高效液相色谱法测定人参总皂苷的含量

:20060322;:20060920:阮鸣(1979),女(汉族),安徽六安人,现任南京晓庄学院生命科学系助教,硕士学位,主要从事药用真菌发酵产物的化学成分研究和新药的研究开发工作.阮鸣(,210017):建立传统中药材人参的高效液相色谱分析方法采用高效液相色谱法对人参中的人参皂苷Rg1和人参皂苷Re进行含量测定;色谱柱:KromasilC18(4.6mm250mm,5m);流动相:乙腈-水系统线性梯度洗脱;柱温:35;流速:1.0ml/min;检测波长:203nm该方法人参皂苷Rg1与人参皂苷Re的线性范围分别为12.35~0.38g,12.46~0.38g,平均回收率为98.7%(RSD=1.59%)该方法简单可行,结果准确,重复性好,可作为该药材质量的控制方法:人参;人参皂苷Rg1;人参皂苷Re;高效液相色谱法:R284.2:B:10080805(2007)01013401PanaxginsengC.A.Mey.,,[1];,,,[2],[3],,2000,,Rg1Re,1Agilent1100,QuatPump,VWD-,Agilent,Agilent(CanadaCALEDONLABORATORILESLTD.),,Rg1Re(,0703-200221,0754-200217)22.1色谱条件:,C18[KromasilC18(4.6mm250mm,5m)];:A[-(19:81)],10min,35min(10~45min),AA-B[](96.3:3.7),(1);:Rg1Re,203nm,203nm;:35;Re30001t/minAB1010004596.33.72.2对照品溶液的制备60Rg1Re,ARg10.175mgRe0.162mg,2.3供试品溶液的制备(4)1g,,,40m,l3h,,,,50m,l,,(250W,50kHz)30min,,25m,l,5ml,,,,(10000r/min)10min,,2.4标准曲线的制备60Rg112.35mg,10ml,A,Rg1(1235g/ml)617.5,308.75,154.375,77.1875,38.5938g/ml,10,l,,(Y),(g/ml)(X),,:Y=2.3379X-1.501(r=0.9998),:Rg112.35~0.38g;Re:Y=2.7524X+7.9632(r=0.9999),Re12.46~0.38g2.5精密度实验10,l6,,RSD0.97%,0.80%2.6重现性实验6,,10l,,RSD1.45%,2.7稳定性实验,0,2,4,6,8,10h,RSD1.51%,10h2.8加样回收率实验(Rg10.20%Re0.28%)0.5g,6,2,Rg1(0.841mg/ml)1.0,1.2,1.4m,lRe(0.742mg/ml)1.5,1.9,2.3m,l2.3,98.7%,RSD1.59%2.9样品测定31.0g,2.310,l,30.49%,0.53%,0.52%,0.51%3[4],-,Rg1Re,[5],:A[]-B[](19:81),10min,35min(10~45min),A[]-B[](2278),Rg1Re,,,1342007181LISHIZHENMEDICINEANDMATERIAMEDICARESEARCH2007VOL.18NO.1,,A-(1981),B,1~2:[1].,[S].:,2000:6.[2].[J].2003,4(28):1.[3],,.[J].1999,2(16):154.[4],.Rg1[J].2003,14(8):469.[5],,.[J].1996,5(30):307.:20060328;:20060930:邓惠玲(1971),女(汉族),辽宁大连人,现在读大连理工大学硕士研究生,学士学位,主要从事食品生物方面的研究工作.邓惠玲1,2,姜波2,刘玉欣2,安利佳2(1.,116023;2.,116023):研究不同组合的蓝莓菟丝子益智仁提取物对小鼠学习记忆的影响给小鼠灌胃喂饲不同的提取复合物,给药30d,Y型电迷宫测定小鼠的学习记忆能力;测定小鼠脑组织中超氧化物歧化酶(superoxidedismutase,SOD)和谷胱甘肽过氧化物酶(glutathioneperioxidase,GSH-Px)的活性及丙二醛(malondialdehyde,MDA)的含量蓝莓和菟丝子益智仁的复合物使小鼠在Y-迷宫训练中达到学会标准的次数显著减少;显著提高脑组织中SOD和GSH-PX的活性,降低了MDA的含量蓝莓菟丝子和益智仁的复合物能提高小鼠的学习记忆能力:蓝莓;菟丝子;益智仁;学习记忆:R285.5:A:10080805(2007)01013502EffectsofSeveralTraditionalChineseMedicinalHerbalExtractsonLearningandMemoryinmiceDENGHuiling1,2,JIANGBo2,LIUYuxin2,ANLijia2(1.DalianLightIndustrySchool,Dalian116023,China;2.DalianUniversityofScienceandTechnology,Dalian116023,China)Abstract:ObjectiveToinvestigatetheeffectsofBlueberry,CuscutachinensisLam.andAlpiniaoxyphyllaMiq.extractsonlearningandmemoryinmice.MethodsThemicewerefedwiththethreeherbalextractscompoundfor30days,learningandmemoryabilityofmicewasperformedinY-maze.Thelevelsofsuperoxidedismutase(SOD),glutathioneperoxidase(GSH-Px)andthecontentofmalondialdehyde(MDA)inbrainweredetermined.ResultsThecompoundofthreekindsofherbalextractscouldincreaselearningandmemoryabilityofmice,increasetheactivitiesofSODandGSH-PXanddecreaselevelofMDAofbraintissues.ConclusionBlueberry,CuscutachinensisLam.andAlpiniaoxyphyllaMiq.compoundscanimprovelearningandmemoryabilityofmice.Keywords:Blueberry;CuscutachinensisLam.;AlpiniaoxyphyllaMiq.;Learningandmemory[1],VCVAVE,[2],,[3,4],[5],,,B1B2CE,[6][7],[8]PC12,GAP-43[9];[10],PC12,,,11.1材料1.1.1主要药品及试剂;8,,,30min0.4g/m,l4135LISHIZHENMEDICINEANDMATERIAMEDICARESEARCH2007VOL.18NO.12007181