日粮添加富硒益生菌对奶牛硒吸收转化_抗氧化能力与生产性能的影

:20090616:(30671547);(200739):(1976),,,*,Email:[e-mail]吴显实1,2,张克春1,3,卫程武1,杨家军1,冉林武1,黄克和1*(1.,210095;2.,530004;3.,200032):将36头经产荷斯坦奶牛随机分成4组,对照组喂基础日粮,富硒益生菌(SePro)组每头牛添加108.2g/d,益生菌(Pro)组每头牛添加20.8g/d,亚硒酸钠(Na2SeO3)组每头牛添加5mg/d试验期为产前30d至产后3个月于试验0306090120d,采血样测定全血Se含量GPx活性和血浆丙二醛(MDA)含量;于产后2(初乳)306090d,采乳样测定Se含量;产后每个月测定产奶量1次结果显示,SePro组和Na2SeO3组的全血初乳常乳Se含量和GPx活性均显著高于对照组和Pro组(P0.05);SePro组的全血常乳Se含量和GPx活性(30d除外)均显著高于Na2SeO3组(P0.05);SePro组和Na2SeO3组的血浆MDA含量均显著低于对照组和Pro组(P005);SePro组的血浆MDA含量均显著低于Na2SeO3组(P0.05);与对照组相比,SePro组Pro组Na2SeO3组的泌乳期前3个月的平均日产奶量(4%FCM)分别增加了2.96kg/d(P=0.069)1.64kg/d(P0.05)0.69kg/d(P0.05)结果表明,SePro的作用效果优于Pro和Na2SeO3:硒;乳硒含量;抗氧化能力;体细胞数:S816.72:A:10054545(2010)08111104Effectsofsupplementaryseleniumenrichedprobioticsonabsorptionandconversionofselenium,antioxidativecapacityandperformanceindairycowsWUXianshi1,2,ZHANGKechun1,3,WEIChengwu1,YANGJiajun1,RANLinwu1,HUANGKehe1*(1.CollegeofVeterinaryMedicine,NanjingAgriculturalUniversity,Nanjing210095,China;2.CollegeofAnimalScienceandTechnology,GuangxiUniversity,Nanning530004,China;3.InstituteofDairyofShanghai,Shanghai200032,China)Abstract:ThirtysixmultiparousHolsteincowswererandomlydividedintofourgroups.Controlgroupreceivedabasaldiet.Seleniumenrichedprobiotics(SePro)groupreceivedabasaldietplus108.2gofSePro/dpercow.Sodiumselenite(Na2SeO3)groupreceivedabasaldietplus5mgofSe/dpercow.Probiotics(Pro)groupreceivedabasaldietplus20.8gofPro/dpercow.Experimentperiodwasfromd30prepartumto3mopostpartum.Bloodsampleswerecollectedforanalysisofselenium(Se)levelsandGPxactivitiesofwholebloodandplasmamalondialdehyde(MDA)concentrationsond0,30,60,90and120posttreatemnet.MilksampleswerecollectedfordeterminationofSeconcentrationsond2(colostrum),30,60and90postpartum.Milkyieldsweredeterminedoncemonthlyaftercalving.TheSeconcentrationsofwholeblood,colostrumandmilkandGPxactivitiesinSeProandNa2SeO3groupsweresignificantlyhigherthanthoseofcontrolandProgroups(P0.05).TheSeconcentrationsofwholebloodandmilkandGPxactivities(exceptforond30)inSeProgroupweresignificantlyhigherthanthoseofNa2SeO3group(P0.05).TheplasmaMDAconcentrationsofSeProandNa2SeO3groupsweresignificantlylowerthanthoseofcontrolandProgroups(P0.05).TheplasmaMDAconcentrationsofSeProgroupweresignificantlylowerthanthoseofNa2SeO3group(P0.05).Comparedwithcontrolgroup,theaveragesofdailymilkyield(4%FCM)inthefirst3mooflactationincreasedby2.96,1.64and0.69kg/dinSePro,ProandNa2SeO3groups,respectively.TheseresultsindicatethattheaboveeffectsofSeProaregreaterthanthatofNa2SeO3andPro.Keywords:selenium;milkseleniumconcentration;antioxidativecapacity;somaticcellcount*Correspondingauthor,Email:[e-mail]111120108308ChinJVetSciAug.2010Vol.30No.8(Selenium,Se),,[12](Glutathioneperoxidase,GPx),,,[1];(Malondialdehyde,MDA)[3],,MDA,GPxMDA(Probiotics)[4],[56]Se,SeSe(),,Se(,),[7],,Se,,Se,,1材料与方法1.1(Seleniumenrichedprobiotics,SePro)46.2mg/kg,(70.131.07)%(),4,(Candidautilis)(Lactobacillusacidophilus)(Streptococcusthermophilus)(LactobacillusrhamnosusGG),(2.960.24)107cfu/g,(1.540.31)108cfu/g,(Na2SeO3)()1.2()36,,4(9/):;(SePro)+108.2g/dSePro(5mgSe320.27107cfu);+20.8g/d(320.32107cfu);Na2SeO3+5mL/dNa2SeO3(5mgSe)1.37d,131.40306090120d,,,Se[8]GPxMDA(GPxMDA)2()306090d,Se1.5306090d,,3[9]1.6SPSS(Repeatedmeasures)(Pairedsamples)Tx!s,P0.052结果,1,SePro1,Na2SeO3132.1Se306090120d,SeProNa2SeO3SePro(P0.05),ProSe(P0.05);30d,SeProSeNa2SeO3(P0.05),6090120d,SeProSeNa2SeO3(P0.05)(1)2.2Se2()306090d,SeProNa2SeO3SePro(P0.05);ProSe(P005);SeProSeNa2SeO3,(P=0.066),SeProSeNa2SeO3(P0.05)(2)111220108308ChinJVetSciAug.2010Vol.30No.8表1日粮添加SeProPro或Na2SeO3对奶牛全血Se含量的影响g/L/dSeProaProbNa2SeO3c0120.0411.77d118.439.89d124.7610.51d123.568.04d30122.517.05d172.688.53e124.226.37d166.377.79e60115.689.56d177.3011.27e117.999.93d158.0813.32f90117.785.92d181.158.99e118.685.56d162.4113.07f120125.967.36d187.038.26e121.146.29d162.318.13f:aTreatmenttime(P0.05);b,cTreatmenttime(P0.05);,(P0.05)表2日粮添加SeProPro或Na2SeO3对奶牛乳Se含量的影响g/La/dSeProbProcNa2SeO3d2()48.516.85e88.2111.29f50.135.41e75.545.61f3025.353.96g51.669.39h24.094.76g38.865.46i6021.994.22g48.366.09h23.003.95g35.168.95i9024.885.00g57.599.68h23.635.42g37.696.16i:a;b,c,dTreatmenttime(P0.05);,(P0.05)2.3GPx306090120d,SeProNa2SeO3GPxPro(P0.05),ProGPx(P0.05)6090120d,SeProGPxNa2SeO3(P0.05)(3)表3日粮添加SeProPro或Na2SeO3对奶牛全血GPx活性的影响U/mL/dSeProaProbNa2SeO3c0135.3322.78d130.4821.72d138.9325.29d136.1230.45d30133.4625.42d184.4725.41e142.6527.47d176.7331.76e60145.8227.51d193.8229.67f135.0428.76d172.6127.36e90130.7526.45d207.8324.82f136.6829.32d185.2822.64e120135.0622.37d216.5626.91f142.1423.65d188.3025.37e:aTreatmenttime(P0.05);b,cTreatmenttime(P0.05);,(P0.05)2.4MDA306090120d,SeProNa2SeO3MDAPro(P0.05);ProMDA(P0.05);SeProMDANa2SeO3(P0.05)(4)表4日粮添加SeProPro或Na2SeO3对奶牛血浆MDA含量的影响mol/L/dSeProaProbNa2SeO3c04.280.18d4.310.32d4.170.23d4.260.22d304.510.29d2.500.38e4.490.33d3.210.37f604.850.30d2.800.36e4.920.40d3.590.31f904.630.30d2.880.24e4.550.25d3.670.31f1204.480.26d2.410.28e4.240.27d3.350.28f:a,b,cTreatmenttime(P0.05);,(P0.05)2.5306090d,: