双指标优选肠安颗粒中有机酸提取工艺条件（i）

2005Vol17No.5NATURALPRODUCTRESEARCHANDDEVELOPMENT:20040701:20040926*Tel:[phone];Email:[e-mail](I)黄明*(,710016):,L9(34),,3:10(15),1h,2,1h,A2B2C2D2:;;:O658TheOptimizedExtractConditionsfromChangAnGranules()HUANGMing*(ResearchDevelopmentCenterofXianJahooPharmaceuticalCo.,Ltd.,Xian710016,China)Abstract:ToestablishtheoptimizedextractingconditionsoftwokindsofChineseherbmedicinefromChangangranules,amethodwasdevelopedbyhavingbeenexaminedthecontentoftotalorganicacidsandchlorogenicacidforbothtargets.Theorthogonaltest(fourfactorsandthreelevels)wasdesignedtofinishit.Thefourfactorsweretheamountofwater,soakingtime,extractingtimeandextractingtimes.Atlastanoptimizedextracttechnicshavebeenacquiredbyusing10timesofwater,1hofsoakingandtwoextraction1heachtime,thatistosay,A2B2C2D2wasreasonable.Keywords:doubletargets;changangranules;theorthogonaltest!∀!∀,,,,,,,[1][2],,1,,2000HW1();PHS3C();(Agilent1100,);1/10();()(,:110753200212)2,,,3,L9(34)[3]12.166g,1000mL,600mL,,30min,90min500mL,150%6391Table1FactorsandlevelLevel*AmountofwaterA(min)SoaktimeB(min)EextractiontimeCExtractiontimesD18303012106060231290903*()Amountofwateraddingfirsttime2.2,9(),(60)(80#)200mL,2.2.1总有机酸量[4]25mL,HW1,01mol/LNaOHpH70(PHS3C),NaOH2.2.2绿原酸的测定:HPLC(Agilent1100,),C18(250∃46mm,10m),:25#,04%(13:87),10mL/min,327nm,,10002mg50%1mL40g,22100mL,,45%[5],8h,(120)25mL10L,,2000VID382.322L9(34)Table2ResultsofL9(34)orthogonaltestFactor*Amountofwater(min)Soaktime(min)ExtractiontimeExtractiontimes(mL)Totalorganicacids(%)ChlorogenicacidABCD111116.00.7062122213.40.8993133315.60.8354212312.20.963522319.00.867623128.60.8887313211.30.877832138.90.770933217.10.85635.029.523.522.1--%29.831.332.733.3--&27.331.335.936.7--R7.71.812.414.6--2.4402.5462.3642.429--%2.7182.5362.7182.664--&2.5032.5792.5792.568--R0.2780.0430.3540.235--:%&2:(NaOHmL):DCAB,A1B2C3D3,CADB,A2B3C2D23,,B,,A1B2C3D3;,6402005Vol17No5,,A2B2C2D2,,10(15),1h,2,1h:3,,A2B2C2D2,,(01MNaOH)155mL,0935%,,,,,,1XiaoPG().ModernChineseMateriaMedica(),vol2,edit1.Beijing:ChemicalIndustryPublishingHouse,2002.36392StateAdministrationofTCM,EditorialBoardofChineseHerbMedicine().ChineseHerbMedicine().Shanghai:ShanghaiScienceandTechnologyPublishingHouse,1996.195519573NiZZ().MedicalStatistics().Shanghai:ShanghaiScienceandTechnologyPublishingHouse,2001.4773,2732854JilinMedicalUniversity().MedicalBasicChemistry(),Part1.Beijing:PeoplesMedicalPublishingHouse,1982.2262325LiaoGQ().Studyofextractionmethodofselectivehawbyorthogonaltestdesign.JChinMedMat(),2002,25:661662(上接第644页)3MaYL(),TianZK(),GuoGB(),etal.SeparateandidentificationofarbutininleafofVacciniumvitisidaeaL.ChinTraditHerbDrugs(),1995,26:4724734DUAQ(),WangXR(),ZhouZH(),etal.StudiesonthehydrolyticconditionsofginkgoflavonoidsglycosidesinginkgobilbaleavesbyHPLC.AnhuiMedPharmJ(),2001,5:1641655ZhengYQ(),YaoXS().ApplicationofHPLCinflavonoidsanalysis.ChinTraditHerbDrugs(),1994,25:5425456WatsonDG,PittAR.Analysisofflavonoidsintabletsandurinebygaschromatography/massspectrometryandliquidchromatography/massspectrometry.RapidCommunMassSpectrom,1998,12(4):1531567JuXR(),WangHF().FlavonoedsintheextractofGinkgobilbaL.(EGb)wasdetectedbyHPLC.FoodSci(),2001,22(12):66686412005Vol17No5:(I)