660nm红光提高干细胞移植治疗缺血缺氧脑损伤的效果

SICENCE新闻发布作者：李献超稿号：N20120828004660nm红光提高干细胞移植治疗缺血缺氧脑损伤的效果研究表明骨髓间充质干细胞在体外向神经元的分化率高达78-92%，但其体内分化率较低，这是制约其治疗效果的最大障碍。实验发现红光或近红外光具有促进角化细胞迁移的作用，但红光对骨髓间充质干细胞治疗缺血缺氧脑损伤有促进作用吗？中国重庆大学吴小鹰博士所在团队发表的一项研究发现，骨髓间充质干细胞经波长660nm、光功率密度为60mW/cm2发光二极管红光进行连续照射后，向缺血缺氧损伤的原代神经元迁移数量显著增加。同时给予缺血缺氧脑损伤模型大鼠腹腔注射骨髓间充质干细胞，并以660nm、60mW/cm2发光二极管红光连续照射头部7d。缺血缺氧损伤大鼠学习能力增强。因此作者认为以660nm发光二极管红光照射可增强骨髓间充质干细胞迁移能力，进而提高缺血缺氧脑损伤的治疗效果。相关文献发表于《中国神经再生研究（英文版）》杂志2014年2月第3期。红光照射40h后，Transwell试验显示，上层培养的骨髓间充质干细胞向下层缺血缺氧损伤的原代神经元迁移Article:660nmredlight-enhancedbonemarrowmesenchymalstemcelltransplantationforhypoxic-ischemicbraindamagetreatment,byXianchaoLi1,WenshengHou1,2,XiaoyingWu1,2,WeiJiang3,HaiyanChen2,NongXiao3,PingZhou2(1KeyLaboratoryofBiorheologicalScienceandTechnology,MinistryofEducation,CollegeofBioengineering,ChongqingUniversity,Chongqing,China;2ChongqingEngineeringResearchCenterforMedicalElectronicsTechnology,Chongqing,China;3RehabilitationCenter,Children’sHospitalofChongqingMedicalUniversity,Chongqing,China)LiXC,HouWS,WuXY,JiangW,ChenHY,XiaoN,ZhouP.660nmredlight-enhancedbonemarrowmesenchymalstemcelltransplantationforhypoxic-ischemicbraindamagetreatment.NeuralRegenRes.2014;9(3):236-242.欲获更多资讯：NeuralRegenRes660nmredlight-enhancedBMSCstransplantationforhypoxic-ischemicbraindamageAseriesofpreviousstudiessuggestedthattheneuronaldifferentiationrateofbonemarrowmesenchymalstemcellsduringtheinvitroculturereached78–92%,buttheirinvivotransplantationefficiency,andsurvivalanddifferentiationrateswereverylow.Thelowlevelsofbonemarrowmesenchymalstemcelltransplantation,survival,colonizationanddifferentiationefficacygreatlyrestricttheirtherapeuticeffect.Redornear-infraredlightfrom600–1,000nmpromotescellularmigrationandpreventsapoptosis.Thus,Dr.XiaoyingWuandco-workersfromChongqingUniversityinChinahypothesizedthatthecombinationofredlightwithbonemarrowmesenchymalstemcelltransplantationwouldbeeffectiveforthetreatmentofhypoxic-ischemicbraindamage.Theyfoundthat,aftera40-hourirradiationunderredlight-emittingdiodesat660nmand60mW/cm2,anincreasingnumberofgreenfuorescence-labeledbonemarrowmesenchymalstemcellsmigratedtowardshypoxic-ischemicdamagedprimaryneurons.Meanwhile,neonatalratswithhypoxic-ischemicbraindamageweregivenanintraperitonealinjectionof1×106bonemarrowmesenchymalstemcells,followedbyirradiationunderredlight-emittingdiodesat660nmand60mW/cm2for7successivedays.Shuttleboxtestresultsshowedthat,afterphototherapyandboneSICENCE新闻发布作者：李献超稿号：N20120828004marrowmesenchymalstemcelltransplantation,theactiveavoidanceresponserateofhypoxic-ischemicbraindamageratswassignificantlyincreased,whichwashigherthanthatafterbonemarrowmesenchymalstemcelltransplantationalone.Experimentalfindings,publishedintheNeuralRegenerationResearch(Vol.9,No.3,2014),indicatethat660nmredlightemittingdiodeirradiationpromotesthemigrationofbonemarrowmesenchymalstemcells,therebyenhancingthecontributionofcelltransplantationinthetreatmentofhypoxic-ischemicbraindamage.Aftera40-hourirradiationunderredlight-emittingdiodesat660nmand60mW/cm2,anincreasingnumberofgreenfuorescence-labeledbonemarrowmesenchymalstemcellsmigratedtowardshypoxic-ischemicdamagedprimaryneurons.Article:660nmredlight-enhancedbonemarrowmesenchymalstemcelltransplantationforhypoxic-ischemicbraindamagetreatment,byXianchaoLi1,WenshengHou1,2,XiaoyingWu1,2,WeiJiang3,HaiyanChen2,NongXiao3,PingZhou2(1KeyLaboratoryofBiorheologicalScienceandTechnology,MinistryofEducation,CollegeofBioengineering,ChongqingUniversity,Chongqing,China;2ChongqingEngineeringResearchCenterforMedicalElectronicsTechnology,Chongqing,China;3RehabilitationCenter,Children’sHospitalofChongqingMedicalUniversity,Chongqing,China)LiXC,HouWS,WuXY,JiangW,ChenHY,XiaoN,ZhouP.660nmredlight-enhancedbonemarrowmesenchymalstemcelltransplantationforhypoxic-ischemicbraindamagetreatment.NeuralRegenRes.2014;9(3):236-242.