猪_犬脾转移因子的理化性质及生物学特性分析

20109239ChinJBiol[e-mail]。、、B。TFTF2~24h1。1-2、、、、3。TFTFTF。TF。。TF、、、、、TFTF4-5。TFTF-PTFCS-TF。1.1.1TF、TF。S852.4A1004-5503201009-0990-03【】、【】、Transferfactor,TF。、TF、pH、、、TF、DNA、、、、、。、TFDNA、、、、A260／280NAI20%、TF2。、TFTF。【】Physico-chemicalPropertiesandBiologicalCharacteristicsofSwineandCa－nineSpleenTransferFactorsKONGQing-boPoliceDogTechniqueSchoolofTheMinistryofPublicSecurity,Shenyang110034,China【Abstract】ObjectiveToanalyzethephysico-chemicalpropertiesandbiologicalcharacteristicsofswineandcaninespleentransferfactorsTFs.MethodsTheTFextractsfromswineandcaninespleenswereobservedforappearance,determinedforpHvalueaswellasproteinandaminoacidcompositions,subjectedtoultravioletspectroscopy,analyzedforTF,DNAandnucleotidecon－tents,thensubjectedtosterility,pyrogen,safety,sensitivityandimmunogenicitytestsanddeterminedforbiologicalactivity.ResultsTheDNA,nucleotideandaminoacidcontentsaswellasaminoacidcompositionsofTFextractedfromswinespleenweredifferentfromthosefromcaninespleen.However,bothswineandcaninespleenTFswerequalifiedinsterility,pyrogen,toxicityandsensitivitytests,showednoimmunogenicityandreachedhighA260／280values.Boththenon-adherenceindexesoftheTFsweremorethan20%.EachmilliliterofswineandcaninespleenTFscontainedabout2activityunits.ConclusionThephysico-chemicalpropertiesandbiologicalcharacteristicsofextractedswineandcaninespleenTFswereconsistentwiththoseofotherTFsreportedinChinaandothercountries,indicatingthatboththequalitiesoftheTFsreachedthelevelsofdomesticandimportedproductsofthesamekind,whichwassuitableforlaboratoryandclinicaluses.【Keywords】Swine;Canine;Spleen;Transferfactor;Physico-chemicalproperties;Biologicalcharacteristics990··20109239ChinJBiologicalsSeptember2010Vol.23No.91.2131.5～2.5kgDHP10300～400g1020g。1.325%、BCADM-276000～10000835-507520。1.4、TF1.4.1。1.4.2pHpH5.4～7.0。1.4.3625%。1.4.4A260A280A260／28027。1.4.5TFBCA8。1.4.6DNA。1.4.7TF。1.4.8。1.5、TF1.5.1《》19957、TF、、37℃7d。1.5.2《》199571.7～3.0kg9315min3ml38℃、TF1h13。1.5.3《》19957102。1、TF5ml／2。7d、。1.5.4《》19957250～350g10、TF0.5ml325114d21d0.5ml15min。1.5.5、TF1ml／14。19。1.6、TFNAI≥20%7。2.2.1、TF2.1.1、TF、、。2.1.2pHTFpH6.5TFpH6.7。2.1.325%、TF。2.1.4TFA260／280=2.423.028／1.250TFA260／280=2.602.857／1.100。A、TF2。2.1.5TFTF1.21mg／mlTF1.54mg／ml。2.1.6DNADNA82.8μg／ml504.8μg／ml。2.1.7332.1μg／ml302.3μg／ml。2.1.8TF17TF151。2.2、TF、TF、、。2.3、TFTFNAI30%TFNAI35%20%。991··20109239ChinJBiologicalsSeptember2010Vol.23No.91、TF%Tab1.PercentageofaminoacidsinextractsofswineandcaninespleenTFsTFTFAsp9.6113.12Thr1.236.88Ser5.129.41Glu16.0226.03Pro4.436.13Gly9.0812.31Ala5.949.27Cys1.15／Val5.356.68Met1.92／Ile3.744.51Leu7.149.43Tyr3.244.42Phe4.626.41Lys8.029.14His3.244.45Arg7.938.313.TF。TFTF、。、10-12、TFDNA、、、、TF12-13、、A260／280TF9。TF2。、TFE14。。TF4×1081TF12。。、。1g1TF。TFTTTTT、TT。TFTF、、、15。16TFTFTF。、TF。1.M.1990:12-17.2．J．、、198255196-198.3．J．200725231-35.4．J．1996384-6.5.J.2006,19160-63.6．M．1993102-104.7．1995M．1996.8．M．200051-54.9．J．199618279-81.10．J．1995221022-23.11．J．19802620-22.12．J．2000222122-123.13．J．19952596-8.14．J．200331487-89.15．J．199424721-23.16．M．198585-88.2009-12-02992··