中华中医药学刊

26620086CHINESEARCHIVESOFTRADITIONALCHINESEMEDICINEVol.26No.6Jun.20081289,(,325027):(BBB),BBB:BBB,,CTMRI,,BBB,,,:;;:R2-03:A:1673-7717(2008)06-1289-04ApplicationandThingkingofResearchMethodsofBlood-BrainBarrierinTraditionalChineseMedicineXULong2long,ZHENGGuo2qing(Neurologycenter,theSecondaffiliatedhospitalofWenzhouMedicalCollege,Wenzhou325027,China)Abstract:Theblood$brainbarrier(BBB)isthespecializedsystem,whichensuresproperfunctionofthecentralnervoussystem.Manymethods,suchasmicroscopyandtracer(Evansblue,lanthanum,radionuclide,etal),imageolo2gy(CT,MRI),invitromodel,severalbiochemicalindicatrixareappliedtoinvestigatethemorphousandfunctionofblood-brainbarrierinwesternmedicine.Tointroducethemainmethodsthatareusedtomeasuretheroleofthetradi2tionalChinesemedicine(TCM)inBBB.TheroleofTCMinBBBwouldbebetterdiscovereddependingonthedevelop2mentofnewmethodologiesofBBB,whichwillbehelpfultoilluminatescientificallythetheoryofTCMandmechanismsofTCMandacupuncture.Keywords:Blood-Brainbarrier;traditionalChinesemedicine;researchmethods:2008-01-11:(1977-),,,,,:(1975-),,,,,(Blood-Brainbarrier,BBB),,:,,3,(JAM),(ZO)-lZO-2ZO-3/,BBB,,,[1-3],,,BBB,BBBBBB,1BBBBBB,,BBB,,,[4][5],,BBB,,982126620086CHINESEARCHIVESOFTRADITIONALCHINESEMEDICINEVol.26No.6Jun.20081290[6]BBB,,BBB,[7],,,,,BBB2125I3H14C13NBBB,BBB2.1,,961,,BBB,BBB,BBB[8],MPIAS500,(Sd/T,S,d,T),[9],,,[10],4h,48h,,,[11],,A,,,,2.2,433,40A,BBBBBB,[12],,,,,2.3125I3H[13]125I,,50%2h,;34h,,125I,,[16]125I,,,[17],3H-BBB,,1.99,3H-()[18]3H-3H-,,,3CTMRI,BBB,BBB,,,CT,,CTBBBCT,,,,,[19][20]BBB,CT,,,CT(),,,CT,MRIT2WI,BBBT1WI,BBB,,,BBB[19][21],T2WIT1WIBBB,1hT2WI,T1WI3h,,T2WIT1WI,T2WIT1WI,MRIBBB,T2WIT1WI4092126620086CHINESEARCHIVESOFTRADITIONALCHINESEMEDICINEVol.26No.6Jun.20081291BBBBBBBBBBBBBBBBBBBBB,,,BBB,BBB[22-23]BBBTranND[24]-,[25],;,5BBB,,BBB,,BBBBBB,,,(1)BBB:[26]BBB,BBB=/,BBB,,BBB(2):,,BBB[27](3)ZO-1ZO-1,[28]ZO-16h,,,ZO-1,,6BBB,BBB,,BBB;,BBB,,CTMRI,BBBBBBBBB,;BBB,,(1):,,,,,,:,,[29],,,,(2):BBB;,,3H-,,;CT,,(3):,,BBB,(4):,,[30],,,,,BBB;,,;(P0.05),,,BBB,BBB,,BBBBBB[1]BallabhP,BraunA,NedergaardM.Theblood-brainbarrier:anoverview:structure,regulation,andclinicalimplications[J].NeurobiolDis,2004,16(1):1-13.[2]PersidskyY,RamirezS.H,HaorahJ,etal.BloodbrainBarrier:StructuralComponentsandFunctionUnderPhysiologicandPath2ologicConditions[J].2006,1(3):223-236.[3],,.[J].,2005,27(2):88-89.[4],,,.CTMRI192126620086CHINESEARCHIVESOFTRADITIONALCHINESEMEDICINEVol.26No.6Jun.20081292[J].,2004,26(21):1920-1923.[5],.[J].,2005,13(5):579-582.[6],,,.-4[J].,2006,26(2):152-156.[7],,,.[J]..2004,26(5):422-424.[8],,,.[J].,2005,45(11):21-22.[9],.[J].,2005,9(25):94-95.[10],,.[J].,2006,35(5):481-483.[10],,.A[J].,2005,18(3):313-314.[12],,,.[J].,2006,14(8):665-668.[13],,,.[J].,2001,20(2):41-43.[14],,,.[J].,2005,26(10):934-937.[15],,,.[J].,2005,7(1):42-45.[16],,,.[J].,1997,23(2):128-130.[17],,,.3H-[J].,1989,4(4):22-24.[18],,,.3H-3H-[J].,1994,25(3):325-328.[19].MR[J].,2002,25(4):221-224.[20],,,.[J].,1994,35(1):46-47.[21],,,.MRI[J].,2005,40(2):235-238.[22]TerasakiT,OhtsukiS.Brain-to-bloodtransportersforendog2enoussubstratesandxenobioticsattheblood-brainbarrier:anoverviewofbiologyandmethodology[J].NeuroRx,2005,2(1):63-72.[23],,,.[J].,2005,22(2):149-151.[24]TranND,CorrealeJ,SchreiberSS,etal.Transforminggrowthfactor-betamediatesastrocyte-specificregulationofbrainen2dothelialanticoagulantfactors[J].Stroke,1999,30(8):1671-1678.[25],.[J].,2004,24(7):632-634[26].[M].:,1998,37:395.[27],,.[J].,2005,5(3):2-3[28],,,.ZO-1[J].,2004,25(5):329-332.[29],,,.[J].,2006,33(9):1087-1088.[30],,,.[J].,2002,23(2):1-4.,TMPRSS6,,,,,,,,,,,,51,TMPRSS6,TMPRSS6,TMPRSS6,Hepcidin,,,,,,TMPRSS6Hepcidin,,,,,2921