海洋微生物对多环芳烃的降解Ξ

201Vol.20,No.120012JOURNALOFOCEANOGRAPHYINTAIWANSTRAITFeb.,2001X1,1,1,2,2,1(1.,361005;2.,361005):,ST4;(Phe)(Pyr)(Fla);,50mg/dm3,;200mg/dm3,,.,..:;(PAHs);:Q93919:A:100028160(2001)0120043205(PAHs),,1Fig.1Distributionofsamplingstations,,.,,,,,[1].,;10%18%.,[2],,.8016,.,,.,[1].(1)X:1999212217:(30070157);(99180):(1976),,....11.11.1.12(Phe)(Pyr)(Fla)Sigma.(MSM):(NH4)2SO41000mg/dm3,Na2HPO4800mg/dm3,KH2PO4200mg/dm3,MgSO47H20200mg/dm3,CaCl22H20100mg/dm3,FeCl36H2O5mg/dm3,(NH4)6Mo7O244H201mg/dm3,pH7.2.1.1.2GC,,.1.21.2.16(1),,,4,.1.2.25g50cm3(Na2P2O7,2.8g/dm3),25150r/min,.,5cm345cm3,50mg/dm3.1,,5cm345cm3,1,;5(59).1.2.3.1cm350cm3,..25150r/min;.102550100200mg/dm3..25150r/min,..(250mg/dm3).1.2.4=600nm.1.2.5(GC2FID),(50g/dm3).(SPE).22.1.1,:ST1ST5ST6,;ST3ST4ST2:,ST3ST4ST2;Muller,[3].ST1ST5ST6.,ST3ST4ST2,4420[4];ST1ST5ST6,.Heitkamp(1988),,[5].2.2ST4,,(1215d).,.3956.71234.6677.5g/dm3,92.08%97.52%98.64%;PAHs90%,(2)..Stringfellow,PAHs[6].PAHs,[7],.2Fig.2GrowthchangeondifferentPAHs3Fig.3Growthchangeondifferentconcentrationsofpyrene2.3ST4(10200mg/dm3),25150r/min.,1050mg/dm3,;100mg/dm3,,62.27%;200mg/dm3,(3,1,).50mg/dm3(4),200mg/dm3,,,,,[1].2.4ST4,25150r/min.,;,,,2.337.9d,541:,,(5),.4Fig.4Effectofpyreneconcentrationonmicro2organismgrowthandpyrenedegradation5Fig.5Effectofadditionalnutrientonmicro2organismgrowthonpyrene3Heitkamp,(Cometabolism)[8].,,,.:[1]CernigliaCE.Biodegradationofpolycyclicaromatichydrocarbon:areview[J],Biodegradation,1992,(3):351368.[2]WhiteKL.Anoverviewofimmunotoxicologyandcarcinogenicpolycyclicaromatichydrocarbons[J].EnvironCarciogRev,C4:163202.[3]MullerJG,ChapmanPJ,PritchardPH.Actionofafluoranthene2utilisingbacterialcommunityonpolycyclicaromatichydrocarboncomponentsofcreosote[J].AppliedandEnvironmentalMicrobiolo2gy,1989,55:30853090.[4],,,.[J].,1995,18(4):120124.[5]HeitkampMA,CernigliaCE.Mineralizationofpolycyclicaromatichydrocarbonbyabacteriumiso2latedfromsedimentbelowanoilfield[J].AppliedandEnvironmentalMicrobiology,1988,54:16121614.[6]StromgfellowWT,AitkenMD.Competitivemetabolismofnaphthalene,methylnaphtahalenesandfluorenebyphenanthrene2degradatingPseudomonas[J].AppliedandEnvironmentalMicrobiology,1995,61:357362.[7]WilsonSCAndJonesKC.Bioremediationofsoilscontaminatedwithpolycyclicaromatichydrocar2bons(PAHs):areview[J].EnvironmentalPollutant,1993,81:229249.6420[8]HeitkampMA,CernigliaCE.PolycyclicaromatichydrocarbondegradationbyaMycobacteriumsp.Inmicrocosmscontainingsedimentandwaterfromapristineecosystem[J].AppliedandEnvironmen2talMicrobiology,1989,55:19691973.Degradationonpolycyclicaromatichydrocarbon(PAHs)bymixedmicroorganismisolatedfromcoastalsedimentsGUOChu2ling1,MASKAOUIK1,ZHENGTian2ling1,2,TIANYun2,HONGHua2sheng1(1.KeyLaboratoryforMarineEnvironmentalScienceofMinistryofEducation/EnvironmentalScienceResearchCentre,XiamenUniversity,Xiamen361005,China;2.SchoolofLifeSciences,XiamenUniversity,Xiamen361005,China)Abstract:Thegoalofthispaperistocharacterizemicroorganisms(bacteria)isolatedfromsedi2mentsamplescontaminatedwithpolycyclicaromatichydrocarbon(PAHs).ST4pyreneen2richedmicroorganismwerestudied.Theycanusepyrene,phenanthrene,fluorantheneastheonlysourceofcarbonandenergy.Cellsofthemicroorganismwereinoculatedinmineralsaltmedium(MSM)at10,25,50,100,200mg/dm3pyreneinacetonesolution.Thehighestopticaldensity(OD600)andthehighestdegradationratewereobtainedat50mg/dm3.Additionalnutrientshaveevidenteffectonthegrowthofmixedculture.Keywords:marineenvironment;polycyclicaromatichydrocarbons(PAHs);bacteria741: