细菌表面呈现技术研究进展

(100071),,,,,ProgressinbacterialsurfacedisplayGAORongOKai,ZHANGZhaoOShan(InstituteofBiotechnology,Beijing100071,P.R.China)AbstractThebacterialsurfacedisplayhasgottengreatdevelopmentsincethefirstexamplesofheterologoussurfacedisplayonthesurfaceofEscherichiacoliwasdiscribed.NotonlytheGramOnegativebacteriabutalsotheGramOpositivebacteriacansurfacedis2playtheforeignproteins.Andithasbeenemployedasatoolforfundamentalandappliedresearchinmicrobiology,immunology,molecularbiology,vaccinologyandbiotechnology.Keywordssurfacedisplay,GramOnegativebacteria,GramOpositivebacteria,,,[1]LamBOmpAPhoE[24],,,[5],11.1LamBLamBPorin,421,,,,,[6]XOLamB[7],LamB18,9loop,L4(149166)L6(239265)L9(375405)loopLamB[8],153,374C3L9loop,[9]C3pres2386,1.2OmpAOmpA,100000,3258,4loop,L2L3L4,2170(L2)154(L4)[10,11]OmpAM3spA,10100,,[12]Schorr[13]OmpAOmpAOmpA,N233OmpA1233,COmpA234325,,,,SERP44289245007LETTERSINBIOTECHNOLOGYVol.12No.1Feb.2001[1416]1.3PhoEPhoE,porin,PhoE,,[17]330,16,8loop,L2L3L4L5L7L8,3050[1820]1.4,,,500100020,1500027000[21],type1K88CS31Atype4987ppFimH[2230]CS3,82CTP3,72136[3133](ST)CS3,,[34]1.5,,,,,,,270570,NC,,,[3537]pFLITRX[38],,12,1.6,N,,NLpp,,,LppC,(PAL)LppN9,Francisco[39],LppN9OmpA46159,C,50000,,OPALN,Fuchs[40]PALN,N,C,,[41]TraT,IncF,,,C3preOs[42,43]Cornelis[44]opr,,70,Loop,N,C,,,(ASFV)Vp72HBVTGP63,,,1.7IgAIgA,,C169000,N,C30000Iga,,IgA,IgaIgA,Iga,IgaKlauser[45]12000B(CTB)IgA,Iga,CTBIga,1.8,,N,,14ON834,,,,,1.9Inp(iceOnucleationprotein,Inp),,17:,GP120[46,47]OmpC162,[48]LTOBB,,,Loop,,14,[49]2,,,AM6C,,N,spA,spAIgGC,CLPXTGRREL,,LPXTGTOG[50]C,spAC,IgG,,,M6C,[51]M6,(BCG),,,,,Mtb19Mtb39,A(OspA),,BCGOspA,1001000[52],,,CwbA,,,PrtP,[53]33.1,,,,,,,Stover[52],1001000,,,LamB,,,,,,;,;,3.2,,,12,,,,,gIII(35)gVIII(2700);,;,NC,N,gIII;,(FACS),FranciscaFACS,79%[39]3.3,,BSA,,,,,,,27LETTERSINBIOTECHNOLOGYVol.12No.1Feb.2001;,,;,LamB[54]3.4O,,OO50[55],,:,,,,O10000/;,;,,,3.5,,AFc,,SousaLamB,Cd2+10[56]SPAFc,,,,,,,LoopNC,,,:,,,LamBLppOOmpA;,,;,,,,105106/gDNA,,,,,,,1LittleM,FuchsP,BreitlinFetal.Bacterialsurfacepresentationofproteinsandpeptides:analternativetophagetechnology?TiBTech,1993,11(1):32CharbitA,BoulainJC,HofnungM.Probingthetophologyofabacterialmembraneproteinbygeneticinsertionofaforeignepitope:expressionatthecellsurface.EMBOJ,1986,5:30293FreudlR,MaclntyreS,HenningU.CellsurfaceexposureoftheoutermembraneproteinOmpAofEscherichiacoli.JMolBiol,1986,188:4914AgterbergM,AdriaanseH,Tommassen.UseofmembraneproteinPhoEasacarrierforthetransportofaforeignantigenicdeterminanttothecellsurfaceofEscherichiacoliKO12.Gene,1987,59:1455StahlS,UhlenM.Bacterialsurfacedisplay:trendsandprogress.TiBTech,1997,15(3):1856CharbitA,WertsC,MichelVetal.Adsorptionofbacteriophagelamb2daontheLamBproteinofE.colik12:pointmatutionsingeneJoflambdaresponsibleforextendedhostrange.JBacteriol,1994,176:32047SchirmerT,KellerTA,WangYFetal.Stracturalbasisforsugartranslocationthroughmaltoporinchannelsat3.1Aresolution.Science,1995,267:5128CharbitA.Expressionandimmuneresponsetoforeignepitopesinbacte2ria.Perspectivesforlivevaccinedevelopment.BehringInstMitt,1997,98(2):1359WangJ,MichelV,LeclercCetal.ImmunogenicityofviralBOcellepi2topesinsertedintotwosurfaceloopsoftheEscherichiacolik12LamBproteinandexpressedinanattenuatedaroAstrainofSalmonellaty2phimurium.Vaccine,1999,17(1):110VogelH,JahningF.ModelsforthestructureofouterOmembranepro2teinsofEscherichiacoliderivedfromramanspectroscopyandpredictionmethods.JMolBiol,1986,190:19411PistorS,HobomG.ExpressionofviralhemagglutininonthesurfaceofE.coli.KlinWochenschr,1988,66:11012HaddadD,LiljeqvistS,KumarSetal.SurfacedisplaycomparedtoperiplasmicexpressionofamalarialantigeninSalmonellatyphimuriumanditsimplicationsforimmunogenicity.FEMSImmunolMedMicrobiol,1995,12(3O4):17513SchorrJ,KnappB,HundtEetal.Surfaceexpressionofmalarialanti2gensinSalmonellatyphimurium:inductionofserumantibodyresponseuponoralvaccinationofmice.Vaccine,1991,9(7):67514DaughertyPS,OlsenMJ,IversonBLetal.Developmentofanopti2mizedexpressionsystemforthescreeningofantibodylibrariesdisplayedontheEscherichiacolisurface.ProteinEng,1999,12(7)61315RichinsRD,KanevaI,MulchandaniAetal.BiodegradationoforganophosphoruspesticidesbysurfaceOexpressedorganophorushy2drolase.NatBiotechnol,1997,15(10):98416GeorgiouG,StephensDL,StathopoulosCetal.DisplayofbetaOlacta2maseontheEscherichiacolisurface:outermembranephenotypescon2ferredbylppOompAObetaOlactamasefusion.ProteinEng,1996,9(2):23917BenzR,BauerK.Permeationofhydrophilicmoleculesthroughtheoutermembraneofgramnegativebacteria.EurJBiochem,1988,176:118AgterbergM,AdriaanseH,VanBruggenAetal.OuterOmembrane37:PhoEproteinofEscherichiacolikO12asanexposurevector:possibilitiesandlimitations.Gene,1990,88(1):3719AgterbergM,AdriaanseH,LankhofHetal.OutermembranePhoEofEscherichiacoliasacarrierforforeignantigenicdeterminants:immuno2genicityofepitopesoffootOandOmouthdiseasevirus.Vaccine,1990,8(1):8520JanssenR,TommassenJ.PhoEproteinasacarrierforforeignepitopes.InternRevImmunol,1994,11:1321BlancoJ,BlancoM,GarabalJIetal.Enterotoxins,colonizationfactorsandserotypesofenterotoxigenicEscherichiacolifro