课时跟踪检测(十五)细胞的分化、衰老、凋亡和癌变

1()ABmRNACDNADBRNADNA2(2016)()ARNABCDARNA3()ABCDD4()ABBmRNACDDBB5(2016)()ABCDD6kB()()AkBBkBCkBDkBCkBkBkBkBkB7Tpel()ABTpelmRNACDB8(2016)()abABaCbDAb9()ABCDB10.(a)(b)(c)()AabBbCbDcDababbbc11c-Myc()Klf4()Sox2Oct-3/4Oct-3/4Oct-3/4Sox2c-MycKlf4iPS()(1)Oct-3/4_____________________________________________________________________________________________________________________(2)iPSiPS________(3)DNADNA________5-________(1)Oct-3/4c-MycKlf4c-MycKlf4Klf4Fas(2)iPS(3)DNARNA(1)c-MycKlf4c-MycKlf4Fas(2)(3)()RNA12(2016)132G(1)________(2)________________DNA___________________________________________________(3)1________________________________(4)2____________C________________________________________________________________________(5)GGTPGTPATPATPGTP____________(1)(2)(3)1()()(4)2AB(mRNA)mRNAC(5)ATPAATPGTPGGTP(1)(2)()(3)(4)(5)13()()ABCDD14(2016)C()()()ABCC10hD1011hBCC10h1011h15RPMI-1640(HDE)(K562)1SurvivinBcl-22(1)K562________(2)1HDE________hK562________(3)HDEK562SurvivinBcl-2mRNA__________cDNA________cDNA2______________-Actin________2HDE2K562SurvivinBcl-2____________(4)HDE_____________________________________________________K562(5)__________mL/L(1)K562(2)1FL1/FL2HDE48hFL1/FL2ATP(3)K562mRNAcDNAPCRDNARPMI-1640HDEHDERPMI-1640RPMI-1640-Actin2HDE2K562SurvivinBcl-2HDE2K562SurvivinBcl-2(4)12HDESurvivinBcl-2(K562)(5)112.8mL/L(1)(Survivin)(2)48ATP(3)PCRHDERPMI-1640(4)(5)12.8