昆仑雪菊挥发油抗氧化活性和抑制淀粉酶和糖苷酶活性研究-20140512

塔里木大学毕业论文2014届毕业生毕业论文昆仑雪菊挥发油体外抗氧化活性及对α-淀粉酶和α-糖苷酶抑制作用研究学生姓名林佩艳学号7021210230所属学院生命科学学院专业应用化学班级14—2指导教师曾红塔里木大学教务处制目录前言.............................................................................................................21材料与方法......................................................................................................21.1实验材料.................................................................................................21.1.1供试材料........................................................................................2塔里木大学毕业论文1.1.2实验试剂........................................................................................21.1.3主要设备........................................................................................31.2试验方法.................................................................................................31.2.1挥发油的提取................................................................................31.2.2昆仑雪菊挥发油的密度................................................................41.2.3挥发油的成分检测方法................................................................41.2.4昆仑雪菊挥发油的抗氧化活性研究............................................51.2.5昆仑雪菊挥发油对α-淀粉酶和α-葡萄糖苷酶的抑制作用......62实验结果与分析..............................................................................................72.1昆仑雪菊挥发油的得率.........................................................................72.2昆仑雪菊挥发油的成分分析.................................................................72.3昆仑雪菊挥发油的体外抗氧化分析.....................................................92.3.1昆仑雪菊挥发油DPPH自由基的清除能力................................92.3.2昆仑雪菊挥发油ABTS自由基的清除能力..............................102.3.3昆仑雪菊挥发油羟基自由基的清除能力..................................102.3.4昆仑雪菊挥发油超氧阴离子自由基的清除能力.......................112.4昆仑雪菊挥发油对α-糖苷酶和α-淀粉酶的抑制作用......................122.4.1昆仑雪菊挥发油对α-糖苷酶的抑制能力.................................122.4.2昆仑雪菊挥发油对α-淀粉酶的抑制能力.................................123结论与讨论....................................................................................................133.1结论.......................................................................................................133.2讨论.......................................................................................................14参考文献...........................................................................................................15致谢...........................................................................................................17塔里木大学毕业论文第1页昆仑雪菊挥发油体外抗氧化活性及α-淀粉酶和α-糖苷酶抑制作用研究林佩艳（塔里木大学生命科学学院，新疆阿拉尔843300）（新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室，新疆阿拉尔843300）摘要：【目的】研究新疆昆仑雪菊挥发油的体外抗氧化活性及抑制α-淀粉酶和α-糖苷酶作用。【方法】采用水蒸气蒸馏法提取昆仑雪菊挥发油，用GC-MS法测定昆仑雪菊挥发油的成分和含量。以BHT为阳性对照，昆仑雪菊挥发油对DPPH自由基、ABTS自由基、羟基自由基和超氧阴离子自由基的清除能力以及对α-淀粉酶和ɑ-糖苷酶的抑制作用。【结果】昆仑雪菊挥发油有清除自由基和抑制α－淀粉酶和α－糖苷酶活性的能力，昆仑雪菊挥发油对DPPH自由基，ABTS自由基，羟基自由基，超氧阴离子自由基在一定浓度范围内都有清除作用，其IC50值分别为0.7852±0.04μg/mL，0.0730±0.0214μg/mL，0.8289±0.0184μg/mL，7.9837±0.0082μg/mL；对α-淀粉酶和α-糖苷酶也有很强的抑制作用，浓度和抑制率在浓度范围内呈现正相关关系，其IC50值分别为0.1402±0.0082μg/mL、0.1538±0.0082μg/mL。【结论】昆仑雪菊挥发油有体外抗氧化作用及抑制α-淀粉酶和α-糖苷酶的作用。关键词：昆仑雪菊；挥发油；自由基；α-淀粉酶；α-糖苷酶Kunlunsnowchrysanthemunessentialoilantibacterialantioxidantactivityresearchpeiyanlin(tarimuniversitycollegeoflifesciences,xinjiangaral843300)(xinjiangproductionandconstructioncorpslaboratoryofbiologicalresourcesprotectionoftarimbasin,xinjiangaral843300)Abstract：【objective】tostudythevolatileoilofxinjiangkunlunsnowchrysanthemuminvitroantioxidantactivityandinhibitionofalphaamylaseandalphaglycosidaseaction.【methods】kunlunsnowchrysanthemumvolatileoilbysteamdistillationextraction,kunlunsnowchrysanthemumbyGC-MSmethod,thecompositionandcontentofvolatileoil.Withbutylatedhydroxytolueneasthepositivecontrol,kunlunsnowchrysanthemumvolatileoilofDPPHfreeradical,ABTSfreeradical,hydroxylradicalandsuperoxideanionfreeradicalremovalabilityaswellastothealphaamylaseandɑglycosidicenzymeinhibition.【results】thekunlunsnowchrysanthemumnaphthascavengingfreeradicalsandinhibitingalphaandalphaglycosidicenzymeactivityofamylase,kunlunsnowchrysanthemumvolatileoilofDPPHfreeradical,ABTSfreeradical,hydroxylfreeradicals,superoxideanionfreeradicalshaveclearroleinthecertainconcentrationrange,itsIC50alue塔里木大学毕业论文第2页concentrationwere1.8058±0.04μg/mL,0.336±0.0214μg/mL,1.99±0.0184μg/mL,13.9675±0.0082μg/mL;Toalphaamylaseandalphaglycosidicenzymeinhibition,concentrationandinhibitionratepresentpositivecorrelationintheconcentrationrange.【conclusion】thekunlunsnowchrysanthemumnaphthainvitroantioxidanteffectandinhibitingtheroleofalphaamylaseandalphaglycosidase.Keywords:kunlunsnow;chrysanthemumvolatileoil;freeradicalsof;alphaamylase;alphaglycosidase塔里木大学毕业论文第2页前言昆仑雪菊是具有新疆区域特色的菊屹品种之一。昆仑雪菊又名“血菊”，维吾尔语“古丽恰尔”，是目前新疆唯一与雪莲齐名且具有降血压、降血脂等独特功效的稀有高寒野生植物，具有极高的药用价值[1]。目前，新疆和田市政府组织有关专家对昆仑雪菊进行相关研究，并委托新疆药物研究所对昆仑雪菊进行相关药理学实验。经研究发现，昆仑雪菊确有降血压、降血脂和降血糖的功效。挥发油是具有广泛生物活性的一类重要成分，是古代医疗实践中较早注意到的药物，多具有祛痰、止咳、驱风、健胃、解热、镇痛、抗菌消炎等作用。民间流传其还具有清热解毒、活血化瘀、和胃健脾之功效，当花茶饮用，可治疗燥热烦渴、心慌、胃肠不适、食欲不振、痢疾及疮疖肿毒等症。花茶，《神农本草经》中的记载：“茶叶。味苦寒……久服安心益气……轻身耐老”，“茶味苦，饮之使人益思、少卧、轻身、明目”，以及唐代陆羽的《茶经》和清代李时珍的《