模拟淀粉废水厌氧发酵生物制氢

382()Vol.38No.220063JOURNALOFSICHUANUNIVERSITY(ENGINEERINGSCIENCEEDITION)Mar.2006:100923087(2006)02200552051,1,2(1.,610065;2.,150090):,(OLR)pH,;,pH3.9,22.0kgCOD/(m3d);87.5mlH2/g,pH3.54.3,pH;pH4.35.0,pH4.3,80mlH2/g,:;;:X24:AAnaerobicFermentativeHydrogenBio2productionfromSimulatedStarchWastewaterLIUMin1,CHENYing1,RENNan2qi2(1.SchoolofArchitectureandEnvironment,SichuanUniv.,Chengdu610065,China;2.SchoolofMunicipalandEnvironmentalEng.,HarbinInst.ofTechnol.,Harbin150090,China)Abstract:Theexperimentsofanaerobicfermentationhydrogenbio2production(HBP)fromsimulatedstarchwastewaterwereconductedincontinuousandbatchreactor.Effectsoforganicloadingrate(OLR)andpHonhydrogenproductioncapacityandliquidproductswereinvestigated.ThebiogasyieldandhydrogenyieldincreasedwiththeincreasingofOLRinCSTR.Aftertheethanol2typefermentationwasobtained,atpH3.9,theCSTRobtainedmaximumbiogasyieldandhydrogenyield(87.5mlH2/gstarch)simultaneouslywhentheorganicloadingratewasat22.0kgCOD/(m3d).Inbatchreactor,hydrogenyieldsincreasedwiththeincreasingofinitialpHvaluefrom3.5to4.3,thendecreasedandkeptstabilizationatpH4.3and5.Themaximumhydrogenyieldwas80.0mlH2/gstarchatinitialpH4.3.Theresultsindi2catedthatitspossibletoproducehydrogenfromstarchwastewater.Keywords:hydrogenproduction;OLR;simulatedstarchwastewater,2002560104t2002560104t:2005-07-20:(200442):(1972-),,.:.COD50006000mg/L,8.28.5t/t[1],,,,[2],,[3-5],,pH,,,pH[6-8],[8][9],,pH,,,,,(OLR)pH11.1--(CSTR,98240801.3),,,120r/min,,19.2L,(371)1Fig.1Schematicdiagramoftheexperomentalapparatus1.2CSTR,(HRT)6h,pH4.0,180g/d100ml2,40ml,0.8g,35pH3.55.0,pHNaOHHCl2Fig.2Experimentalapparatusofbatchreactor1.3,NPCODNP80051,1.4(SC-7),:2mª5mm,2%H3PO4PorapakQ(50/80),()40ml/min0.5ml3,10%(HT-SP502),:2mª5mm,2%H3PO4GDX-103(6080),()30ml/min,()30ml/min,()360ml/min,190,220,22011000r/min10min,COD,pH,MLSSMLVSS22.1CSTR,12.5gVSS/L3.010kgCOD/(m3d)40d3,22.0kgCOD/(m3d)65()3887.5mlH2/g25.0kgCOD/(m3d),CSTR,,pH,,,NH4+Ca2+,25kgCOD/(m3d),2000mg/L,pH,25kgCOD/(m3d)3Fig.3Theeffectoforganicloadingrateongasandhydrogenyield2.2pH4CSTR()pH,,,,pH4.5,pH4.2,70%,pH4.1,,2359mg/L,1164mg/L40,40,pH,,,30pH3.9,2712mg/L135,pH3.7,,4,,,pH3.74pHFig.4ProcessofH2yieldliquidproductsalongwiththepHvalvebymixedbateria2.3pH5,pH3.55.05pHFig.5EffectofinitialpHonhydrogenproductionrateinbatchculture5,pH3.54.3,pH4.3,80.0mlH2/gpH4.35.0,;pH4.55.0,,pH4.3,CSTR,pHpHCSTR752,:pH,0.4pHpH4.0,,,pH4.0,pH2.4pH1pH3.55.01pHTab.1EffectofinitalpHvalveonliquidproductinbatchculturepH/(mgL-1)3.5090190824733012425103.751103116740645015831844.001209130340567425238424.301387146440164422441194.501521152943462824643585.00183715513836312364638:,(351),,70%,,pHpH5.0,4638mg/L,1837mg/L5,pH4.3,,,pH2.5,pH3.9,100d,pH()pH,6pH,pH3.85pH4.4,pH4.4pH,(a)pH3.85,(b)pH4.4,6pHFig.6MicrobeobservedbySEMindifferentpHvalue3,,pH3.7CSTR,pH3.9,22.0kgCOD/(m3d),87.5mlH2/g,pH3.54.3,,pH4.3,80mlH2/g,pH4.3,pH5,(4638mg/L),(1837mg/L);CSTR,pH4.3,(2712mg/L),(1154mg/L),,pHpH,CSTR,85()38:[1]HuangFuhao,Luodechun.Anaerobic2aerobictreatmentofcornstarchwastewater[J].JournalofXianHighwayUniversity,1999,19(3):139-142.[,.-[J].,1999,19(3):139-142].[2]JinB,VanLeeuwenHJ,PatelB,etal.Utilisationofstarchprocessingwastewaterforproductionofmicrobialbiomassproteinandfungal-amylasebyaspergillusoryzae[J].BioresourceTechnology,1998,66:201-206.[3]LiuMin.Characteristicsoftwo2phaseanaerobicprocesswithhy2drogenbioproductionandmethanogenicreactors[D].Harbin:HarbinInstituteofTechnology,2004.[.-[D].:,2004.][4]NielsenAT,AmandussonH,BjorklundR,etal.Hydrogenpro2ductionfromorganicwaste[J].HydrogenEnergy,2001,26:547-550.[5]UenoY,OtsukaS,MorimotoM.Hydrogenproductionfromin2dustrialwastewaterbyanaerobicmicroflorainchemostatculture[J].JFermenandBiogen,1996,83:194-197.[6]BrosseauJD,MagaritisA,ZajicJE.Theeffectoftemperatureonthegrowthandhydrogenproductionbycitrobacterinter2medius[J].Biotechnollett,1982(3):307-312.[7]MizunoO,DinsdaleR,HawkesFR,etal.Enhancementofhydrogenproductionfromglucosebynitrogengassparging[J].BioresourceTechnology,2000,73:59-65.[8]LeeYJ,MiyaharaT,NoikeT.EffectofpHonbicrobialhy2drogenfermentation[J].JChemTechnolandBiotech,2002,77:694-698.[9]RenN,WangB,HuangJ.Ethanol2typefermentationformcar2bohydrateinhighrateacidogenicreactor[J].BiotechandBio2eng,1997,54:428-433.()(46):[1]WickertJA,MoteJrCD.Classicalvibrationanalysisofanaxi2allymovingcontinua[J].JAppliedMchanics,1990,57(4):738-744.[2]OzHR,PakdemirliM,BoyaciH.Non2linearvibrationsandstabilityofanaxiallymovingbeamwithtime2dependentvelocity[J].IntJNon2Linearmechanics,2001,36:107-117.[3]HuangJS,FungRF.Dynamicstabilityofamovingstringun2dergoingthree2dimensionvibration[J].InternationalJournalofMechanicsScience,1995,37(1):145-159.[4]PerkinsNC,MoteCD.Three2dimensionalvibrationoftravel2lingelasticcables[J].JournalofSoundandVibration,1987,114(2):325-340.[5]ZhangL,ZuJW.Nonlinearvibrationofparametricallyexcitedmovingbelts[J].JournalofAppliedMechanics,1999,66(2):396-409.[6]FungRF,HuangJS,ChenYC,etal.Nonlineardynamicanal2ysisoftheviscoelasticstringwithaharmonicallyvaryingtrans2portspeed[J].Computer&Structure,1996,61(1):1