水稻谷蛋白突变体的分类及其分子生物学研究进展

(JiangsuJ.ofAgr.Sci.),2010,26(4):874~881,,,.[J].,2010,26(4):874881.田孟祥1,2,陈涛2,张亚东2,朱镇2,赵凌2,赵庆勇2,周丽慧2,王艳平2,王才林2(1.,210095;2.,,,210014):20100114:(2008ZX08001-006);()(200803056);(BE2008354);[CX(09)634];(BK2008347):(1983),,,,(Email)[e-mail]:,(Email)[e-mail]:,,,,,,:;;:S511.01:A:10004440(2010)04087408ResearchProgressinClassificationandMolecularBiologyofGlutelinMutantsinRice(OryzasativaL.)TIANMengxiang1,2,CHENTao2,ZHANGYadong2,ZHUZhen2,ZHAOLing2,ZHAOQingyong2,ZHOULihui2,WANGYanping2,WANGCailin2(1.CollegeofAgriculture,NanjingAgriculturalUniversity,Nanjing210095,China;2.InstituteofFoodCrops,JiangsuAcademyofAgriculturalSciences,JiangsuHighQualityRiceR&DCenter,NanjingBranchofChinaNationalCenterforRiceImprovement,Nanjing210014,China)Abstract:Riceglutelinmutantsarenotonlyimportantgeneticmaterialsforstudyingbiosynthesisandgeneexpressionregulationofstorageproteinineukaryote,butalsoareexcellentgermplasmresourcesinricefunctionalbreeding.Inrecentyears,agreatprogresshasbeenmadeingeneticvariationmechanismandbreedingofriceglutelinmutants.Inthispaper,researchprogressinclassificationandmolecularbiologyofglutelinmutantsinricewasreviewedaccordingtothepublishedreferences.Therelationshipbetweenglutelinmutantsandfunctionalricebreedingwasdiscussed,andstrategiesforfurtherfunctionalricebreedingusingglutelinmutantswerealsoproposed.Keywords:rice(OryzasativaL.);glutelinmutant;functionalricebreeding,60%~80%,,,,,874,,,,1,,,57000,(Proteinbody,PB),2,,,37000~3900022000~23000[13],,SDSPAGE,[45]57000(57H)31.1Iida[6](Nihonmasari),NM67,SDSPAGE,,22000~2300037000~39000,1300026000;,NM67,,1,4,1,33[78],,,,[910],Iida[11](Koshihikari)8(Norin8)88KG30756M539;,Satoh[12][1314]12(1),[15]16168SDSPAGE,W3660[16],IRAT144,[17]3000,(1)1.257H57H,,570001986,Shiraishi[18]257HHO24TAL2,1988,Kumamaru[19](Kinmaze)657HCM615CM935CM1787EM25EM61EM30557000,EM61EM305,,CM1787,1%,CM1787,,,57000CM1787,PB,PBI,05m,57000[20],57H875:,[21]N22Q40411Table1ThereportedlowglutelinmutantsinriceNM671,3[7][8]88KG307561![11]M53988KG3091388KG3095888KG309932![11]87KE0.13972[11]87KG2013089WPKG30163![11]90WPKE0.15613[11]EM2781,2[12]EM4131[12]EM6593,2[12]CM1707TCM221TCM11701,2[12]EM895EM491[12]CM1530EM335EM336EM13171[13]EM278TCM26221,2[14]CM1707TCM1170TCM12242,2[14]CM439EM659TCM221TCM2223,2[14]W3660[15]IRAT144[16][17],Satoh[22]14001957HSDSPAGE,[15]16160W204W379257H,1000257H,,57000,,1.3,,,,,74~75,,(75),(76)[23],,1988Kumamaru[19](Kinmaze),2CM1834CM21,,2,,,57H,87620102642.1Iida[11],type1type2type3Glu1Glu2Glu3RFLP,2101Southern,Glu1B,