分子生物学试卷(英文)

西南大学课程考核命题教师：教研室或系负责人：主管院长：年月日第1页共5页—————————————密————————————封——————————————线——————————————学号姓名班年级专业学院西南大学生物技术学院《MolecularBiology》课程试题【A】卷2019～2020学年第1学期期末考试考试时间120分钟考核方式开卷笔试学生类别本科人数98适用专业或科类生物技术年级05级题号一二三四五六七八九十合计得分签名阅卷须知：阅卷用红色墨水笔书写，得分用阿拉伯数字写在每小题题号前，用正分表示，不得分则在题号前写0；大题得分登录在对应的分数框内；统一命题的课程应集体阅卷，流水作业；阅卷后要进行复核，发现漏评、漏记或总分统计错误应及时更正；对评定分数或统分记录进行修改时，修改人必须签名。IDefinethemeaningofthesefollowingterms(30points,3ptsperterm)1.meltingtemperature(Tm)2.Cvalueparadox3.exon4.semi-conservationreplication5.cDNAlibrary6.gelshiftassay特别提醒：学生必须遵守课程考核纪律，违规者将受到严肃处理。《MolecularBiology》课程试题【A】卷第2页共5页7.promoter8.openreadingframe(ORF)9.genefamily10.expressedsequencetag(EST)IIFillinblank(10points,1ptsperblank)1.Thesimplestcloningvectoratleastcontains,andorielements.2.ThediagrambelowshowstwoRNAmolecules.MoleculeIisprocessedtogivemoleculeII.Answerthefollowingquestionsaboutthesetwomolecules:Wheredoesthisconversionhappen?____________________________WhattypeofRNAmoleculeismoleculeI?_________________________WhattypeofRNAmoleculeismoleculeII?_________________________Nametheregions/structuresindicatedbythearrows:A=_________________B=_________________C=_________________D=_________________E=_________________西南大学课程考核(试题【A】卷)第3页共5页——————————————密————————————封————————————线——————————————IIISelecttherightanswer(20points,2ptsperquestion;Selectthebestanswerforeachquestion.)1.ThebondbetweenaphosphategroupandtheribosesugargroupsinRNAiscalledwhichofthefollowing?()(A)Phosphodiesterbond(B)Glycosidicbond(C)Peptidebond(D)Amidebond2.WhichenzymedoesnotplayaroleinDNAreplication?()(A)DNApolymerase(B)Helicase(C)Single-strandedbindingprotein(D)Guanyltransferase3.Splicingistheprocessthatdoeswhichofthefollowing?()(A)Removeintronsandconserveexons(B)Removeexonsandconserveintrons(C)RemovemutatedregionsofprimarytranscriptRNA(D)AddmultipleadenosinebasestotheendofaprimaryRNAtranscript4.Theidealspacingbetweenthe-10and-35regionsofaprokaryoticpromoteris:()bp.(A)14(B)15(C)16(D)175.The5'cappingprocesscreateswhattypeoflinkage?()(A)5'-3'(B)3'-5'(C)5'-5'(D)3'-3'6.Codonsthatcodeforthesameaminoacidarecalled:()(A)Synonyms(B)Similar(C)Degenerates(D)Complements7.WhichofthefollowingisnotacharacteristicofatRNAmolecule?()(A)Darm(B)Betaarm(C)Tarm(D)Anti-codonarm8.Whatsignalstheendoftranscription?()(A)Stopcodon(B)Terminator(C)TheendoftheDNAchain(D)RNApolymeraserunsout9.Thestartcodonis:()(A)AUU(B)UAA(C)AUG(D)UAG10.Whichofthefollowingisthesequenceofthe-10regionofprokaryoticpromoters?()(A)TATAAT(B)TTGACA(C)TATA(D)CAAIVAnalyticalquestions(30points,6ptsperquestion)1.Describebrieflytheattenuationofthetrpoperon.《MolecularBiology》课程试题【A】卷第4页共5页2.DescribetheprincipleofSanger’senzymicmethodforDNAsequencing.3.WhatarethebasicfeaturesofDNAdoublehelixdeducedbyWatsonandCrick?4.PleasedrawaschematicstructureofTTPinthefollowingspace.YoumaywritejusttheletterTforthebase.5.OnthefollowingagarosegelwithmarkerDNAasindicated:a)Placeaplus(+)signonthepositivepoleofthegelandaminus(–)signonthenegativepole.b)Drawthebandorbandsthatyouwouldseeifyoudigesteda3kbplasmidwithEcoRIandranittotheleftofthemarker.AssumetheplasmidhasaMCSwiththefollowingrestrictionsites:BamHI,EcoRI,EcoRV,HindIII,andKpnI.西南大学课程考核(试题【A】卷)第5页共5页——————————————密————————————封————————————线——————————————4kb6kb2kb0.5kbVDiscussthefollowingtopic(10points)DescribeyourunderstandingofCentralDogmaindetail.