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48620033*(,100093.*,E-mail:yxjing@ns.ibcas.ac.cn),:.,:,,;,;nodD3;;;,,;,,.,,(),,.80%,,.(FAO)1995,2×106t(4×108t),3/4.,.,,,,.3016,6.4×108t,64×108t.,,.,;,,.,,,.,,,27%,,.3:(1),,;(2),,,,,;(3),,,,.,.,,.,,,.1,,,,.,:;;;48620033536().,19700,2800,15%,0.5%[1].,,(30%),,,.(),,,60%[2].().,(Streptomycesthermoautotrophicus)[3],.,.,.1400.,,,,[4~7],.,,37,(Rhizobiumhanaese)1314..,.,.1.2(Klebsiellapneumoniae),,nifC,7nifnifA[8~10]..,,dctABD[11];,[12,13];(AzospirillumbrasilenceYu62),[14].(nifA)(NifA),nifA,(Bradyrhizobiumjaponica)(Enterbactercloacae),[15~19].[20,21].,(nifL)[22,23].Yu62,[24~26].,21.1%,8.5%.20%.,nodD3[27,28],.,nodD3[29],2NtrC[30];[31];,,,[32,33].,(Sinorhizobiumhuakui)[34,35],[36,37].,,.1.348620033[38,39].Schmid[40]FeMoconifB-Av1,41,(funnel),FeMoco,.,[41].:HD[42];N2,H2,[43].1992XMoS3FeFeS3Fe3[38],3S(FeMo).,[44];,,N2H2,[45~47].,.1.4“”114.1937,65.2070,,,.,W.Newton,200314.,:,;,,,..,,.,,,,.2,.2.1(),().,,.,,,,,[13,14,20,21].2.2,..,,.,,?,,,.2.3,...,.,RNAσ54σ70.CRP(carbonreceptorprotein)σ5448620033538σ54dctAglnAp2[36,37],.,CRP-cAMPnif,CRP[48,49].C3,10%,5%,[50].nifA,,[51,52],,,.,.,,30%[53]..C4,C4.,C4,[54~56],C4.2.4..[57],(Rhizobiumloti).MedicagosativaLin)(GlycinemaxLin)(Lotuscorniculatu)[58~60]..,[61~63].,,.,[64]..,RNA,,,.,,,.,..(:2001CB108904).1DenarieJ,RocheP.Rhizobiumnodulationsignals.In:VermaDPS.MolecularSignalsinPlant-MicrobeCommunications.BocaRaton/AnnArber/London:CRCPress,1991.296~3242PliveriraALM,UrquiagaS,DobereinerJ,etal.Biologicalnitrogenfixation(BNF)inmicropropagatedsugarcaneplantsinoculatedwithdifferentendophyticdiazotrophicbacteria.In:PedrosaFO,HungriaM,YatesMG,eds.NitrogenFixation:FromMoleculestoCropProductivity.Dordrecht/Boston/London:KluwerAcademicPublishers,1999.4253RibbeM,GadkariD,MeyerO.N2fixationbyStreptomycesthermoautotrophicusinvolvesamolybdenum-dinitrogenaseandamanganese-superoxideoxidoreductansethatcoupleN2reductiontotheoxidationofsuperoxideproducedfromO2byamolybdenum-COdehydrogenase.JBiologicalChemistry,1997,272:26627~266334TanZY,XuXT,WangET,etal.PhylogenicandgeneticrelationoshipsofMesorhizobiumtianshaneneseandrelatedrhizobia.IntJSystBacteriol,1997,47:874~8795WangET,vanBerkumP,SuiXH,etal.DiversityofrhizobiaassociatedwithAmorphafruticosaisolatedfromChinesesoilsanddescriptionofMesorhizobiumzmorpphaespnov.IntJSytsBacteriol,1999,49:51~656TanZY,WangET,PengGX,etal.CharacterizationofbacteriaisolatedfromwildlegumesintheNorth-WesternregionsofChina.IntJSystBacteriol,1999,49:1457~14697YanAM,WangET,KanFL,etal.SinorhizobiummelilotiiassociatedwithMedicagosativaandMelilotusspp.IntJSystBacteriol,2000,50:1887~18918ShenSC.Organizationandregulationofnitrogenfixationgenes:1974~1995.In:KungSD,YangSF,eds.DiscoveriesinPlantBiology.Vol.Dordrecht/Boston/London:WorldScientificPress,2000.383~3929,,,.nifA(Klebsiellapneumoniae)glnNif-.,B,1983,(8):688~69610HuB,ZhuJB,ShenSC,etal.ApromoterregionbindingproteinandDNAgyraseregulaeanaerobictranscriptionofnifALinEnterbactercloacae.JBacteriol,2000,182:3920~392311WangYP,BirkenheadK,BoestenB,etal.GeneticanalysisandregulationoftheRhizobiummelilotiigenescontrollingC4-dicarboxylicacidtransport.Gene,1989,85:135~14312BosworthAH,WilliamsMK,AlbrechtKA,etal.AlfalfayieldresponsetoinoculationwithrecombinantstrainsofRhizobiummelilotiiwithanextracopyofdctABDand/ormodifiednifAexpression.ApplEnvironMicrobiol,1994,60:3815~383213,,,..,1995,1:28~3314,,,.EnterobactergergivuaeE7.,2000,33:72~7715ShenSC,wangSP,YuGQ,etal.ExpressionofthenodulationandnitrogenfixationgenesinRhizobiummelilotiiduringdevelopment.Genome,1989,31:354~36016,,,.(Rhizobiummeliloti)nifA.,B,1990,(3):261~26617DengXP,ShenSC.StructureandoxygensensitivityofnifLApromoterofEnterobactercloacae.ScienceinChina,SerB,1995,48620033(1):60~6618,,,.nifA.,2001,46(23):1984~198719,,,.P1(DS).,C,1996,26(2):100~10620..,1995,21:302~30621,,..,1995,1:93~9822HuB,ZhuJB,ShenSC,etal.ApromoterregionbindingproteinandDNAgyraseregulaeanaerobictranscriptionofnifALinEnterbactercloacae.JBacteriol,2000,182:3920~392323XiaoH,ShenSC,ZhuJB.NifL,anantagonisticregulatorofNifAinteractingwithNifA.ScienceinChina,SerC,1998,41(3):303~30824,,.nifA.,1995,11:385~38825,,.Yu62dragTG.,1999,15:281~28726,.Yu62dragTG.,1997,13:343~34927,,,.nodD3P1.,1999,44(21):2308~231228YuGQ,ZhuJB,GuJ,etal.EvidencethatthenodulationregulatorygenenodD3ofRhizobiummelilotiistranscribedfromtwoseparatepromoters.ScienceinChina,SerB,1993,36:225~23629,,,..,B,1994,24(10):1053~105930,,,.(Sinorhizobiummeliloti)nodD3P1.,C,2002,32(6):512~51831WangL,LiC,WangQ,etal.ChemicalsynthesisofNodRm-1:thenodulationfactorinvolvedinRhizobiummelilotii-legumesymbiosis.JChemSocPerkinTrans,1994,1:621~62832ZhangJX,JingY,ShenSH,etal.Transformationoftwonitrogen-fixation-relatedplantgenesintotobaccoandtheirexpressions.ActaBotanicaSinica,2000,42:834~84033ZhangJX,WangYP,ShengSH,etal.TransformationofpealectingeneandParasponiahaemoglobingeneintoriceandtheirexpressions.ActaBotanicaSinica,2001,43:267~27434,,,.nifDNA.,1992,37(17):1603~160635,,,.Ra1
本文标题:中国生物固氮研究现状和展望
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