猪髂骨TEB与APBSC复合修复犬牙周骨缺损的实验

TEBAPBSC3(710004):APBSCTEB:TEB,APBSCAPBSC2TEB,,APBSC2TEBAPBSC2TEB:106:APBSC13APBSC,APBSCTEB,3,TEB45APBSC2TEB:TEB,,APBSC,13,ö,[1],,,,(Naturalderivedscaffoidmaterialsfortissueengineeredboneandosteoblast,TEB)[2],,,(Autologousperipheralbloodstemcell,APBSC),,,,APBSCTEB110252kg5,2APBSC2TEB(Noculturegroups,NCG);1(1Culture1generationgroups,C1G);2(2C2G);3(3C3G);4513(No:20042K162G16)(45C45G)1,2,3,4,51,65,22212,2cm2cm2cm3(TEB)(FDB)30%48h(24h1)38C30min(31)4h24h110C30h24h30min8min4(APBSC)APBSC2TEB35.0Lgökg(No:05206222,ChuGAL,),110ml45620076366,(No:200225212,,),1000römin1015min4,,2,3,42,,5min,,1.0ml110,,TEB,APBSC2TEB5APBSC10ml,4.0105öcm2,37C,5%CO2100%,5,,,,0.25%0.02%EDTA,2.0103öcm21.0ml110,,TEBAPBSC2TEB56APBSC2TEB10mgökg,0.1mlökg,33,,222cm2cm1cm,APBSC2TEB,,11,1,APBSC2TEB,TEB,TEBAPBSC23APBSC,,TEB,56,45,,,,,1,1,,,,;3,,,45APBSC,APBSC,13,4,,APBSC,,,,,[3],,,,,13,1,,APBSC2TEB,TEB,TEBAPBSC23APBSC,,TEB,,,1,1,,,,,;3,,,5562007636645[4],,,,,,,,[5],[1]PiolettiBP,TakeiH,LinT,etal.Bioactiveceramics;theeffectsofcalciumphosphatecementparticlesonosteoblastfunction.Biomaterials,2000;21:1103[2]PittengerMF,MackayAM,JaiswalSC,etal.Mutilinegepotentialofadulthumanmesenchymalstemcells.Science,1999;284(5411):143[3]ChenSL,YangJW.Differentationofhumanbonemarrowosteogenicstemcellinvatro.Inducationoftheosteoblastphenotypebydexemethasone.Endocrinology,1999;134(1):277[4]BonssarLJ,VacantiCA.Tissueengineering:Thefistdecadeandbeyond.Cell(BiochenSuppl)2003;30:297[5],,,..,2000;14(2):115(:2007202201)3(442000)333333333::Magnus,BL2410,5min,3035min:,,(P0.05P0.01);,(P0.05P0.01):,,,Mö@(MagnoliaofficinalisRehdetluils)(MagnoliaofficinalisRehdetwilsvarbilobaRehdetwils)[1],,,,33303(3)333,1,19000.56g;SCXK()200520008,2h,,,(pH6.4)(pH7.0);Ach(pH5.4),;(pH5.6),;(pH6.8),;(pH5.4),65620076366