未成熟粒细胞检测的评估

:167328640(2010)0320171205:R446.11:A1,1,2,2,1,1(1.,610041;2.,610041):(IG);SysmexXE22100IG20,;220d,;101IG,;883,;SysmexXE22100766IG,IGCV4.78%5.35%13.06%,IGCV3.82%6.03%14.54%;2CVIG4.38%5.13%;IG4.3%5.1%,15%,,(r)0.916;r0.8903;IGP0.05,;P0.05,,IGIG95%(0,0.04109/L);(0,0.02109/L)IG95%(0,0.5%);(0,0.4%)IG,:;;;:,,1966,,,EvaluationofimmaturegranulocytedeterminationandestablishmentofitsreferencerangeJIANGHong1,ZENGTingting1,LIUYiling2,LBRuixue2,ZUOYongtai1,ZHUANGLifang1.(1.DepartmentofLaboratoryMedicine,WestChinaHospital,SichuanUniversity,SichuanChengdu610041,China;2.LaboratoryDepartment,WestChinaSchoolofMedicine,SichuanUniversity,SichuanChengdu610041,China)Abstract:ObjectiveToinvestigatetheimmaturegranulocyte(IG)countdeterminationbytheautomatedhematologyanalyzerinclinicalapplicationandestablishanormalreferencerangeofIGofourlaboratorybySysmexXE22100automatedhematologyanalyzer.MethodsAclinicalsamplewasdeterminedsuccessivelyfor20timestoensuretheintra2batchprecision;2qualitycontrolsweredeterminedsuccessivelyfor20daystoensuretheinter2batchprecision.TheIGcountsof101specimensweredeterminedbytheSysmexXE22100hematologyanalyzerandflowcytometryrespectively,andtheresultswerecomparedtoevaluatethecorrelation.883wholebloodspecimensweredeterminedandclassifiedbytheSysmexXE22100hematologyanalyzerandmanualmicroscopyrespectively,andtheresultswerecomparedtoevaluatetheconsistency.766healthysubjectsweredeterminedbytheSysmexXE22100automatedhematologyanalyzerfordeterminingtheIGcountandestablishingthereferencerange.ResultsTheintra2batchcoefficientsofvariation(CV)ofabsoluteIGcountsofhigh,mediumandlowprecisionvalueswere4.78%,5.35%and13.06%respectively.Theintra2batchCVsofIG%were3.82%,6.03%and14.54%respectively.Theinter2batchCVsofabsoluteIGcountsofhighandlowvalueswere4.38%and5.13%respectively.Theinter2batchCVsofIG%were4.3%and5.1%respectively.Allofthemwerewithinthetolerancelimit(15%).Thecorrelationcoefficient(r)betweenSysmexXE22100hematologyanalyzerandflowcytometrywas0.916,andthatbetweenSysmexXE22100hematologyanalyzerandmanualmicroscopywas0.8903.Therewasnosignificantdifferencebetweendifferentages(P0.05),butnotbetweenmaleandfemale(P0.05).IGreferencerangeswereestablishedregardlessofage.TheabsoluteIGcountreferencerangeformalewas(0,0.04109/L),andforfemaleitwas(0,0.02109/L).TheIG%referencerangeformalewas(0,0.5%),andforfemaleitwas(0,0.4%).ConclusionsHematologyanalyzercanbe17120103253LaboratoryMedicine,March2010,Vol25.No3.©1994-2011ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(immaturegranulocytes,IG),,[1,2](IG)[3],,,,(WBC),,[2]SysmexXE22100XEProIGMasterIG,IG,IG,,XE22100IG,,SysmexXE22100IG,[4],IG1.SysmexXE22100(Sysmex);BDFACSCanto(BD);Olympus2.IG:Sysmex;IG:CD162(CD162FITC)CD11b2(CD11b2PE)CD452(CD452PerCP),BD1.4061(25),79222.436447(49),6502333.766,383,383,1575(45)1.SysmexXE22100IG,IGXEIGMaster,WBCdiffIGRNADNAIGDNA,,PMT()[5],IG2.IGWBCCD45WBC,(SSC)CD11b,CD11b,CD16CD11b+CD16-,CD11b+CD16+[5]CD11bCD16CD45IGCD162FITCCD11b2PECD452PerCP,CD45SSC,CD162CD11b+IG,WBCIG3.10WBC2,27120103253LaboratoryMedicine,March2010,Vol25.No3.©1994-2011ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(200WBC,400WBC,WBC)SPSS13.0,t,1.(1):3IG20,sCV%1;(2):23e2CHECK,20d,IGsCV%21IGIG(109/L)XsCVIG(%)XsCV1.530.074.788.000.313.820.960.055.354.950.056.030.070.0113.061.670.2414.542IGIG(109/L)XsCVIG(%)XsCV20.8040.0415.1311.1700.5705.1032.3070.1014.3812.2000.5254.302.IG(0.96109/L)(0.03109/L)1,3(i1,i2,i3)3(j1,j2,j3),1.2%,1.XE22100101SysmexXE22100101XE22100t2(r=0.915,1),2t,(P0.05)1XE22100IG2.XE22100883SysmexXE22100,10,21(210%),2(r=0.890,2)2XE22100IGIG766,IG3,IG,IG,3IG(n=766)37120103253LaboratoryMedicine,March2010,Vol25.No3.©1994-2011ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:1530;3145;4660;617550IGIG,(34)P0.05,IGIG3IGIG(n=200)PIG(109/L)IG(%)120.8210.714130.3500.538140.9540.566230.2500.237240.8510.271340.3120.8004IGIG(n=200)PIG(109/L)IG(%)120.4950.412130.9430.867140.8730.654230.4320.310240.4540.225340.9530.7642.IG(45)IG(5),P0.01,IG4766IG(n=383)5766IG(n=383)5IGIG(109/L)IG(%)200--200--Z--6.692-5.593P-0.010.013.,IGIGSysmexXE22100,IG:95%(0,0.04109/L);(0,0.02109/L)IG:95%(0,0.5%);(0,0.4%)SysmexXE22100IG,IGmaster,IG,IG,,,rIG,,13WBC2%,8WBC(),,,SysmexXE22100IG47120103253LaboratoryMedicine,March2010,Vol25.No3.©1994-2011ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[6],SysmexXE22100IG,,IG,IG,IG:[7]IG,IGIG,,,IG,[1]ShigaS,FujimotoH,MoriY,etal.Immaturegranu2locytecountafterlivertransplantation[J].ClinChemLabMed,2002,40(8):7752780.[2]NigroKG,ORiordanM,MolloyEJ,etal.Performanceofanautomatedimmaturegranulocytecountasapredictorofneonatalsepsis[J].AmJClinPathol,2005,123(4):6182624.[3]FringsDP,MontagB,HeydornA,etal.Immaturegranulocytes,