毕业论文稿香椿黄酮的提取工艺研究20150529

学士学位论文（设计）Bachelor’sThesis论文题目香椿叶总黄酮提取工艺研究作者姓名朱文娟学号20040403027所在院系生命科学学院学科专业名称生物技术导师及职称王友如副教授论文答辩时间2008年5月17日编号2015140714研究类型基础研究分类号Q58湖北师范学院学士学位论文（设计）诚信承诺书中文题目：香椿叶总黄的提取工艺研究外文题目：StudyontechniqueofextractionoftotalflavonoidsfromToonasinensisleaves学生姓名朱文娟学生学号20040403027院系专业生物技术学生班级0403学生承诺我承诺在学士学位论文（设计）活动中遵守学校有关规定，恪守学术规范，本人学士学位论文（设计）内容除特别注明和引用外，均为本人观点，不存在剽窃、抄袭他人学术成果，伪造、篡改实验数据的情况。如有违规行为，我愿承担一切责任，接受学校的处理。学生（签名）：年月日指导教师承诺我承诺在指导学生学士学位论文（设计）活动中遵守学校有关规定，恪守学术道德规范，经过本人核查，该生学士学位论文（设计）内容除特别注明和引用外，均为该生本人观点，不存在剽窃、抄袭他人学术成果，伪造、篡改实验数据的现象。指导教师（签名）：年月日目录1前言......................................................................................................................................11.1香椿的简介....................................................................................................................11.2香椿的开发利用前景..................................................................................................11.3黄酮类化合物研究概况..............................................................................................22材料与方法..........................................................................................................................32.1材料................................................................................................................................32.1.1实验材料...........................................................................................................32.1.2仪器设备...........................................................................................................32.1.3试剂及溶液.......................................................................................................32.2实验方法......................................................................................................................42.2.1样品的处理及提取..............................................................................................42.2.2总黄酮的测定......................................................................................................42.2.3总黄酮提取率计算..............................................................................................42.2.4总黄酮提取的单因素试验..................................................................................52.2.5正交试验设计......................................................................................................53结果与分析..........................................................................................................................63.1料液比对黄酮提取率的影响.....................................................................................63.2乙醇体积对黄酮提取率的影响.................................................................................73.3提取温度对黄酮提取率的影响.................................................................................73.4提取时间对黄酮提取率的影响.................................................................................83.5提取工艺的优化.........................................................................................................93.5.1正交优水平下香椿黄酮的提取率....................................................................104实验结果............................................................................................................................105结论与讨论........................................................................................................................116参考文献........................................................................................................................12中英文摘要香椿叶中总黄酮的提取工艺研究易男（指导老师,王友如副教授）（湖北师范学院生命科学学院湖北黄石435002）摘要:香椿（Toonasinensis）为楝科香椿属多年生落叶乔木，因其具有很高的营养价值和药用价值，而具有广阔的发展前景和开发价值。香椿老叶中含有较高含量的黄酮类化合物，因此具有很强的抗氧化活性。本文以香椿叶为原料,利用紫外分光光度法，以芦丁为对照品，NaNO2-A1(NO3)3-NaOH体系为显色剂测定香椿叶中总黄酮的含量，并利用L9(34)正交试验设计，确立香椿叶中总黄酮的最佳工艺条件为:乙醇体积分数60%、料液比为1∶30(g/mL)、浸提温度70℃、提取时间2h,在此条件下香椿叶片总黄酮提取率为7.95%。关键词:香椿叶；总黄酮；提取工艺中图分类号:Q58StudyonTechniqueofExtractionofTotalFlavonoidsfromToonaSinensisLeavesYiNan(Tutor:WangYouru)(CollegeofLifeSciences,HubeiNormalUniversity,Huangshi,Hubei,435002)Abstract:Cedar(Toonasinensis),aperennialdeciduoustrees，iswidelydistributedinChina,hasalonghistoryofcultivation,becauseofitshighnutritionalvalueandmedicinalvalue,andhasbroadprospectsfordevelopmentandthedevelopmentvalue.FlavonoidscontainhighercontentofoldleavesofToonasinensis,soithasstrongantioxidantactivity.TheinToonasinensisleavesasrawmaterial,ultravioletspectrophotometry,usingrutinascontrolsample,NaNO2-Al(NO3)3-NaOHsystemasthechromogenicreagentforthedeterminationofthecontentoftotalflavonoidsinToonasinensisleaves,andtheL9(34)orthogonalexperimentaldesign,establishedtheoptimumtechnologicalconditionsoftotalflavonoidsfromToonasinensisleaves:ethanolconcentration60%,materialliquidratiois1:30(g/ml),leachingtemperatureof70℃,extractiontimeof2h,undertheconditionsofToonasinensisleavesflavonoidsextractionrateof7.95%.Keywords:toonasinensisleaves;totalflavonoid;extractionprocessChineseLibraryClassification(CLC):Q58湖北师范学院生命科学学院2008届学士学位论文（设计）1香椿叶中总黄酮的提取工艺研究朱文娟（指导老师，王友如副教授）（湖北师范学院生命科学学院湖北黄石435002）1前言1.1香椿的简介香椿ToonaSinensis(A．Juss．)Roem，又名椿、椿树、香芽树，系楝科香椿属落叶乔木[1]，《植物名实图考》称红椿[2]。香椿的皮(