Glu2A,13%,75%;260001600013000,LGC1,,Miyahara[8]LGC1,LGC1,Glu1,2XNpb243G365,XNpb24385cM,LGC1Kusaba[2425]LGC1RNA,,2998%GluBGluB4GluB5DNA;LGC1,GluB5335kbDNA,GluB5,GluB4RNA,Lgc1,LGC1Fujio[26]RTPCR,Lgc1Wang[27]W3660,(SSR),2SSR2001RM1358,11cM38cMW3660Lgc1,,LGC1GluB4GluB5RNA;W3660,GluB5,,W3660LGC122.257H,57000,,,57H,1057H(2),Esp2Glup1Glup2Glup5Glup7,Glup4Esp2Glup1Glup2Glup3[22,29,3235],257HTable2Locatedandcloned57HmutantgenesinriceEsp2CM1787EM44EM74711[28]Glup1EM619[29]Glup2EM3059[29]Glup3EM8564[30]Glup4EM956EM96012[31]Glup5EM675EM677PMT1912[32]Glup6EM9393[33][34]Glup7CM9354[35]OsRab5aQ404112[21]OsVpe1W3794[36]877:CM178757H,11Esp2,(Proteindisulfideisomerase,PDI)Western,Esp2PDIPDI,57000,,[20,28]Q4041,,,,,N22,,,Q4041Q40412F212(Bacterialartificialchromosome,BAC)OJ1584-D0227kb,,,GRad,!OsRab5a∀cDNA,OsRab5a76,cDNA612bp,203,22000,Rab,,Q4041,Q4041,OsRab5a313bp,[21]Q4041,W37957000,W3794OsVpe1,1494bp,494OsVpe11,,W379,Western,,,OsVpe1;,W379[36]OsVpe157H,PDI;osRab5a;OsVpe1,,,57H,3,,,,Iida[37]NM67LGC1,1994,,LGC1,,Lgc1,,Fukuoka[38]231;[39]W36602005,NishimuraLGC1LGCKatsuLGCJun[40],MizumHyogokitanishikiLGC1,Tashukei1001[41],,8782010264,,,,,,[4244],,57H,,Eggum[45],,57HCMl787,57H44.1,,,,,,,,;,,,,,,,4.2,,,,,,,,,,,;,,4.3,(Molecularmarkerassistedselection,MAS),LGC1,,,SSR,W3660[39],,,Lgc12GluB4GluB5Lgc1[46],,,,,:[1]YAMAGATAH,SUGIMOTOT,TANAKAK,eta.lBiosynthesisofstorageproteinsindevelopingriceseeds[J].PlantPhysio,l1982,70(4):10941100.[2]MANDALS,MANDALRK.Seedstorageproteinsandapproachesforimprovementoftheirnutritionalqualitybygeneticengineering[J].CurrentScience,2000,10(5):576579.[3]TANAKATK,DULDULAOJBA,KIMURAY,eta.lThetwosubfamiliesofriceglutelindifferinbothprimaryandhigherorderstructures[J].BiochimicaetBiophysicaActa,2004,1699(12):95102.[4],,,.[J].,2003,36(4):353359.[5]ZHENGTQ,SHENWB,ZHUSS,eta.lStatusandperspectivesontheresearchesofriceglutelinmutants[J].AgriculturalSciencesinChina,2003,2(3):237244.879:[6]IIDAS,AMANOE,NISHIOT.Arice(OryzasativaL.)mutanthavingalowcontentofglutelinandahighcontentofprolamine[J].TheorApplGenet,1993,87:374378.[7]MIYAHARAK,KUSABA,SASSAE,eta.lAnalysisofglutelingeneinricelowglutelinline#LGC1[J].BreedingSc,i1996,46(1):42.[8]MIYAHARAK.AnalysisofLGC1lowglutelinmutantofrice[J].GammaFieldSymposia,1999,38:4352.[9]FURUKAWAS,MIZUMAT,KIYOKAWAY,eta.lDistributionofstorageproteinsinlowglutelinriceseeddeterminedusingafluorescentantibody[J].JBiosciBioeng,2003,96(5):467473.[10]FURUKAWAS,ITOUM,MASUMURAT.Ultrastructureoflowglutelinriceendosperm[J].PlantBiotechnology,2007,24:22722