金花茶叶酶法提取工艺条件的研究

206200111JournalofWuxiUniversityofLightIndustryVol.20No.6Nov.2001:1009-038X(2001)06-0582-06:2001-06-04;:2001-10-15.:(1975-),,,.1,1,2,3(1.,214036;2.,510642;3.,410128):.:100,0.4%,pH5.05.5,5055,120min.,39.47%7.39%1.84%,25.5%,FeZnSeMnCoMoGeV95%.:;;;:TS272:AStudyingonEnzymaticExtractionProcessConditionofYellowFlowerCamelliaWANGYuan2feng1,WEIXin2lin1,WANGDeng2liang2,LIUZhong2hua3(1.SchoolofFoodScienceandTechnology,SouthernYangtzeUniversity,Wuxi214036,China;2.SouthChinaAgri2culturalUniversity,Guangzhou510642,China;3.HunanAgriculturalUniversity,Changsha410128,China)Abstract:Thisoptimalextractionparametersandtheextractionrateofnutrientcomponentsofyellowflowercamellia(C.Chrysantha(Hu)Tuyuma)werestudiedbyenzymatichydrolysis.Witharatioof1100betweenyellowflowercamelliaandwater,anadditionof0.4%exogenouspapain,pH5.05.5,mostofnutrientcomponentsandmicroelementswereextractedat5055for2hours.Un2dertheaboveconditions,therewere39.47%increaseofextractionrateofsolublesolid,7.39%in2creaseoftotalsolublesugarand1.84%increaseoftheextractionrateofcrudeproteinaminoacidbyenzymatichydrolysis,comparedwithbypurewaterextraction.Thehydrolyticrateofcrudeproteinwasupto25.5%andtheextractionrateofFeZnCuSeMnCoMoGeVwasupto95%respec2tivelyintheoptimalconditions.Keywords:camellia;exogenousenzyme;extractionrate;hydrolysis,C.chrysantha(Hu)Tuyuma,,,,.,,,.,..,;,,.,,.,...,,,,,,,[115].,.11.1,;,,15000U/g,;,SERVA,0.2U/mg,Aspergillusniger;,,16000U/mg,.1.21.2.1.1.2.21):50mL,,1024h.2):.3):(GB8312287).4):(GB8312287).5):AlCl3.1.2.31):pH;2):,pH.22.11.1Tab.1Basicconstituentsofyellowflowercamellialeaves/%6.5419.7820.7412.403.962.070.230.182.250,100min,,,1,2.1Fig.1Theeffectofwateramountontheextractionrateofyellowflowercamellialeaves2Fig.2Theeffectofwateramountonthecontentsofnu2trientcomponentsofyellowflowercamellialeavesextractedliquid3856:1,2,6090,.90,.,90100.,,,,,.2.3,,18%,5%,,,.2.3.1100,,,3,4.3Fig.3Theeffectofcellulaseconcentrationontheex2tractionrateofyellowflowercamllialeaves4Fig.4Theeffectofcelluseconcentrationonthecontentsofnutrientcomponentsofyellowflowercamellialeavesextractedliquid3,4,.,(),,,,,.3,0.2%,.4,0.1%0.5%,3%5%6.6%8.9%5.0%6.3%,0.1%,.,0.1%0.2%.pH5.05.5,4550.2.3.2100,,,5,6.5Fig.5Theeffectofpecinaseconcentrationontheex2tractionrateofyellowflowercamellialeaves5,6,.0.1%0.2%,,0.2%,,.0.2%,28.83%,8.6%1.0%2.4%,,0.2%.48520pH5.06.0,4550.6Fig.6Theeffectofpectinaseconcentrationonthecon2tentsofnutrientcomponentsofyellowflowercamellialeavesextractedliquid2.3.3,.,,,.100,,,7,8.7Fig.7Theeffectofpapainconcentrationontheextrac2tionrateofyellowflowercamellialeaves7,8,,90%,,,,.8Fig.8Theeffectofpapainconcentrationonthecon2tentsofnutrientcomponentsofyellowflowercamellialeavesextractedliquid011%014%,,014%,,014%,39147%,7139%1184%,,014%.pH5105.5,5055.2.3.4,.,.L16(43),,.,,40%,40%,20%,=0.4x+0.4y+0.2z,=(/80.26)100,2.2,16:10,134,7,15,122.CAB,,pH.,.,,pH5.550,.5856:2pHTab.2Theorthogonalextractionexperiment2generalevaluationatdifferentpH,temperatureandenzymecombinationpH123/%x/%y/%z111173.6055.4011.2953.8667212275.9394.8610.3070.3888313380.3445.8110.7952.6266414483.2698.5711.3975.0193521272.6973.6111.9660.9176622156.8547.8710.6744.0255723484.2492.5711.9373.1191824379.3944.4611.8051.9065931377.7732.3712.9446.64581032496.1297.2414.5880.261001133160.0740.859.8842.34531234277.1293.719.8670.30881341484.1895.6711.7374.28931442379.1838.5411.8949.47621543278.0296.128.8471.43891644150.8967.188.1548.8661R1/478.573.458.9R2/471.676.085.0R3/474.674.662.5R4/476.076.694.3R6.83.235.4,50%,70%,,,.3,0.4%,60%,3,,,,,.,0.4%.0.4%,,,.2.43,,1.8,,.4,25.5%,,90.4%,,58.5%55.3%.5,,45%,71%,95%.,.685203Tab.3Comparisonofpurewaterextractedliquidwithenzymolysisliquidforyellowflowercamellialeaves/%/%/%/%/(mg/g)/%21.233.96-2.075.500.1858.4712.251.8253.513.823.604Tab.4Thecontentsandextractionrateofproteinicaminoacidsanddissociativeaminoacidsofenzymolysisliquidofyellowflowercamelliadryleaves/%/%/%/%/%/%(Asp)0.640.57990.4(Ile)0.300.03511.6(Thr)0.29(Leu)0.510.05210.3(Ser)0.310.06420.6(Tyr)0.210.04320.6(Glu)0.630.08914.1(Phe)0.320.06018.9(Gly)0.350.20558.5(Lys)0.350.04412.6(Ala)0.380.04511.9(His)0.11(Cys)0.090.05055.3(Arg)0.290.0113.7(Val)0.440.07617.3(Pro)0.300.0289.3(Met)0.120.05848.45.641.43925.55Tab.5Thecontentsandextractionrateofmicroelementsofdryleavesandenzymolysisofyellowflowercamellia/(mg/kg)/(mg/kg)/%/(mg/kg)/(mg/kg)/%(Ca)125741854.4014.75(Mn)203.6203.5899.99(Mg)22671617.2071.34(Co)2.012.0099.50(Fe)14.7614.6599.25(Mo)0.020.02100.00(Zn)8.5378.4999.45(Ge)3.073.0799.84(Cu)0.950.9296.84(Sr)22.44.4019.64(Se)5.865.8098.98(V)4.003.9999.75(Li)0.9260.4043.2031).0.1%0.2%,0.2%0.4%,4550,45555055,pH5.05.5,5.56.05.05.5.,16.68%33.3%36.8%,2.6%01.3%,7%8.3%8.9%.3,.0.1%0.2%,70%,95%.2)0.4%25.5%,,90.4%,58.5%55.3%,.FeZnCuSeMnCoMoGeV95%,71%.(593)7856::[1],.()[J].,1990,13(1):4547.[2],.[J].,1997,18(9):6164.[3],.C21[J].,1998,17(2):4346.[4],,.[J].,1987,2(4):2528.[5],,.[J].,1988,3(4):2627,43.[6],.[J].,1987,2(5):2325.[7].[J].,1988,3(2):2932.[8],,.[J].,1987,2(5):2526.[9].()[M].:,1983.[10],.[J].(),1992,5(3):8183,92.[11],.[J].(),1993,8(1):7478.