HPV的分子生物学特征及其致病机理

HPV(HPV)20120,85HPV:830011,(STD)(HPV)20120,85HPVDNA,,,HPV,,,HPV90%,(CA)HPV[13]1HPVHPVDNA,1949StrausHPV,,4555nmHPV7188kb1.1HPV[1,2]HPV9(ORF),3(URR)(E)4500bp,E1E88,DNAE1DNA,E2,DNAHPV211E1E2E1HPV,E2HPV211E2,21(BPV21)E2E4HPVE5E2,,HPV216E584(aa),BPV2144aaHPV26E5ORFE5b,E5E5,E5E6E7E6,(E62E6APE62E6BP)E6,E7HPV216E6151aa,Zn2+,2Zn(Znfingers)E6BPCa2+E6,,E6E7,HPVE6p53,E7(Rb)HPV,E7E7,HPV216E798aa,11kdaHPV26211E714kda17kdaHPV216HPV26,N10aaHPV216218231,HPV26211HPV216,E7E6(10%)LL1L2,L1,L21.2HPV[4,5]120042111SectionVirologyForeignMedSci,February2004,Vol11,No.1HPV,DNA,HPV,S,3E5E6E7G1S,E7,G1/S,Rb,RbG0G1S,SG1/SG2/MM(),,,,(check2point)p23pRb(CDK)(DKIs)p21p27p16p15,(DNA),,,,,HPVE7Rb,p21,AP21,G1,HPV,HPV26211S,;HPV216218SHPV:1.2.1:,50100/1.2.2:,50100/1.2.3:,,()DNA+++++++-/+-+-HPVUstavStenlundHPV,,23DNASouthern,E1E2,HPV211,E1,E22HPV[15]HPV,,,,,,,220042111SectionVirologyForeignMedSci,February2004,Vol11,No.1,,,,,HPVHPV,G1/S,S,DNA[1]2.1HPV,HPV,CA,,HPVT,TTTT(TCR)(MHC)CD4MHC2,CD8MHC2,/,T,T,THPV,,CA,,,,HPVMHCHPV2E5HPV(IFN22)2(GM2CSF)21(IL21)CA,,IL26IL210,IL22IL212IL215(EGFR)HPV216E6/E7p53Rb,,IFN2MHC2,MHC2(),HPVIFN2IFN,HPVE71737p48,IFN2;E6TyK2,JAK2STAT,IFN,HPVIFNIFNHPVIL21TCR,TIL22IL22(IL22R),IL22IFN2(NK)IL21,,IL21,IL22TIL22RTIL22IL22R,TCAIL2222(sIL22R),66IL22(3040/d)CA(250mg/d)HPV,CA,2.2HPV,20120HPV,[6]HPV26L1,8L126,311,659870817099,,10,03HPV90%HPV,1618313335394551525658596870Nakagawa[6]RT2PCR(CIN)HPV,30/31(97%)31/31(100%)HPVJE6E7HPV258,HPV233(77%),HPV216(64%),HPV216320042111SectionVirologyForeignMedSci,February2004,Vol11,No.13119%60%1012HPV,,Choo[8]17HPV216,15/17(8812%)178(T178G/D15E)E6,11/17(6417%)A442C/E113DE6,63%6E6,8E7,A647GT846C8812%A657GN29S,T846CLuxton[9],HPV216E6CIN,CIN,E6HPV216HPV,2.2.1()HPV2.2.1.1,30%50%,2.2.1.285%HPVDNAHPV211161831333539455152555658596870738283,HPV2161839,,,90%HPV,HPV2161850%70%2.2.1.3HPV,,HPVHPV21657%,SiHaHPV216DNA1/;HelaHPV218DNA20/2.2.2()HPV2.2.2.1HPV,,HPV2123410,,,2.2.2.2(CA),,,,90%HPV2611CAHPV26211:HPVDNA4127105/ml,41621035135108/ml,4712%CA,,,20502.2.2.3CA,30%90%,2/318CA,34HPV261140425354576684HPV26b11MM76162,HPV21618MM43133395253565866687013(HPV216184556)(HPV2313335515258)(HPV2611424344)[10,11][12]PCR138HPV,HPV216E6E7(72%)(37%)119,HPV21817%15%,HPV2163HPV,,,,1954STD,HPVDNAE6E7,,,HPV[13]HPV216,,HPV2161LaiminsLA.Regulationoftranscriptionandreplicationbyhumanpapillomavirus.InMcCanceDJ(ed.).HumanTumorViruses.ASMpress,Washington,1998,2012223.420042111SectionVirologyForeignMedSci,February2004,Vol11,No.12NeadMAandMcCanceDJ.ActivitieroftheTransformingproteitsofhumanpapillomaviruse.InMcCanceDJ(ed.),HumanTumorViruses.ASMpress,Washington,1998,2252251.3..,2003,(3):107.4..,2001,27(3):16321655..,2001,27(5):2962298.6,.HPVHPV26L1.,200259,14.7NakagawaS,Yoshikawa.H,YasugiT,etal.UbiquitouspresenceofE6andE7transcriptsinhumanpapillomaviruspositivecervicalcarcinomasregardlessofitstype.JMedVirol,2000,62(2):2512256.8ChooKB,WangTSandHumanCJ.AnalysisofrelativebindingoffinityofE72pRBofhumanpapillomavirus16clinicalvariantsusingtheyeasttwohybridsystem.JMedVirol,2000,61(3):2982302.9LuxtonJ,MantC,GreenwoodB,etal.HPV16E6oncogenevari2antsinwomenwithcervicalintraepithelialneoplasia.JMedVirol,2000,60(3):3372341.10WangTS,ChouHF,LiuWM,etal.Semiautomatedtypingofhumanpapillomavirusebyrestrictionfragmaentlengthpolymor2phismanalysisoffluoresencelabeledPCRfragments.JMedVirol,1999,59(4):5362540.11ChanPKS,LiWH,ChanMYM,etal.Highprevalenceofhumanpapillomavirustype58inChineseWomanwithcervicalcancerandprecancerouslesions.JMedVirol,1999,59(2):2322238.12LiT,LuZM,ChenKN,etal.Humanpapillomavirustype16isaimportantinfectiousfactorinthehighincidenceofesophagealcan2cerinAnyangareaofChina.Carcinogenesis,2001,22(6):9292934.13Koutsky..,20021212,22.(2002211228;2004201209)HIVHIV,,,:100050,HIV,,,,;,I,,,,,,1HIV(env),(,,gpl202gp41),,gpl20gp41,3gpl202gp41Gpl20CD4,,gpl20,,,,gp41,gp41N2,gp41,520042111SectionVirologyForeignMedSci,February2004,Vol11,No.1