环境内分泌干扰物研究的进展

2009TransactionsofOceanologyandLimnology3:100326482(2009)03200912073,3(,266003):100,(Vitellogenin,VTG),VTG:;(VTG);:X171.5:A(VTG),,,,,VTG,,VTG,VTG,VTG,(PCBs)(NP)2100VTG,,VTG,,VTGVTG1VTGVTG,,(DES)Angus[1],(Gambusiaaf2finis)172(EE2),1.0gEE2g21,VTG,EE2Peters[2],100ngL21EE221d(Fundulusheteroclitus)VTG,2Heis2ka[3],(80%2)VTG,23:(3020021130671618):(19832),,,E2mail:[e-mail]3:(19672),E2mail:[e-mail]:2008204207VTG[4];,2VTGmRNA,VTG[5][6],42(42NP)42(42OP),VTGLv[7],E2A(BPA),VTG,(500gL21)BPA7d(102100gL21)VTGSchoenfuss[8],EPA42NP,14dVTG,,DDTo,p2DDT[9]Garey[10][11],Christiansen[9],(Oncorhynchusmykiss)VTG,2VTGELISAWestern2blot[12]ELISAE2(Carassiusauratus)VTGLv[8](GSI)VTGVTG,Western2blot0.1mgL21,4()()VTGJean[13](CyprinusCarpio)VTGLiu[14],(PFCs)(Mossabicatilapia)VTG,,PFCs,,,,George[15]PCRmRNAPCBs,ELISA,Barucca[16]RT2PCR:(Mullusbar2batus)(Mugilcephalus)(Gobiusniger)(Thunnusthynnus)VTGmRNA,VTGYan[17]PCR,E2(Daniorerio)(Oryziaslatipes)VTGmRNA,,:1gL21,0.1gL21[18,19],(ELISA)VTG,7.8gL21,,2920093VTG3.1,,,,,[20],,,DNA,VTGZrp,,2,DNA,,,[21]3.2,,,E2,(1),[22],DDTPCBs,,,,(ERE),X(RXR)(TR)ERE4VTG4.1(Alkylphenols,APs)E2(1)Ackerman[23],NPVTG(Zrp)Toomey[24],(Octylphenol,OP)(Ameriurusnebulosus)VTG,Robinson[25],(Pomatoschistusminutus,Pallas)VTGmRNA1978Muller,[21]RoutledgeSumpter[26],,4.2(Phyto2oestrogens)Strauss[27]:(isoflavones)(lignans)[28](1),,3931Table1StructuralformulaofenvironmentalendocrinedisruptorsClassChemicalEnglishnameConfigurationSyntheticestrogens172172estradiol,E2Ethynylestradiol,EE2Phyto2oestrogensSoybeanisoflavoneGenisteinAlkylphenols,APsOctylphenol,OPPolychlorinatedbiphenyls,PCBsPCB1042,4,6,2,62Penta2chlorobiphenylHO2PCB1042,4,6,2,62Penta2chloro242BiphenylolPCB1552,4,6,2,62Hexa2chlorobiphenylPesticidesMethoxychlorCypermethrinPhthalateesters,PAEsDibutylPhthalate,DBPDiisobutylPhthalate,DIBPDibutylPhthalate,DEPDiisononylPhthalate,DINP,DINA4920094.3(Syntheticestrogens)1,,E2,,NimrodBenson[29],(Me2stranol,EE3ME)(Ictaluruspunctatus)VTG,(HSI)Allen[30],EE2(PlatichthysFlesus)VTG,,,;[31][32](Cyprinodonvarie2gates)[33][34]VTGFolmar[33],(Diethylstilbestrol,DES)VTG4.4(Polychlorinatedbiphenyls,PCBs)Andersson,PCB104(2,4,6,2,62Pentachlorobiphenyl)HO2PCB104(2,4,6,2,62Pentachloro242Biphenylol)PCB155(2,4,6,2,62Hexa2chlorobiphenyl)HO2PCB104PCB104PCB155(1),HO2PCB1043[6],HO2PCB104E24.5(Phthalateesters,PAEs)(ButylBenzylPhthalate,BBP)(DibutylPhthalate,DBP)E2,Christiansen[9],ELISAWestern2blot,DBPVTGDBP,[35]Harris[36],:BBPDBPDIBPDEPDINP(1),,,,5,VTG,,22,,VTG,VTG,VTG,VTG,Northern2blotVTGmRNA,[1]AngusRA,StankoJ,JenkinsRL,etal.Effectsof172ethynylestradiolonsexualdevelopmentofmalewesternmosquitofish(Gambusiaaffinis)[J].ComparativeBiochemistryandPhysiologyPartC:Toxi2cology&Pharmacology,2005,140(324):3302339.