山楂叶悬钩子锈病冬孢子生物学特性研究

高锋(,132101)[目的]研究山楂叶悬钩子锈病冬孢子的生物学特性[方法]采用玻片萌发法[结果]多孢锈菌属灰色多孢锈菌冬孢子萌发温度为0~36,22.5为最适萌发温度;在清水中即可萌发且萌发率较高,对CN源要求不高;pH值范围为4~12时均可萌发;光暗交替利于孢子萌发;孢子密度越小萌发率越高;36为冬孢子致死温度[结论]为锈病病原菌的其他孢子形态和生物学特性的研究奠定一定基础山楂叶悬钩子;锈病;冬孢子;生物学特性S661.5A0517-6611(2010)26-14412-02StudyonBiologicalCharacteristicsofRubusCrataegifoliusRustTeliosporeGAOFeng(JilinAgriculturalScienceandTechnoligyCollege,Jilin132101)Abstract[Objective]TheamiwastostudythebiologicalcharacteristicsofRubuscrataegifoliusrustteliospore.[Method]Slidegerminationmethodwasused.[Result]TheresultsshowedthatgrowthtemperatureofPhragmidium.griseumDie.tteliosporewas0-36,theoptmiumgrowthtemperaturewas22.5;ItcouldgerminateinwaterandthegerminationratecouldbehigherforlessdemandingontheCandNsource;ItcouldgerminatewirhthepHvalueof4-12;Lightalternatingdarkwasconductivetosporegermination;sporegerminationratewashigherwithlesssporedensity;Teliosporelethaltemperaturewas36.[Conclusion]Thestudylaidafoundationforresearchonothersporemorphologyandbiologicalcharacteristicsofrustpathogens.KeywordsRubrscrataegifolius;Rus;tTeliospore;Biologicalcharacteristics高锋(1980-),女,吉林吉林人,硕士,助教,研究方向:植物保护学Emai:[e-mail]20100621,,,,,(PhragmidiumgriseumDie.t),,[1],,,,;910,,,,,,[2],11.1()1.2,6h12h,;,[3-5]1.2.1031222.532.5406,22.51.2.2CN,C;NLLCN,141.2.34h8h8h4h41.2.4pH1mol/LNaOHHClpH246781012,71.2.52,21.2.62ml,30354010min,,22.5,6h12h,11,4,322.10~32.5,032.5,,35;22.5(1)1Table1EffectsoftemperatureonteliosporegerminationTemperature%GerminationrateDifferencesignificant0.05a0.050.01a0.010.016.67aA3.032.33bB12.053.33cBC22.588.00cC32.51.33dD40.00.00dD2.2CN,;CN,NH4Cl(23)2.34,,马树梅况玲玲,JournalofAnhuiAgr.iSc.i2010,38(26):14412-144132CTable2EffectofdifferentcarbonsourcesonteliosporegerminationCTypeofcarbonsource%GerminationrateDifferencesignificant0.05a0.050.01a0.01Glucose81.67aAFructose88.67aASucrose82.67aAMaltose45.33bBLactose50.33bBWater88.00bB3NTable3EffectofdifferentnitrogensourcesonteliosporegerminationNTypeofnitrogensource%GerminationrateDifferencesignificant0.05a0.050.01a0.01NH4NO374.67aANH4Cl51.67aA(NH4)2SO484.67aAGlycine72.33aALLalanine80.00aALLleucine84.00aAPeptone75.33aAWater88.00bB4Table4EffectofdifferentilluminationonteliosporegerminationDifferentillumination%GerminationrateDifferencesignificant0.05a0.050.01a0.01Continuouslight88.00aANolight76.67aA4h8h4hoursdarkand8hourslight91.33abA4h8h4hoursdarkand8hourslight80.00bA2.4pH5,pH4~12,pH7pH2~7,pH,pH7~12,pH,5pHTable5EffectofdifferentpHvaluesonteliosporegerminationpHpHvalue%GerminationrateDifferencesignificant0.05a0.050.01a0.0120.00aA420.00abA667.33bA788.00cB879.00dC103.00eC120.33eC2.56,,82.33%6Table6EffectofconcentrationonteliosporegerminationConcentration%Germinationrate!∀#AverageThin79828682.33Thick72575461.002.67,377Table7FataltemperatureofteliosporeTemperature%Germinationrate355.00363.00370.0037.50.003,,0~36,22.5;CN,;,pH4~12;;;37,,[1]戴芳澜.中国真菌总汇[M].北京:科学出版社,1979.[2]戚佩坤,白金铠.吉林省栽培植物真菌病害志[M].北京:科学出版社,1966.[3]LIUQ,XIAOC.InfluenceofnutrientandenvironmentalfaetorsonconidialgerminationofPotebniamycespyri[J].PhytoPathology,2005,95(5):572-580.[4]XIAOCL,SITTONJW.EffectsofculturemediaandenvironmentalfactorsonmycelialgrowthandpyenidialproductionofPotehoiamycespyri[J].MycolRes,2004,108(8):926-932.[5]廖安平.红花锈病的研究[D].雅安:四川农业大学,2008.[6]高应奇,张建立,袁智兴,等.小麦条锈病药剂防治试验初报[J].内蒙古农业科技,2008(1):85-86.[7]程志锋,杨文香,刘大群,等.60Co射线与EMS对小麦近等基因系TcLr10的复合诱变[J].华北农学报,2008,23(S2):98-101.[8]付宝建,姚占军,王嘉瑞,等.不同抗条锈基因小麦品种(系)农艺性状分析[J].华北农学报,2008,23(S2):235-239.[9]李勇,牛永春.小麦抗条锈基因Yr6分子标记初步研究[J].华北农学报,2007,22(4):193-196.1441338卷26期高锋山楂叶悬钩子锈病冬孢子生物学特性研究