生物技术在花卉种质创新中的研究进展

251Vol25No120101JournalofYunnanAgriculturalUniversityJan2010:2008-10-10:2009-06-13*:(2006NG34,2007C118M):(1972-),,,,E-mai:lwql2118@tom1com**Correspondingauthor:(1979-),,,,E-mai:llihan528@126com*李卫琼1,李世峰2,李涵2**(1,650031;2,650205):,,,:;;:S6036:A:1004-390X(2010)01-0118-06TheAdvancesinResearchonFlowerGermplasmInnovationwithBiotechnologyLIWeiqion1,LIShifeng2,LIHan2(1YunnanAgriculturalVocationalandTechnicalCollege,Kunming650031,China;2FlowerResearchInstitute,YunnanAcademyofAgriculturalSciences,Kunming650205,China)Abstract:Withtherapiddevelopmentofbiotechnologyintheglobalagriculturalresearch,biotechnologyfortheapplicationofgermplasminnovationiswildlyusedontheflowersTheadvancesandresearchonCellEngineeringandGeneticEngineeringofflowergermplasminnovationwerereviewedinthispaperInordertoprovideareferenceforthestudy,theproblemsexisted,andthedirectionsofdevelopmentinthefuturewerealsodiscussed.Keywords:flower;biotechnology;germplasminnovation20,,,,,,2110,[1]2007,43885,1965[2],,,,,,111,,,,,,,,1111956,2080,,,,,,,,50,,20[3],[4]60Co,14;[5]60Co,11;[6]60Co,4;[7],,,[8]60Co,;[9]60Co,;[10],;[11]60Co,;[12]60Co,,,,,;[13]60Co,,;[14]60Co,2;[15]60Co,;[16]60Co,;60Co,112201937,BLAKELESSAVERY[17],45%,,2050,2050,40,,,,,,,[18],18,;[19],;[20],1191,:1,1;[21],;[22],;[23],;[24]EMS,NaN3,PLB;[25],;[26],,,;[27],,;[28],;[29],12,,,,,,,,,[30],,,54,[31],[32],,,[33],,1,,[34],,,[35],,13,,,,,,2060,,,2070,,[36],,,59%[37]6,,BRUKHINVB[38],,,,,,ROBERTSM[39]MS,2,,12025,Moon,198710,,,,ZUKER[40]F3!H,;BRUGLIERA[41]F3!5!Hb5,6;YUKIHISA[42]VLOLAF3!5!HDFR;MIZUTANI[43]F3!HDFR,;SUZUKI[44]DFRCHS,;UEYAMA[45]F3!5!HF3!5!H,,SAVINKW[46]ACO,2,;BOVAY[47]ETR-1,6~16d;AIDAR[48]ACO,1~5d;COBBD[49]ETR-1,14d;SHAWJ-F[50]ERS,,,WINEFIELD[51]rolC,;VANDERSALM[52]rol-A,rol-B,rol-C,,LAVY[53]LIS,,,BRUGLIERA[54]ChiA,Fusariumoxysporumfspdianthi;MARCHANT[55]Chitinase∀,,,T-DNA,,,,,,331,2006,#(2006~2020)∃,,,,,,,32,,,,,,,,[][1][J].1211,:,2002,4(6):35-39.[2][J].,2008,19(10):15-17.[3][M].:,2000:234-243.[4],,,14[J].,1996,14(1):57-60.[5],,,[J].,1996,23(3):285-288.[6],,,[J].,2002,(1):35-37.[7],,,[J].,2000,(5):10-11.[8],[J].,1997,(3):50-51.[9],,,[J].,2007,20(5):1138-1139[10],[J].,1997,23(4):336-340.[11],,,[J].:,2007,22(3):332-336.[12],,,Co-60[J].,2008,(2):1-2.[13],,,[J].,2006,(6):226-228.[14],,,[J].,2004,18(3):201-203.[15],,,[J].,2006,(1):1-2.[16],,,(M1)[J].,2002,16(3):148-151.[17][M].:,2005:175-185.[18],,,Siberia[J].,2008,6(2):291-296.[19],,,[J].,2005,21(11):262-264.[20],[J].,2002,18(1):46-50.[21],,,[J].,2008,35(3):443-446.[22],,,[J].,2006,(6):135-136.