[2]PetersREM,CourtenaySC,CagampanS,etal.Effectsonreproductivepotentialandendocrinestatus593inthemummichog(Fundulusheteroclitus)afterexposureto172ethynylestradiolinashort2termrepro2ductivebioassay[J].AquaticToxicology,2007,85(2):1542166.[3]Christianson2HeiskaI,SmedsP,GranholmN,etal.Endocrinemodulatingactionsofaphytosterolmix2tureanditsoxidationproductsinzebrafish(Daniorerio)[J].ComparativeBiochemistryandPhysiologyPartC:Toxicology&Pharmacology,2007,145(4):5182527.[4]TremblayL,KraakGVD.Useofaseriesofhomologousinvitroandinvivoassaystoevaluatetheendocrinemodulatingactionsof2sitosterolinrainbowtrout[J].AquaticToxicology,1998,43(223):1492162.[5]MellanenP,Pet¾nenT,Lehtim¾kiJ,etal.Wood2DerivedEstrogens:StudiesinVitrowithBreastCancerCellLinesandinVivoinTrout[J].ToxicologyandAppliedPharmacology,1996,136(2):3812388.[6]AnderssonPL,BlomA,JohannissonA,etal.AssessmentofPCBsandHydroxylatedPCBsasPoten2tialXenoestrogens:InVitroStudiesBasedonMCF27CellProliferationandInductionofVitellogenininPrimaryCultureofRainbowTroutHepatocytes[J].ArchivesofEnvironmentalContaminationandTox2icology,1999,37(2):1452150.[7]LuX,ZhouQ,SongM,etal.Vitellogenicresponsesof172estradiolandbisphenol,A.inmaleChi2neseloach(Misgurnusanguillicaudatus)[J].EnvironmentalToxicologyandPharmacology,2007,24(2):1552159.[8]SchoenfussHL,BartellSE,BistodeauTB,etal.Impairmentofthereproductivepotentialofmalefat2headminnowsbyenvironmentallyrelevantexposuresto42nonylphenolf[J].AquaticToxicology,2008,86(1):91298.[9]ChristiansenLB,PedersenKL,PedersenSN,etal.Invivocomparisonofxenoestrogensusingrain2bowtroutvitellogenininductionasascreeningsystem[J].EnvironmentalToxicologyandChemistry,2000,19(7):186721874.[10]GareyJ,MaryS.Estrogenicandantiprogestagenicactivitiesofpyrethroidinsecticides[J].BiochemicalandBiochemicalResearchCommunications,1998,251(3):8552859.[11],.[J].,2007,35(33):10714210715.[12],,IsodaHiroko,.172[J].,2004,28(3):2362240.[13]SmeetsJM,HolsteijnI,GiesyJP,etal.Estrogenicpotenciesofseveralenvironmentalpollutants,asdeterminedbyvitellogenininductioninacarphepatocyteassay[J].ToxicologicalSciences,1999,50:2062213.[14]LiuChun2sheng,DuYong2bing,ZhouBing2sheng.Evaluationofestrogenicactivitiesandmechanismofactionofperfluorinatedchemicalsdeterminedbyvitellogenininductioninprimaryculturedtilapiahepa2tocyte