3种苦参生物碱对小鼠的毒性作用研究

,2009,29(4):0818-0823ActaBot.Boreal.2Occident.Sin.:100024025(2009)042081820633,3,(,712100):,3():(1),+,(c.f)59.1,+,c.f37.3,;+,c.f13.6,(2),3(WBC)(PLT)(GR);,(BUN)(Cr)(P0.01),(ALT)(AST)(P0.01);:(P0.01),(P0.01P0.05P0.05),3,:;;;:Q946.88;Q949.99:AToxicityofThreeSophoraflavescensAitAlkaloidstoMiceSONGBing,HANChong2xuan3,ZHANGHong2li(CollegeofForestry,NorthwestA&FUniversity,Yangling,Shaanxi712100,China)Abstract:ThetoxicityofthreeSophoraflavescensAitalkaloids(Oxymatrine,sophoridine,matrine)tomiceweresystematicallyresearchedinthispaper.Resultsareasbelow:thetoxicityofSophoraflavescensAitalkaloidswhichactedonmicewereincreasedaftermixture.Inmatrineandsophoridinegroup,c.fvaluewas59.1.Inoxymatrineandmatrinegroup,c.fvaluewas37.3,showingthesynergyeffects;Inoxymatrineandsophoridinegroup,c.fvaluewas13.6whichindicatedthathadadditiveeffect.TheresultsofbloodroutinedeterminationshowedthatWLT,PLTandGRwerehigherthanthoseofcontrol(P0.01).Examiningthebloodbiochemicalindicators,intheoxymatrineandsophoridinegroup,BUNandCrofsampleswereallhigher(P0.01)thanthoseofcontrol,Inthematrinegroup,thequalityofALTandASTwerehigherthanthoseofcontrol(P0.01).Thecoefficientsofkidney,heart,liverweresignificanthigherthanthoseofcontrolfromthestatisticalresultsoforgancoefficients(P0.01,P0.05,P0.05).Accordingtotheaboveresults,thetargetorgansonwhichSophoraflavescensAitalkaloidsactedwerekidneyandliver.Keywords:alkaloidsfromSophoraflavescensAit;mouse;activityrelationship;toxicity(SophoraflavescensAit),3:2008212203;:2009203223:(2006BAD08A1004);(2006249);(200704012):(1981-),,3:,,E2mail:[e-mail],,,[1],,,,[2],,3,3,1[3],,[4]3,,11.1:(Musmusculus),ICR,18.022.0g,,(223),50%70%,3,98%[4]1.21.2.13[5]:30%70%,(),14,,,,7d,(Pm)=1-(1-Pa)(1-Pb)PaPb(c.f)=-100c.f,c.f20,c.f-20,-20c.f201.2.23(1),(),(502512.3mgkg-1);(1507537.5mgkg-1);(62.531.315.6mgkg-1)1014,,12h,0.5mL20g-1,1,5,11[527](2);,(3),,(RBC)(WBC)(LY)(MO)(GR)(HGB)(PLT),(ALT)(AST)(TP),(BUN)(Cre)[8,9]Uritest23000;CL8000:µSD,,SPSS13.0Excel(4)(2d),(5),,,,;,=22.131,+9184,:3,c.f59.1,,+,c.f37.3;+c.f13.6,,32.232.2.113,,,4(P0.01);68(P0.05);,,,,,2.2.246328,26313Table1Theresultsof3alkaloidsactivityrelationshipswithmouseMedicationDose/(mgkg-1)Theorymortality/%Actualmortality/%c.fOxymatrine260-30-Matrine180-30-Sophoridine105-20-+Oxymatrine+matrine260+180517037.3+Oxymatrine+sophoridine260+105445013.6+Matrine+sophoridine180+105447059.11Fig.1Theeffectofsophoridinetomousegrowth2Fig.2Theeffectofmatrinetomousegrowth3Fig.3Theeffectofoxymatrinetomousegrowth4Fig.4Theaveragefodderofsophoridinegroup028295Fig.5Theaveragefodderofoxymatrinegroup6Fig.6Theaveragefodderofmatrinegroup(P0.05);810,,;,:,2.2.32,,,(P0.01)(P0.05);,;3(P0.05),,,(P0.05),3,;;,,,;,,,2.2.43(3),3WBCPLTGRWBC:(1):,;(2);(3):2Table2TheresultofinternalorganscoefficientMedicationDose/(mgkg-1)HeartLiverLungSpleenKidneySophoridine50.00.690.05337.560.32330.810.160.490.06331.970.323325.00.660.05337.580.46330.750.040.370.011.930.153312.30.670.03337.640.29331.190.230.420.0231.960.0733Matrine62.50.580.0236.890.250.850.100.400.032.040.113331.30.570.0237.520.16330.780.030.410.0231.970.193315.60.710.05336.880.270.760.040.420.0231.980.1533Oxymatrine150.00.690.02337.250.1930.880.1230.370.012.100.193375.00.660.06337.310.2030.810.0230.430.0232.040.163337.50.610.05337.310.2230.820.0330.370.032.030.1433CK0.530.066.970.250.790.130.400.051.710.05:3330.050.01Note:3and33indicatesignificantdifferencebetweentreatmentandcontrol(CK)at0.05and0.01level,respectively.Thesameasbelow.1284,:33Table3BloodroutineinspectionMedicationDose/(mgkg-1)RBC(1012/L)WBC(109/L)HGB/(g/L)PLT(109/L)DC/%LYMOGRSophoridine50.07.700.656.450.43314613271203380.48.69.61.510.501.53325.08.140.7237.650.53315117325316376.57.711.22.13312.701.83312.38.370.66336.950.6331581233292223380.48.09.91.59.701.633Matrine62.57.980.776.100.43314615269153384.49.039.61.77.151.0331.37.770.587.000.53314312282103379.68.09.61.710.901.83315.68.530.49338.000.6331571433310323382.98.09.01.28.051.333Oxymatrine150.07.780.577.750.53314112306253381.58.910.02.038.051.23375.07.890.676.600.43314513265153385.39.238.61.27.601.2337.57.760.458.550.63314610279183382.98.09.91.537.201.13CK7.960.644.700.3146122451883.49.09.61.47.000.94()Table4Bloodserumzymology(Bloodbiochemistrytarget)MedicationDose/(mgkg-1)AST(U/L)ALT(U/L)BUN/(mmol/L)Cr/(mol/L)TP/(g/L)Sophoridine50.0243.836.435.05.611.32.33350.95.23360.15.825.0220.930.427.74.911.02.03348.06.73364.56.5312.3210.523.123.05.110.92.53346.06.23362.86.1Matrine62.5459.250.13381.011.3337.801.93333.55.953.44.631.3362.334.73362.49.3336.401.537.56.453.94.615.6300.436.53358.25.2337.101.837.96.154.85.1Oxymatrine150.0121.319.825.94.210.32.23370.27.63373.46.93375.0281.038.2342.76.19.401.73365.45.23360.56.537.5221.629.630.25.19.001.63357.56.23362.25.8CK243.836.448.87.27.001.141.35.461.45.2:U.Note:U.Enzymeactivityunits.,GR()[10]PLT,;,[11];3RBCHGBDCLYMO,2.2.53(4),,BUN,Cr,(P0.01),;,ALTAST,(P0.01),3(1)3(2),,(),[12,13],[14,15](3),3,22829,,,6.3%,16.98%[16];,,,(4),3,3;,3,;3WBCPLTGR,,;,ALTASTTP;BUNCr,3:[1].()[M].:,1992:333