桉树枯萎病菌的生物学特性及对杀菌剂的敏感性试验

*122(1.526060;2.)对桉树枯萎病菌的生物学研究结果显示,病菌的最适生长温度为25,最适pH值为5.5,光照对生长影响不大菌丝致死温度为65糖源对分生孢子萌发无明显作用杀菌剂毒力测定显示根腐灵宝宁对菌丝生长抑制较强,克露和宝宁对孢子发芽有较强的抑制作用桉树尖孢镰刀菌生物学特性杀菌剂毒力:S792.39:A:1006-4427(2010)05-0075-04StudiesonBiologicalCharactericsofPathogenofEucalyptusWiltandItsSensitivitytoFungicidesWangYan1WangJun2SunSi2(1.BeilingMountainForestFarm,Zhaoqing,526060;2.CollegeofForestry,SouthChinaAgriculturalUniversity)AbstractThebiologicalcharacteristicsofpathogenofeucalyptuswilt(Fusariumoxysporum)werestudiedinthispaper.Theresultsshowedthattheoptimumtemperatureforpathogengrowthwas25,andtheoptimumpHvaluewas5.5.Lightconditionwasofnoevidenteffectonthegrowthoffungus.Thelethaltemperatureformyceliawas65.Nosignificanteffectwasfoundforsugarsourcesonthegerminationofconidia.TheresultsoftoxicitytestoffungicidesdemonstratedthatGenfulingandBaoninghadstrongerinhibitiveeffectonthepathogengrowth,whileKeluandBaoninghadstrongerinhibitiveeffectonthegerminationofconidia.Keywordseucalyptus,Fusariumoxysporum,biologicalcharacteristics,fungicide,toxicity(Fusariumoxysporum)2007[1],,[2],,11.1,50%(),60%(),40%(),72%(),75%(),95%(),72%(),70%(())752010265*/:(4400H08065)1.21.2.1温度试验4358,4,6mmPDA,,1.2.2pH值试验0.1MHClNaOHPDApH,pH4.0~11.012,4,6mmpHPDA,25,1.2.3光照试验3,24h12h24h,25,1.2.4菌丝致死温度测定:40656,10min60min6106mm,,,,,PDA25,3d:,6mm5mL1.2.5分生孢子致死温度测定1mL50654,10,2030min,25,4,24h1.2.6糖源对孢子萌发的影响1%2%,,,25,41.31.3.1杀菌剂对菌丝生长的抑制测定[34]PDA,4,PDA6mmPDA,25,6d,(%)=(-)/!100%1.3.2杀菌剂对孢子萌发的抑制测定,,,25,24h,(%)22.116d,15~32,25~30,251PDApH6d2.2pH1pH,pH4~11,,pH5.576:2.3,(2),2PDA36d24h2.4,,6510min;,6530min5010min81.3%,20min,7.8%;6530min,1.8%2.53,2.61,,11(g/mL)(%)2(g/mL)(%)3(g/mL)(%)4071.308082.9420084.342564.2110079.3550084.4328816.8972019.42144026.546082.7612085.2830086.7675056.06125056.68250060.6120014.3280018.04160042.0410048.5680079.88160083.6345041.2990054.35180061.252.72,95.4%,,4g/mL,07720102652:%0.5g/mL4g/mL40g/mL35.70089.867.521.783.079.976.383.964.86.491.782.377.75.00095.495.486.888.4003,15~32,pH4~11,20~3065,6530min,pH,,,,,8,,,,[1],,,.[J].,2007,25(5):5961.[2].[J].,2008(12):3839.[3].[M].:,1993:5259.[4],,.[J].,2004,43(3):142143.78: