Illumina微珠基因芯片检测系统

Illumina()2090,(Genechip;DNA,DNAMicroarray),,,[1],,[2],[3],,RNAcDNA,RNA(RNA),cDNA,[4,5],,,,,:2005208220MSCMSCMSC,,,;,,,,,,[9]4[1]PittingerM,MackayA,BeckS,etal.Multilineagepotentialofadulthumanmesenchymalstemcells.Sci2ence,1999,284:143147[2]CongetPA,MinguellJJ.Phenotypicalandfunctionalpropertiesofhumanbonemarrowmesenchymalprogeni2torcells.JCellPhysiol,1999,181:6773[3]DeansRJ,MoseleyAB.Mesenchymalstemcells:biologyandpotentialclinicaluses.ExpHematol,2000,28:875884[4]ZhangY,LiCD,JiangXX,etal.Humanplacenta2derivedmesenchymalprogenitorcellssupportcultureex2pansionoflong2termculture2initiatingcellsfromcordbloodCD34+cells.ExpHematol,2004,32:657664[5]ZhangY,LiCD,JiangXX,etal.Comparisonofmesenchymalstemcellsfromhumanplacentaandbonemarrow.ChinMedJ(Engl),2004,117:882887[6]BarryFP,BoyntonRE,HaynesworthS,etal.ThemonoclonalantibodySH22,raisedagainsthumanmes2enchymalstemcells,recognizesanepitopeonendoglin(CD105).BiochemBiophysResCommun,1999,265:134139[7]BarryF,BoyntonR,MurphyM,etal.TheSH23andSH24antibodiesrecognizedistinctepitopesonCD73fromhumanmesenchymalstemcells.BiochemBiophysResCommun,2001,289:519524[8]BruderSP,JaiswalN,HaynesworthSE.Growthki2netics,selfrenewal,andtheosteogenicpotentialofpuri2fiedhumanmesenchymalstemcellsduringextensivesubcultivationandfollowingcryopreservation.JCellBiochem,1997,64:278294[9]RomanovYA,SvintsitskayaVA,SmirnovVN.Searchingforalternativesourcesofpostnatalhumanmesenchymalstemcells:candidateMSC2Likecellsfromumbilicalcord.StemCells,2003,21:105110200510265JournalofCapitalUniversityofMedicalSciencesOct.2005Vol.26No.5Illumina,RNA(RNA2mediatedanneal2ing,selection,andligation,RASL),100mRNA[6],RASL,IlluminaDASL(cDNA2mediatedannealing,selection,exten2sion,andligation)[7]oligo2d(T)18RNAcDNA,cDNA,PCR,100bp,,,DASL12001500,96DASL,Dhanasekaran[8]212,(PC23),,99%,LaCNP,2944,Mitchell[9],PC23LacNPBeadSta2tion500()RNA,,,Illumina2005920,Max2PlanckBeadStation500,,BeadStation500,,,,,,,,,Illumina[1]LipshutzRJ,FodorSP,GingerasTR,etal.Highdensitysyntheticoligonucleotidearrays.NatGenet,1999,21:2024[2]WelshJB,ZarrinkarPP,SapinosoLM,etal.Analy2sisofgeneexpressionprofilesinnormalandneoplasticovariantissuesamplesidentifiescandidatemolecularmarkersofepithelialovariancancer.ProcNatlAcadSci,2001,98:11761181[3]vantVeerLJ,DaiH,vandeVijverMJ,etal.Geneexpressionprofilingpredictsclinicaloutcomeofbreastcancer.Nature,2002,415:530536[4]GodfreyTE,KimSH,ChaviraM,etal.QuantitativemRNAexpressionanalysisfromformalin2fixed,paraf2fin2embedded.tissuesusing5nucleasequantitativere2versetranscription2polymerasechainreaction.JMolDiagnm2000,2:8491[5]KarstenSL,VanDeerlinVM,SabattiC,etal.Anevaluationoftyramidesignalamplificationandarchivedfixedandfrozentissueinmicroarraygeneexpressionanalysis.NucleicAcidsRes,2002,30:E4[6]YeakleyJM,FanJB,DoucetD,etal.Profilingalter2nativesplicingonfiber2opticarrays.NatBiotechnol,2002,20:353358[7]FanJB,YeakleyJM,BibikovaM,etal.Aversatileassayforhigh2throughputgeneexpressionprofilingonuniversalarraymatrices.GenomeRes,2004,14:878885[8]DhanasekaranSM,BarretteTR,GhoshD,etal.De2lineationofprognosticbiomarkersinprostatecancer.Nature,2001,412:822826[9]MitchellS,AbelP,WareM,etal.Phenotypicandgenotypiccharacterizationofcommonlyusedhumanprostaticcelllines.BritJUrolInt,2000,85:9329441465:Illumina