植物维生素e合成及其生物技术改良3

E333(100037)E,,E,E,E,E,EE:20032092123(200310028112)33:[e-mail],,2,2,22,[1],,,,[2]E,,,,,E,,,[3],,,,,2,E100%,50%,10%3%,E,,[4]E,E,,E,,,E,E,E,1E111E,E,(HPP)(HPPD),(HGA,2,52)[5],,,,(HGA)(HPT),(PDP),2226221,42,;(GGDP),,2226221,42,[6][7],2226221,42,2;2,3225221,42(DMPQ),,2;2,3225221,42(DMGQ),2[2],,241CHINABIOTECHNOLOGY20041©1994-2006ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(2TMT),[8]E:112E,4E(HPPD)(EC1113111127)(HPP)(HGA),HPPDcDNA[5],25%95%;50kDa,445,HPPD17%27%,HPPD58%70%[9]HPPD1,PDS1,PDS1HPPD[10](HPT)[6],HPT,,cDNAcDNA,cDNA(GenBankno.AF324344)389,HPT41%,,HPTN295,HPT,9536VTE1[2],4,RSW1CAT2a,,,BACF4D11(GenBankno.AL022537)At4g32770VTE1,slr1737(DNA5212%,4111%),slr1737slr1736,slr17362,,slr1737,,,slr1737,At4g32770N2,N2742(2TMT),[8](PCC6803)295%,2TMTHPPD,331:E©1994-2006ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(ORF),10,,,2TMTORF,,2(2,4)22C2,ORF22,,ORF2TMT2TMT66%cDNA,2TMT,2TMT[8]2EE,,E,,2,,,2112TMTcDNADC3,,,(pDC3)297%,290%,2TMT280,2TMT,22TMT[8]2TMT,,22,E829,E,,2,2,E,22(HPT),,,,HPT10,414;,HPT4,40%,2[7]HPT2TMT,,E12,,EHPT2TMT,2,2TMT,22,E;,HPT2TMT,E,,,,,212,29116416gPg,2131612817gPg,2111866gPg,243229gPg,280%,220%,2240286gPg,2201061104,QTL,,22,20145915gPg,231311416gPg;201819213gPg,24137286151gPg,RFLPSSR4324©1994-2006ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[11],8QTL,,3E,E,,2,2,227%70%,22011,2TMT,22,,2,EE,,,,,,,E,E,,;,,,,[1]FryerMJ.TheantioxidanteffectsofthylakoidvitaminE(2tocopherol).PlantCellEnviron,1992,15:381392[2]PorfirovaS,BergmullerE,TropfS,etal.IsolationofanArabidopsismutantlackingvitaminEandidentificationofacyclaseessentialforalltocopherolbiosynthesis.PNAS,2002,99(19):1249512500[3].E.,2002,4:2426[4]TraberMG,SiesH.VitaminEinhumans:demandanddelivery.AnnuRevNutr,1996,16:321[5]NorrisSR,ShenX,DellaPennaD.ComplementationoftheArabidopsispds1mutationwiththegeneencodingp2hydroxyphenylpyruvatedioxygenase.PlantPhysiol,1998,117:13171323[6]CollakovaE,DellaPennaD.IsolationandfunctionalanalysisofhomogentisatephytyltransferasefromSynechocystissp.PCC6803andArabidopsis.PlantPhysiol,2001,127:11131124[7]CollakovaE,DellaPennaD.HomogentisatephytyltransferaseactivityislimitingfortocopherolbiosynthesisinArabidopsis.PlantPhysiol,2003,131(2):632642[8]ShintaniD,DellaPennaD.ElevatingthevitaminEcontentofplantsthroughmetabolicengineering.Science,1998,282:20982100[9]GarciaI,RodgersM,LenneC,etal.Subcellularlocalizationandpurificationofap2hydroxyphenylpyruvatedioxygenasefromculturedcarrotcellsandcharacterizationofthecorrespondingcDNA.BiochemJ,1996,325:761769[10]NorrisSR,BarretteTR,DellaPennaD.GeneticdissectionofcarotenoidsynthesisinArabidopsisdefinesplastoquinoneasanessentialcomponentofphytoenedesaturation.PlantCell,1995,7:21392149[11]RochefordTR,WongJC,EgeselCO,etal.EnhancementofvitaminElevelsincorn.JournaloftheAmericanCollegeofNutrition,2002,21(3):191198MolecularBiologyandBiotechnologyImprovementofVitaminEBiosynthesisinPlantHUYing2kao(BiologyDepartmentofCapitalNormalUniversity,Beijing100037,China)AbstractVitaminE,anantioxidant,playsaveryimportantroleinplant,animalandhumanbeing.PlantisthemainresourceofthevitaminEforhumanbeing,soitisimportantforustoclonetherelativeenzymegenesandimprovevitaminEcontentinplant.ThebiosynthesisofvitaminE,cloningofrelativeenzymegenesandbiotechnologyimprovementofthevitaminEcontentarereviewed.KeywordsPlantVitaminEGenecloningGeneengineeringMolecularmarker531:E©1994-2006ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.