乙醇的检测

乙醇的檢測乙醇C2H6O46.07Ethanol.EthylalcoholCAS#:[64-17-5].應用：饮料汽车燃料消毒剂有机合成原料溶剂稀释剂萃取剂洗涤剂Spectrum乙醇品種A2176Alcohol,Denatured,190ProofA1038Alcohol,Anhydrous,CompletelyDenaturedA1040ReagentAlcohol,Denatured,Reagent,ACSE1020EthylAlcohol,190Proof,FCCE1028EthylAlcohol,Absolute,200Proof,Reagent,ACSE1029EthylAlcohol,190Proof,Reagent,ACSE1068DehydratedAlcohol,200Proof,USP,EP,BPE1421Alcohol,190Proof,BiotechGradeE1424DehydratedAlcohol,200Proof,BiotechGradeET107DehydratedAlcohol,200Proof,Undenatured,USPET108Alcohol,190Proof,USPHP612ReagentAlcohol,Denatured,ExceedsA.C.S.Specifications,HPLCGradeHS002ReagentAlcohol,Denatured,HistologicalGradeEX0278Alcohol,Denatured,OmniSolv(R)EX0289EthylAlcohol,MultiPharm,200Proof,EP,JP,USPEX0290EthylAlcohol,Pure,190Proof,HPLCGrade*Proof:酒精纯度,一个酒精纯度相当于0.5％的酒精含量.乙醇檢測的應用相對密度法USP841,SpecificGravity.ET107,Dehydratedalcohol,200proof,undenatured,USP蒸餾法USP611,MethodⅠ.中國藥典2005版一部：附錄ⅨM乙醇量測定法Ⅱa,b,c氣相色譜法（GC）USP611,Alcoholdetermination,MethodⅡ.BE115,BelladonnaTinctureACS10th,Ethylalcohol.HP612，ReagentAlcohol,Denatured,ExceedsA.C.S.Specifications,HPLCGradeUSP467,Residualsolvents,class3.中國藥典2010版二部：附錄ⅦE乙醇量測定法中國藥典2005版一部：附錄ⅨM乙醇量測定法Ⅰ相對密度法-USP841,SpecificGravity,methodⅠET107,Dehydratedalcohol,200proof,undenatured,USPAlcoholcontent:≧99.2%,byweight;≧99.5%,byvolume,at15.56℃.原理:25℃下,相同體積乙醇和水的重量比值,折算成15.56℃下乙醇的重量含量或體積含量.方法:使用同一比重瓶，先將先将一定體積的燒開過的蒸餾水放入其中,分別稱量25℃下空瓶和水裝入后的總重量,兩者之差就是25℃下水的重量.同理測量25℃下乙醇的重量.乙醇和水的重量比为25℃下乙醇的比重.通過查找USPreferencetable–Alcoholometrictable中的數據，確定15.56℃下乙醇的體積含量或者重量含量.比重瓶USPreferencetable–Alcoholometrictable12345678percentagespecificgravitypercentagespecificgravitybyvolumnat15.56℃byweightat25℃at15.56℃byvolumnat15.56℃byweightat25℃at15.56℃10.800.99850.99855143.430.92690.932221.590.99700.99705244.370.92480.930232.390.99560.99565345.330.92280.928243.190.99410.99425446.280.92070.926254.000.99270.99285547.250.91850.924164.800.99140.99155648.210.91640.922075.610.99010.99025749.190.91420.919986.420.98880.98905850.170.91200.917797.230.98750.98785951.150.90980.9155108.050.98620.98666052.150.90760.9133118.860.98500.98546153.150.90530.9111129.680.98380.98436254.150.90300.90881310.500.98260.98326355.170.90060.90651411.320.98140.98216456.180.89830.90421512.140.98020.98106557.210.89590.90191612.960.97900.98006658.240.89360.89951713.790.97780.97896759.280.89110.89721814.610.97670.97796860.330.88870.89481915.440.97560.97696961.380.88620.89232016.270.97440.97597062.440.88370.88992117.100.97330.97497163.510.88120.88742217.930.97210.97397264.590.87870.88482318.770.97100.97297365.670.87610.88232419.600.96980.97197466.770.87350.87972520.440.96850.97087567.870.87090.87712621.290.96730.96977668.980.86820.87452722.130.96610.96877770.100.86550.87182822.970.96480.96767871.230.86280.86912923.820.96350.96647972.380.86000.86643024.670.96220.96538073.530.85720.8636蒸餾法-USP611,MethodⅠ中國藥典2005版一部：附錄ⅨM乙醇量測定法Ⅱ適用範圍USP611,MethodⅠ:流浸膏劑,酊劑中國藥典2005版一部：附錄ⅨM乙醇量測定法Ⅱ:流浸膏劑,酊劑,中藥製劑原理根據樣品的不同性質,加入不同物質,進行預處理.蒸餾,獲得乙醇餾出物.測定蒸餾物的相對密度,折算出乙醇的含量.方法(中國藥典2005版一部：附錄ⅨM乙醇量測定法Ⅱ)Alcoholcontent≦30%:稱取20℃下樣品25ml,置於150-200ml蒸餾瓶中,加水約25ml,蒸餾.待餾出物達23ml時,停止蒸餾.加水至刻度,測量乙醇在20℃下的相對密度,計算出乙醇含量;Alcoholcontent﹥30%:稱取20℃下樣品25ml,置於150-200ml蒸餾瓶中,加水約50ml,蒸餾.待餾出物達48ml時,停止蒸餾.加水至刻度,測量乙醇在20℃下的相對密度,計算出乙醇含量;樣品含有揮發性物質,如揮髮油,三氯甲烷,乙醚,樟腦等.可先加入氯化鈉使之飽和,后加入乙醚,使溶液分層,取水層按常規檢測.樣品含有遊離氨或揮發性酸.可先加入稀硫酸或氫氧化鈉溶液,調節酸鹼度,再按常規檢測.蒸餾法-USP611,MethodⅠB1068,BenzalkoniumChlorideSolution,50Percent,NFAlcoholcontent≦10.0%25℃下量取樣品25.0ml,加水100ml,365℃下蒸餾.餾出物大約23ml時,停止蒸餾,在25℃下定容到25ml.因餾出物渾濁,加入CaCO3振搖,過濾.使用10ml比重瓶測量25℃下乙醇比重,折算出乙醇含量.結果:乙醇餾出物比重@25℃:0.9938乙醇含量:4.2%氣相色譜法(GC)-ACS10th:EthylAlcohol.HP612,ReagentAlcohol,Denatured,ExceedsA.C.S.Specification,HPLCGradeSpecifications,HPLCGradeApplication:EthylAlcoholcontent≧95.0%GCmethodColumn:methylsiliconecapillaryColumntemperature:40℃for5min,programmedto220℃at10℃/min,hold2minInjectortemperature:150-220℃Detectortemperature:250℃Samplesize:0.2μlsplitlessCarriergas:heliumat3ml/minDetector:TCDMeasuretheareaunderallpeaks,andcalculatetheethylalcoholcontentinareapercentHP612,ReagentAlcohol,Denatured,ExceedsA.C.S.Specification,HPLCGradeSpecifications,HPLCGrade氣相色譜法(GC)-USP611,Alcoholdetermination,MethodⅡa.BE115,BelladonnaTinctureApplication:Alcoholcontent:65.0%-70.0%.Column:DB-624Columntemperature:120℃,Injectortemperature:210℃Detectortemperature:210℃Samplesize:5μlCarriergas:nitrogenorheliumDetector:FIDStandardsolution:5mleachUSPAlcoholRSandUSPacetoneRStogetherdilutedbywaterto50ml.Teststocksolution:Dilutesamplestepwisetogeta2%alcoholsolution.Testsolution:5mleachteststocksolutionandUSPacetoneRStogetherdilutedbywaterto50ml.SystemSuitabilityTest:R≧2(alcoholtoacetone)tailingfactor:≦2.0(alcohol)RSD≦2.0%(ratioofalcoholtoacetone)BE115,BelladonnaTincture,AlcoholContent氣相色譜法(GC)-USP467ResidualSolventsApplication:Alcoholcontent:﹤5000ppm.Column:DB-624Columntemperature:40℃for20min,programmedto240℃at10℃/min,hold20minInjectortemperature:140℃Detectortemperature:250℃Samplesize:5ml,headspaceCarrierga