouldusethePiezosurgeryandthetraditionaloperationinstrumentstoincisethesoftandhardtissuesinvivounderdifferentdegrees,andcontrasttheirdamagedegreeaboutthetissuesthroughobservingthechange6differencesofthepathologicalslices,theresultsmaybethetheoryfoundationaboutthePiezosurgerywouldbepopularizedinthemedicine.Methods:Toselect10patientswhovisitedoralandmaxillofacialwordoftheMianYangStomatologicalHospitalfrom2006\6to2007\1randomly.Accordingtothepatient’sconditionandremedyscheme,thedoctorsmadetheoperationonallpatientsandincisedsomesoftand(or)hardtissues.Ontheoperation,whileincisingsometissues(thesetissueswerenecessaryforthestudy),protectingsometissuesascontrolteamusingthespecialinstrumentsfirstly;thenusingthespecialbursofthePiezosurgerytoincisethesoftand(or)hardtissuesexperiencingdifferenttimeinvivointhelightofstudyproject;fortheremaindersamesofttissues,usingthestimulationofthehaemostatandelectricalsurgeryunit;fortheremaindersamehardtissue,usingthetraditionalboneoperationinstrumentstoinciseexperiencingdifferenttimes.Whentheincisionoperationwascompleted,thestudytissuewasconservedinthesterilizedvesselpreparedbeforehandthatcontainedthe10%formaldehydefixationfluid.whenallthetissueswereconservedinvessels,thevesselsweresenttothedepartmentofpathologyoftheMianYangTCMHospitalandwereembeddedbyparaffin,slicedandH-Estainedimmediately.Finallythesliceswereobservedunderthemicroscopeinordertodiscernthechangedegreesofthetissueswhichwereincisedbydifferentinstruments.Whenusingthemicroscopetoobservethetissues,thecontrolteamshouldbeobservedfirstly,ifthe7sliceofthecontrolteamhasbeeninvadedbythetumorcell,theslicesofthisteamwereabandoned.Results:Accordingtothechangedegreesofthetissuesunderthemicroscopethatwerestimulatedbydifferentinstruments,thedamagedegreesweredividedintothreegraderoughly:mildchange:themarkofthisgroupwas“+”.Theappearancewas:mildcongestion,dropsy,apomorphosisofthetissues,mildhypochromasiaofthecaryon,elongationofthecaryoninthelengthwisemilddistortion,mildorganizationeffectoftheelectriccurrent.mediumchange:themarkofthisgroupwas“++”.Theappearancewas:cellengorgement,tissuehemaleucindeposition,abnormalcurvatureorbreakage,partialdenaturationandnecrosis,hypochromasiaofthecaryonobviously,theshapeofthecaryonlookingasbarrierorturbulence,chromatinolysis,theorganizationeffectoftheelectriccurrentobviously.seriouschangethemarkofthisgroupwas“+++”.Theappearancewas:theconstructionofthetissuesdissolvinganddisappearancecompletely,thechangelookingascharring,nuclearfragmentation,chromatinolysis,collagenfibersengorgeme