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1,1,1,2(1.,563002;2.,550025):(SclerotiumrolfsiiSacc.),。:PDA,,;Czapek,,;,,;,10:1;50℃10min55℃5min。:;;:S681.9:A:1004-874X(2010)08-0128-03StudyonthebiologicalcharacteristicofsouthernblightpathogenofAconitumkusenezoffiiReichb.LIXiao-xia1,XIAOZhong-jiu,LIDai1,SANGWei-jun2(1.DepartmentofBiology,ZunyiNormalCollege,Zunyi563002,China;2.AgricultureCollege,GuizhouUniversity,Guiyang550025,China)Abstract:BiologicalcharacteristicsofSclerotiumrolfiiSacc.wastestedbymeasuringmycelialgrowthandproducingsclerotiaindifferentconditions,Theresultsshowedthat:forthegrowthofmyceliaonPDAmedium,12hphotoperiodwasbeneficialtomyceliangrowth,entireilluminationwasbeneficialtoproducingsclerotia.AmongthetestedcarbonandnitrogensourcesinCzapekmedium,inositolandglutamatewerenotgoodforSclerotiumrolfiiSacc.,starchandcalciumnitratewerethemostfavorableforit.Incarbon-nitrogenratiotest,10∶1wasbeneficialtomyceliangrowth.Thelethaltemperatureforthemyceliumwas50℃for10minutesandforthesclerotiumwas55℃for5minutes.Keywords:AconitumkusenezoffiiRelch.;southernblight;biologitmlcharacteristics(AconitumkusenezoffiiRelch.)(),,、、,,、、[1]。,、,。,。,、、,。11.1,PDA[3],。1.21.2.1PDA2d,、5mm(),10mLPDA(75mm),(∶=24h∶0)、(∶=12h∶12h)、(∶=0∶24h)25℃,20W,36cm。3。2d,10d。:PDA20/[4],(∶=24h∶0)、(∶=12h∶12h)、(∶=0∶24h)25℃。3。24h,5d,。1.2.20.2mL,50、5560℃5、10、15min,,PDA,25℃。3。2d。1.2.1,2d。1.2.3Czapek,Czapek200mL,100mL,10min,、、、、、、、8,10min[5]。10mL,,25℃。2d,10d。1.2.1。:2010-02-04:([2003]25):(1979-),,,,E-mail:lixiaoxia825@126.com:(1963-),,,,E-mail:weijunsang@163.com20108128CMYK(/)555aA430aAB154bB(mm/d)19.15aA22.00aA19.85aA(mm)43.349.044.7++++++++++++12h1:(1)“++++”;(2),。(/)15.0aA0.0cC0.0cC10.7bB0.0cC0.0cC0.0cC0.0cC(mm/d)15.2bB14.2bC4.3eF17.8aA15.1bB10.8dE12.5cD10.5dE(mm)60.856.717.271.360.343.250.042.0++++++++++++++++++++++++++++D-3:(1)“++++”,“++”;(2),,4。15min100010min50005min1000015min---10min---5min++--50℃55℃60℃(%)2:(1)“++”,“-”。1.2.4Czapek,Czapek200mL,100mL,10min,、、、、、、L-、L-、L-、、11,10min[5]。10mL,,25℃。2d,10d。1.2.1。1.2.5Czapek,,C∶N0∶1()、10∶1、20∶1、30∶1、45∶1、65∶1、85∶11∶0()8。,,25℃。2d,10d。1.2.1。22.1,22.0mm/d;,154;,555。,(1)。90%,。2.2,50℃10min,55℃5min(2),。2.33,(71.8mm/d),,;(15.2mm/d)、D-(15.1mm/d),、,。,10d,15.0/,10.7/。,,,5d100%(1)。2.4(18.0mm/d),L-(17.9mm/d)、(17.8mm/d)、(12.5mm/d)、(11.0mm/d)、(10.8mm/d)、(9.0mm/d)。,2.1mm/d,(4)。,,。4,,,3。2,,、L-、,5d100%,、、L-。2.5,,、,10∶112.5mm/d(5)。,,。1(%)5d1d3d129CMYK(/)0bB0bB0bB3aA0bB0bB0bB0bB0bB0bB0bB(mm/d)18.0aA17.9aA17.8aA12.5bB10.8bcdBC11.0bcBC9.0cdeCD8.5deCD8.0eD2.1fE0.0fE(mm)72.071.571.050.143.144.235.834.232.08.55.0++++++++++++++++++++++-L-L-L-4:“-”。,5d85∶1,100%(4)。3,。,,,:、;、。,,。(、)、、。:[1],,,.[J].,1995,10(1):54-61.[2],,,.[J].,2007,35(3):760-761.[3].[M].:,1979:122-124.[4],,,.[J].,2003,33(5):406-410.[5].[D].:,2005.2(%)5d1d3d(/)00000000(mm/d)17.5aA12.5bB11.7cBC10.5dD10.6dD10.5dD11.5cBC17.5aA(mm)70.050.046.842.042.342.046.070.0++++++++++++++++++++++10∶120∶130∶145∶165∶185∶15:“+++”。!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!,,,。,,,。,,、。,(MussaendapubescensAit.f.),,,。,,;,,,;,,。,,。。,,。AnnalsofBotany。(%)35d1d3d10∶120∶130∶145∶165∶185∶1130CMYK
本文标题:草乌白绢病菌生物学特性研究
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