红曲色素提取的工艺条件及稳定性研究

:2003207208;:2003210220:,,1968,,1,1,2(1,100080;2,102206):,,70%60:150;,;,,NaClCaCl2,Cu2+Zn2+;pH311:;;;:TS20113:A:100223186(2004)0120068203StudyonExtractionandStabilityofMonascusPigmentLIHao2ran1DUZhu2wei1WUJun2(1StateKeyLaboratoryofBiochemicalEngineering,InstituteofProcessEngineering,ChineseAcademyofSciences,Beijing100080,China;2BeijingAgriculturalCollege,Beijing102206)Abstract:Pigment2containedricewasproducedafterthesolidfermentationbyMonascusanka.ExtractingprocessofMonascuspigmentfromthefermentedricewasstudied,andtheoptimumextractivesolventwas70%alcohol,theoptimumtemperaturewas60.ThestabilityoftheMonascuspigmentsuchastheheatresistance,radio2tolerance,resistancetoacid,alkalianddrug,wasinvestigated.TheresultsindicatedthatMonascuspigmentwasrelativelystableunder150andintheultravioletradiation,butverysensitivetothesunlight.ChemicalshavedifferenteffectsonMonascuspigment,smallamountofNaClandCaCl2hadalmostnoinfluence,butsomeionssuchasCu2+andZn2+hadobviouseffects;MonascuspigmentwasstableinpH311.Keywords:monascusanka;pigment;solidfermentation;stability,,[1]:,,[2],[3],,,[4],,,,,6[5-6],[1],,19120041JOURNALOFBEIJINGAGRICULTURALCOLLEGEVol.19,No.1Jan.,2004,,,,,,,,11111:30,pH610012,13%4%,12d112,1,1mm;2,,2mm;3,,;4,;5,,,12,,,315513m;3,,512m7m;45,57m,2615d11312,,,;4,,,,;8,300400m,,,,,,,,,114,,(140),2,11572122211(1)2,1h,2,400nm234510nm,32,,23270,2h,20041:6923,,;,;70%,;,,70%(2),70%2h,400nm510nm24,,40,;,,;60,,70,60212(1)51h,70%,150,,(2)pH,,pH70%60,2h,pH,26,pHpH,,pH=311,pH12,,56pH(3),Na+Ca2+Cu2+Zn2+,48h,,Na+Ca2+9918%,Cu2+Zn2+97%,3(1),70%,60(2)150,,;,,;,,Na2ClCaCl2,Cu2+Zn2+;pH,pH=311,,:[1]11:,1997[2]LinTF,DemainAL1EffectofnutritionofMonascussp1onformationofredpigments[J]1ApplMicrobiolBiotechnol,1991,36(1):70275[3],,11,2001,16(3):80285[4]TreiberLR,ReamerRA,RooneyCS,etal1OriginofmonacolinLfromAspergillusTerreuscultures[J]1Jantibiot,1989,42(1):30236[5],,,1[J]1,2002,23(10):33235[6]KimuraK,KomagtaD,MurakawaS,etal1Biosynthesisofmonacolins:conversionofmonacolinJtomonacolinK(mevinolin)[J]1JAntibiot,1990,43:1621216237019