生物化学与生物物理进展

⋅100⋅Prog.Biochem.Biophys.1999;26(3)()(100101)Q6-331⋅100⋅Prog.Biochem.Biophys.1999;26(3)PMA[3]PMANOPMANO15-20mlHSCH2COONa(l%)Wista25024100g412HBSS(NaCl(8mg/ml).KCl(0.4mg/ml),NaHCO3(0.15mg/ml),NaH2PO4.12H2O(0.152mg/ml),KH2PO4(0.06mg/ml)Glucose(lmg/ml))PMABPCL2mlHBSS106-107/mlPMA10ng/ml-100ng/mlL-arginineNw-nitro-L-arginine5μM-40μML-arginineNw-nitro-L-arginine4PMA137PMANONONADPHPMAPMA22bcNONONONADPHNONO1.(ZhangZL,ZhengYZ,SuZetal).(J.Biomed.Eng.)199411(1)24~302LiHai-tao,ZhaoBao-lu,HouJing-Wu,etal.Two-peakKineticCurveofthechemiluminescenceinPhorbol-inducedMacrophage.BiochemicalandBiophysicaIResearchCommunication,1996,223(2):311~314Ultra-weakChemiluminescenceAnalysisTechno-logyPrincipleandAplication.ZhangZhonglun(InstituteofBiophysicsAcdemiaSinica,Beijing100101,China)Abstract:CLAnalysistechnologywarerapidlydevelopedinrecentyears.Itiswidelyusedinthefieldsoffreeradicleanalysis,chemiluminescence,ultra-weakCLinlivesystem,CLImmunoassay,bioluminescence.PrincipleofUltra-weakChemiluminescenceAnalysisisintroducedinthispaper.Researchandpracticethatwasprocessedbychemiluminescenceanalysiswareexplainedwithseveralinvestigationresults.Keywords:luminescenceAnalysisUltra-weakChemiluminescence2/