利用双向导入系解析水稻抽穗期和株高QTL及其与环境互作表达的遗传背景

作物学报ACTAAGRONOMICASINICA2009,35(8):1386−1394:[e-mail](948)(2006-G512006-G1)*(Correspondingauthor):,E-mail:[e-mail]():2009-01-21;Accepted():2009-03-13.DOI:10.3724/SP.J.1006.2009.01386利用双向导入系解析水稻抽穗期和株高QTL及其与环境互作表达的遗传背景效应王韵1,2程立锐2孙勇2周政2朱苓华2徐正进1徐建龙2,*黎志康2,31/,110161;2/,100081;3InternationalRiceResearchInstitute,DAPOBox7777,MetroManila,Philippines:Lemont,QTL,QTLQTL1617,5QTL(QHd2QHd8aQPh3QPh5QPh12),QTL3QTL(QHd8aQHd9QHd10b),QHd8a2~3d,2~3d,QTL7,QTL,QHd3QHd8aQPh3QPh4,QTL,:;;;;;GeneticBackgroundEffectonQTLExpressionofHeadingDateandPlantHeightandTheirInteractionwithEnvironmentinReciprocalIntrogressionLinesofRiceWANGYun1,2,CHENGLi-Rui2,SUNYong2,ZHOUZheng2,ZHULing-Hua2,XUZheng-Jin1,XUJian-Long2,*,andLIZhi-Kang2,31ShenyangAgriculturalUniversity/KeyLaboratoryofCropPhysiology,Ecology,GeneticsandBreeding,MinistryofAgriculture,Shenyang110161,China;2InstituteofCropSciences/NationalKeyFacilityforCropGeneResourcesandGeneticImprovement,ChineseAcademyofAgriculturalSciences,Beijing100081,China;3InternationalRiceResearchInstitute,DAPOBox7777,MetroManila,PhilippinesAbstract:Expressionofquantitativetraitisaffectedbygeneticbackgroundandenvironment.GeneticbackgroundeffectonQTLmappingandQTLbyenvironmentinteractionforheadingdate(HD)andplantheight(PH)inBeijingandHainanenvironmentsweredissectedusingalargesetofreciprocalintrogressionlines(ILs)derivedfromajaponicavariety“Lemont”andindicavariety“Teqing”.ThetwosetsofILsshowedtransgressivesegregationforthetwotraits.Total16and17main-effectQTLswereidentifiedforHDandPHinthetwoenvironments,respectively.Amongthem,onlyfivemain-effectQTLs(QHd2,QHd8a,QPh3,QPh5,andQPh12)weredetectedunderthetwobackgrounds,indicatingexpressionofmostmain-effectQTLsarespecifictogeneticbackground.Threemain-effectQTLs(QHd8a,QHd9,andQHd10b)byenvironmentinteractionsforHDweresignifi-cantlydetectedunderthetwobackgrounds,ofwhichthatofQHd8ahadearlierheadingfor2–3daysinHainan,butdelayedheadingfor2–3daysinBeijing.Therefore,QHd8acouldbeconsideredasanimportantmain-effectQTLforHD.BycomparisonwiththeQTLmappingresultspreviouslyidentifiedinthesevendifferentmappingpopulationsderivedfromthesameparentsin8:QTL1387differentenvironments,somestablyexpressedmain-effectQTLsincludingQHd3,QHd8a,QPh3,andQPh4wereidentifiedun-derdifferentbackgroundsandenvironments,suggestingtheseQTLscouldbeusedinmarker-assistedbreedingforHDandPH.Onthebasisofthemappinginformation,marker-assistedimprovementofHDforaricevarietyunderdifferentenvironmentswasdeeplydiscussed.Keywords:Quantitativetraitloci;Geneticbackground;Gene×environmentinteraction;Reciprocalintrogressionlines;Headingdate;Plantheight[1-2]DH[3-4]F2[5][6-7]QTL,QTLQTL[8-10],[11-12],QTLQTLQTL,QTLQTL,TanksleyNelson[13]QTL(AB-QTL),,QTL[14-15][11,16][17-18][19-20]QTL,[21-25]QTL,QTL,Lemont,,QTL,QTL1材料与方法1.1LemontF1,F1,LemontBC1F1,2~4,201Lemont(LT-ILs)(30BC2F5131BC3F240BC4F3)252(TQ-ILs)(133BC2F596BC3F423BC4F3),1.220061120075,,,2,12,25cm×20cm,2,(headingdate,HD),8(plantheight,PH)1.3151SSR3gl-l()C()Ph(),,133BC2F5Lemont131BC3F2,MapManagerQTX18[26]Lemont1661cM1559.1cM,11.7cM10.9cMLemont,93,;,57,29,28Lemont1.4QTLSASPROCGLM[27]QTL,P0.001QTL1QTL,F[19]2结果与分析2.1Lemont89.1cm138835102.3cm,90.2cm95.8cm,Lemont;106d99d99d105.5d,Lemont,LT-ILLemont83.8%,23.7%~99.4%,TQ-IL88.9%,60.7%~100.0%,11.1%~16.2%(1)(2),,13cm,,;,,6d,,图1双向导入系中特青基因组所占比例的分布Fig.1DistributionofTeqing-genomecompositionofthetworeciprocalintrogressionlines图2双向导入系在北京和海南的株高和抽穗期分布Fig.2FrequencydistributionsofplantheightandheadingdateofthetwointrogressionlinesinBeijingandHainanenvironments2.2QTL,16QTL(13)Lemont10QTL,,0.85~4.27d6QTL,3QTL(QHd2QHd8aQHd12b)Lemont,3QTL(QHd3QHd6bQHd10b)Lemont,1.09~4.43d2QTL(QHd2QHd8a),2QTL,8:QTL138916QTL,3(QHd8aQHd9QHd10b)(2),,,QHd8a,2QTL图3双向导入系在北京和海南检测到影响抽穗期和株高的主效QTL及其在染色体上的分布Fig.3Linkagemapsandchromosomallocationsofmain-effectQTLsaffectingplantheight(PH)andheadingdate(HD)detectedinthetworeciprocalintrogressionlines(ILs)underthetwodiverseenvironments(HainanandBeijing)2.3QTL,17QTL(13)Lemont10QTL,9(QPh7),QPh12,97QTL,QPh3QPh12,QPh12,6LemontQPh3QPh5QPh123QTL,Lemont,17QTL,QTL,,139035表1双向导入系在北京和海南环境下影响抽穗期(HD)及株高(PH)的主效QTLTable1Main-effectQTLsaffectingheadingdate(HD)andplantheight(PH)detectedinBeijingandHainaninthereciprocalin-trogressionlinesBeijingHainanGeneticbackgroundTraitQTLChr.1)MarkerintervalFF-value2)AdditiveeffectFF-valueAdditiveeffect3)Population4)TypeLemontHDQHd22RM29–RM32420.04−2.7242LemontHDQHd44RM303–RM25513.19−1.802,3,72LemontHDQHd55RM13–RM28914.97−2.65LemontHDQHd6a6RM30–RM34014.86−1.873,62LemontHDQHd8a8RM72–RM33965.16−4.271,2,4,5,71LemontHDQHd8b8RM149–RM4479.14−0.8532LemontHDQHd99RM316–RM21935.57−3.191,71LemontHDQHd10a10RM467–RM27113.13−1.366,72LemontHDQHd1111RM224–RM1239.81−1.132,31LemontHDQHd12a12RM4A–RM1926.50−2.98TeqingHDQHd22RM423–RM2925.473.1742TeqingHDQHd33RM81B–RM23113.9−1.921,2,4,5,71TeqingHDQHd6b6RM3–RM27511.52−1.092,3,62TeqingHDQHd8a8RM72–RM33954.954.431,2,4,5,71TeqingHDQHd10b10RM228–RM14713.13−1.622,72TeqingHDQHd12b12RM19–RM24711.333