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ForTechnicalServiceCallYourLocalBio-RadOfficeorintheU.S.Call1-800-4BIORAD(1-800-424-6723)GenePulser®IIElectroporationSystemInstructionManualCatalogNumbers165-2105,165-2106,165-2107,165-2108,165-2109,165-2110LifeScienceGroup2000AlfredNobelDriveHercules,California94547Telephone(510)741-1000Fax:(510)741-5800www.bio-rad.comAustralia,Bio-RadLaboratoriesPty.Ltd.,BlockY,Unit1,RegentsParkIndustrialEstate,391ParkRoad,RegentsPark,NSW2143Phone0299142800•Fax0299142889Austria,Bio-RadLaboratoriesGes.m.b.H.,Auhofstraße78D,A-1130Wien•Phone(01)-8778901•Fax(01)-8765629Belgium,Bio-RadLaboratoriesS.A.-N.V.,Begoniastraat5,B-9810Nazareth•Phone09-3855511•FreePhone0800/97032•Fax09-3856554Brazil,Bio-RadLaboratories(Brazil),RuadosInvalidos212-5andar,Lapa-RiodeJaneiro-RJ,CEP20331-020•Phone55215076191Canada,Bio-RadLaboratories(Canada)Ltd.,5671McAdamRoad,Mississauga,OntarioL4Z1N9•Phone(905)712-2771•Fax(905)712-2990China,Bio-RadChina(Beijing),Rm615,ShangFangPlaza,No.27,NorthThirdRoundCenterRoad,WestDistrict,Beijing100029Phone86-10-8201-1366/68•Fax86-10-8201-1367Denmark,Bio-RadLaboratories,Generatorvej8C,2730Herlev•Phone454452-1000•Fax454452-1001Finland,Bio-RadLaboratories,Pihatörmä1A,FIN-02240Espoo•Phone358(0)98042200•Fax358(0)98041110France,Bio-RadLaboratories,3,BoulevardRaymondPoincaré,92430Marnes-la-Coquette•Phone0147956965•Fax0147419133Germany,Bio-RadLaboratoriesGmbH,Heidemannstraße164,D-80939München,Postfach450133,D-80901MünchenPhone08931884-177•Fax08931884-123HongKong,Bio-RadPacificLtd.,Unit1111,11/F,NewKowloonPlaza,38TaiKokTsuiRoad,TaiKokTsui,KowloonPhone852-2789-3300•Fax852-2789-1257India,Bio-RadLaboratories(India)Pvt.Ltd.,B&B1,EnkayTowersVanijyanikunj,UdhyogViharPhaseV,Gurgaon,Haryana122016Phone(91-124)-6398112/113/114•Fax(91-124)-6398115Israel,Bio-RadLaboratories,Ltd.,14HomaStreet,P.O.Box5044,RishonLeZion75150•Phone039514124•Fax039514129Italy,Bio-RadLaboratoriesS.r.l.,ViaM.Peroglio23,00144Rome•Phone34915905200•Fax34915905211Japan,NipponBio-RadLaboratoriesKK,7-18Higashi-Nippori5-chome,Arakawa-kuTokyo116-0014•Phone03-5811-6270•Fax03-5811-6272Korea,Bio-RadKoreaLtd.,CambridgeBuilding,1461-15Seocho-DongSeocho-Ku,Seoul137-070•Phone82-2-3473-4460•Fax82-2-3472-7003LatinAmerica,Bio-RadLatinAmerica,14100PalmettoFrontageRoad,Suite101,MiamiLakes,FloridaUSA33016•Phone305-894-5950•Fax305-894-5960Mexico,Bio-RadLaboratoriosMexico,AdolfoPrietoNo.1653,Col.DeValle,CP.03100,MexicoD.F.•Phone5255342552to54•Fax5255245971TheNetherlands,Bio-RadLaboratoriesB.V.,Fokkerstraat10,3905KVVeenendaal•Phone0318-540666•Fax0318-542216NewZealand,Bio-RadLaboratoriesPtyLtd.,POBox300-571,Albany,Auckland•Phone64-9-4152280•Fax64-9-4433097Norway,Bio-RadLaboratories,JohanScharffenbergsvei91,N-0694Oslo•Phone47-23-38-41-30•Fax47-23-38-41-39Russia,Bio-RadLaboratorii,ul.Butirskaya79B,office156RF-125015Moscow•Phone70959799800•Fax70959799856Singapore,Bio-RadLaboratories,Singapore,211HendersonRd.#03-02,HendersonIndustrialPark,159552•Phone65-2729877•Fax65-2734835Spain,Bio-RadLaboratories,S.A.,LopezdeHoyos,245-247,28043Madrid•Phone34-91-590-5200•Fax34-91-590-5211Sweden,Bio-RadLaboratoriesAB,Vintergatan1,Box1097,S-17222Sundbyberg•Phone46(0)8-55512700•Fax46(0)8-55512780Switzerland,Bio-RadLaboratoriesAG,Nenzlingerweg2,CH-4153Reinach•Phone061-717-9555•Fax061-717-9550UnitedKingdom,Bio-RadLaboratoriesLtd.,Bio-RadHouse,MaylandsAvenue,HemelHempstead,HertfordshireHP27TDPhone01813282000•FreePhone0800-181134•Fax01442-25911800-0000000Sig1200Bulletin0000US/EGRevABio-RadLaboratoriesM1652105RevCWarrantyTheGenePulserIIelectroporationsystemiswarrantedagainstdefectsinmaterialsandworkmanshipfor1year.Ifanydefectsoccurintheinstrumentsoraccessoriesduringthiswarrantyperiod,Bio-RadLaboratorieswillrepairorreplacethedefectivepartsatitsdiscretionwithoutcharge.Thefollowingdefects,however,arespecificallyexcluded:1.Defectscausedbyimproperoperation.2.RepairormodificationdonebyanyoneotherthanBio-RadLaboratoriesoranauthorizedagent.3.Damagecausedbysubstitutingalternativeparts.4.UseoffittingsorsparepartssuppliedbyanyoneotherthanBio-RadLaboratories.5.Damagecausedbyaccidentormisuse.6.Damagecausedbydisaster.7.Corrosioncausedbyimpropersolventorsample.Thiswarrantydoesnotapplytopartslistedbelow:FusesForanyinquiryorrequestforrepairservice,contactBio-RadLaboratories.InformBio-Radofthemodelandserialnumberofyourinstrument.IMPORTANT:ThisBio-RadinstrumentisdesignedandcertifiedtomeetIEC1010-1*safetystandards.Certifiedproductsaresafetousewhenoperatedinaccordancewiththeinstructionmanual.Thisinstrumentshouldnotbemodifiedoralteredinanyway.Alterationofthisinstrumentwill:Voidthemanufacturer’swarrantyVoidtheIEC1010-1safetycertificationCreateapotentialsafetyhazardBio-RadLaboratoriesisnotresponsibleforanyinjuryordamagecausedbytheuseofthisinstrumentforpurposesotherthanthoseforwhichitisintended,orbymodificationsoftheinstrumentnotperformedbyBio-RadLaboratoriesoranauthorizedagent.*IEC1010-1isaninternationallyacceptedelectricalsafetystandardforlaboratoryinstruments.TableofContentsPageSection1Introduction.....................................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