长期灌溉含多环芳烃污水对稻田土壤酶活性与微生物种群数量的影响

*张晶1,2张惠文1**丛峰3张勤1,2李新宇1苏振成1张成刚1(1,110016;2,100039;3,110042)以沈抚污水灌区为例,研究了长期灌溉含多环芳烃(PAHs)污水对稻田土壤酶活性微生物种群数量的影响结果表明,灌区稻田土壤PAHs含量在31915~636218Lg#kg-1长期污水灌溉导致稻田土壤PAHs含量严重超过环境标准随清水连续灌溉年限的增加,土壤PAHs总量不同程度降低直至低于土壤PAHs环境质量标准相关性分析表明,在目前污染程度下,灌区稻田3大土壤微生物类群和主要功能群的种群数量主要受土壤理化性质的影响,受PAHs含量影响不明显土壤全氮含量与细菌数量呈极显著正相关(P0101)土壤酶活性受到土壤养分和PAHs污染的双重影响,土壤有机碳和全磷含量分别与脱氢酶多酚氧化酶和脲酶活性呈极显著正相关(P0101),PAHs含量分别与脱氢酶和脲酶活性呈极显著正相关(P0101),与多酚氧化酶活性呈显著正相关(P0105)污灌;多环芳烃;土壤酶活性;微生物种群X172A1000-4890(2007)08-1193-06Effectsoflong2termPAHs2containingwastewaterirrigationonlowlandricesoilenzymeactivitiesandmicrobialpopulations.ZHANGJing1,2,ZHANGHui2wen1,CONGFeng3,ZHANGQin1,2,LIXin2yu1,SUZhen2cheng1,ZHANGCheng2gang1(1InstituteofAppliedEcology,ChineseAcademyofSciences,Shenyang110016,China;2GraduateUniversityofChineseAcademyofSciences,Beijing100039,China;3EnvironmentalProtectionBureauofDadongDistrict,Shenyang110042,China).ChineseJournalofEcology,2007,26(8):1193-1198.Abstract:AcasestudywasconductedintheShenfuirrigationareatoinvestigatetheeffectsoflong2termPAHs2containingwastewaterirrigationontheactivitiesofdehydrogenase,polyphenoloxidaseandureaseandonthemicrobialpopulationsinlowlandricesoi.lTheresultsshowedthatafteralong2termPAHs2containingwastewaterirrigation,thePAHscontentintestsoilrangedfrom31915to636218Lg#kg-1,whichsurpassedtheenvironmentalstandardseriously.Withtheincreasingyearoffreshwaterirrigation,soilPAHscontentdecreasedtodifferentdegrees,uptothelevelbelowenvironmentalstandard.Correlationanalysisindicatedthatthequantitiesofsoilmicrobialandmainfunctionalpopulationsweremainlyaffectedbysoilphysicalandchemicalproperties,andlessaffectedbysoilPAHsconten.tThenumbersofsoilbacteriahadverysignifi2cantlypositivecorrelationswithsoiltotalN,whilethetestenzymeactivitiesweresignificantlypositivelycorrelatedwiththecontentsofsoilorganicC,totalPandPAHs.Keywords:wastewaterirrigation;polycyclicaromatichydrocarbons(PAHs);soilenzymeactiv2ity;microbialpopulation.*(2004CB418503)**E2mai:[e-mail]:2006206219:2007205208ChineseJournalofEcology2007,26(8):1193-11981(PAHs),()PAHs,PAHs,PAHs,PAHs,(,2002;,2003)PAHs(,1997),PAHs(,2004;,2005),PAHs,PAHs,PAHs,PAHsPAHs,2211,8700hm2,,PAHs,,1992,,,21210~20cm20~40cm,3,10,,1Tab.1WastewaterirrigationhistoryofsoilsamplingsitesA41b50c51dN,123b44c42dE50B41b49c34dN,123b41c23dE50,3C41b47c13dN,123b39c46dE40,10D41b47c57dN,123b39c12dE40,10E41b46c35dN,123b39c12dE40,10F41b41c40dN,123b28c36dE20,30;4e,2131mm,10g,50ml,20ml,4000r#min-15min,,20ml20min,4000r#min-15min,,5,,1ml,1/460~100,2mlB(1B1),,1ml(,1995)214(Cmic)(Vanceetal.,1987),(AnalytikJenaMultiN/C3000,)Cmic=Fc/0145,:Cmic;Fc1;,(,1986)215(TTC);;(,1986)11942683311PAHs2,6,pH,PAHs,3,PAHs2Tab.2Soilbasicphysio2chemicalproperties(cm)pH(H2O)(%)(g#kg-1)(g#kg-1)(mg#kg-1)(g#kg-1)(mg#kg-1)(mg#kg-1)A0~206182401343138118416815001788416610711020~407103241915191110886199018414180109102B0~20515935192113411291831510158321859918420~406100321616118110310719601610314195165C0~2061193317919401971271030138621035416020~4061722716517901627211201251018367191D0~2051933812716811011201080133331194711920~4061592715913101697217401300018976157E0~2061493514912711041401260137551357219520~4061783013918401647319701264017477123F0~20610935188177113011616001554201487319220~40616027108152018894169013978126641353PAHs(Lg#kg-1)Tab.3PAHscontentsinthesoilsirrigatedbywastewater(cm)PAHsABCDEF0~203014?1131312?216916?711ND312?216ND7218?29193014?0131016?114418?117611?014811?01389?22917?21312?116418?415418?011413?01614115?121413517?22137511?8133212?171961?4165312?51699413?351980012?391248611?101125218?1534011?111429614?2019121?3912118?9175615?11152019?1194317?7143513?41296719?5832712?51197?2165915?71567?61811417?3277615?211131712?4118017?01357?61658?51310217?3116[a]70719?281234511?12195814?6123411?2143314?4118818?281274215?351340913?10147319?7163614?4163717?3197215?1218[b]57311?311832811?0116418?11193119?3123714?5148111?2517[k]16416?7129214?4111619?1157?013913?3112314?717[a]73612?491951213?261010117?20194614?4185813?71616915?6013[1,2,32cd]11112?171491?0142119?610915?0151317?3103511?1318[a,h]4210?0164816?1118114?414319?016410?1151116?416[g,h,i]9119?4179913?4172610?714917?0151414?2183411?1219EPAHs636218?105211369717?5913127216?2561761019?571479211?4211113018?2061320~4030?8151117?814312?1114?0134?013ND6815?29172114?612712?013217?0164?015412?0167217?141623?1215616?118113?011212?012116?016323?171516417?20113911?8131319?5192019?1132418?815121612?651299518?1934119?61620217?171818513?3826811?131217714?151412916?17153517?8191613?1121511?2181619?31248916?251621218?8154316?4113511?0183318?2164514?11944419?1616220?945?26516?30133014?2124012?017[a]34817?251219611?24132217?4112112?1141813?1142818?01835811?241824519?29192819?2181912?0151912?1192418?119[b]26711?241219818?28142519?3182016?0121819?2162814?312[k]7219?22125117?1415512?016417?012419?017816?018[a]33011?881928512?69113615?6123213?2123013?6105916?411[1,2,32cd]5215?10195312?20131012?217717?011717?1171311?012[a,h]19?11431?814218?012211?014118?111313?017[g,h,i]4718?8166212?22161019?116716?011718?1181315?013E