[23],,,[J].,2007,34(3):729-734.[24],,,[J].,2006,20(2):99-102.[25],,,[J].,2006,28(6):593-598.[26],,,[J].,2007,(2):164-166.[27],,,[J].,2005,32(5):853.[28],,,[J].,2006,(6):259-260.[29],,,[J].,2003,20(3):172-174.[30],[J].,2007,(3):45-46.[31],,,,[J].,2007,(3):67-69.[32],[J].,2001,30(3):62.[33],[J].,2004,(11):22.[34],[J].,1998,18(2):153-156.[35],[J].,1998,20(2):20-21,25.[36],[J].,2003,32(2):55-57.[37],,,[J].,2008,28(1):79-81.[38]BRUKHINVB,BATYGINATBEmbryocultureandsomaticembryogenesisincultureofPaeoniaanmala[J].12225Phytomorphology,1994,44(3-4):151-157.[39]ROBERTSM,SUNDERLANDNPollencultureofPaeonia[J].JohnInnesInstitution68thAnnualReport,1977,68(2):60-61.[40]ZUKERA,TZFIRAJ,BEN-MEIRH,eta.l.Modificationofflowercolorandfragrancebyantisensesuppressionoftheflvonone3-hydroxylasegene[J].MolecularBreeding,2002,9(1):33-41.[41]BRUGLIERAF,TULLD,HOLTON,TA,KARANM,eta.l.Introductionofacytochromeb5enhancestheactivityofflavonoid3!5!-hydroxylase(acytochromeP450)intransgeniccarnation[C]//SixthInternationalCongressofPlantMolecularBiologyQuebec:UniversityofLava,l2000:6-8.[42]KATSUMOTOY,FUKUCHIMIZUTANIM,FUKUIY,eta.l.EngineeringoftheRoseFlavonoidBiosyntheticPathwaySuccessfullyGeneratedBlueHuedFlowersAccumulatingDelphinidin[J].PlantandCellPhysiology,2007,48(11):1589-1600.[43]MIZUTANIM,TSUDAS,SUZUKIK,eta.l.Evaluationofposttranscriptionalgenesilencingmethodsusingflowercolorastheindicator[J].PlantandCellPhysiology,2003,44(Supplement):122.[44]SUZUKIK,ZUEH,TANAKAY,eta.l.FlowercolormodificationsofToreniahybridabycosuppressionofanthocyaninbiosynthesisgenes[J].MolecularBreeding,2000,6(3):239-246.[45]UEYAMAY,SUZUKIK,FUKUCHIMIZUTANIM,eta.l.Molecularandbiochemicalcharacterizationoftoreniaflavonoid3!-hydroxylaseandflavonesynthaseIIandmodificationofflowercolorbymodulatingtheexpressionofthesegenes[J].PlantScience,2002,163(2):253-263.[46]SAVINKW,BAUDINETTESC,GRAHAMMW,eta.l.AntisenseACCoxidaseRNAdelayscarnationpetalsenescence[J].HortScience,1995,30(5):970-972.[47]BOVYAG,ANGENENTGC,DONSHJMeta.l.HeterologousexpressionoftheArabidopsisetr1-1alleleinhibitsthesenescenceofcarnationflowers[J].Molecularbreeding,1999,5(4):301-308.[48]AIDAR,YOSHIDAT,ICHIMURAK,eta.l.ExtensionofflowerlongevityintransgenictoreniaplantsincorporatingACCoxidasetransgene[J].Plantscience,1998,138(1):91-101.[49]COBBD,SCHNEITERN,GUOS,eta.l.Flowerspecificexpressionofanethylenereceptor(ETR1-1)confersethyleneinsensitivityintransgenicpetunia[C]//TheAbstractofXXVIInternationalHorticulturalCongress,Toronto,2002:83.[50]SHAWJF,CHENHH,TSAIMF,et