中药栀子超临界萃取物的挥发性成分研究

256200611JournalofFoodScienceandBiotechnologyVol.25No.6Nov.2006:167321689(2006)0620087206:2005212218;:2006203215.:(CG20010006).:(19622),,,,.1,1,2,3,1(1.,214036;2.,214036;3.,214062):L9(34),GC2MS:55,35MPa,120min,,,,22,42,,22,42,,:;;;:Q949.781.1;R284.1:AStudyonVolatileCompoundsfromGardeniajasminoidesEllisbySupercriticalFluidExtractionZHANGJia2li1,QIANHua2li1,WANGLi2ping2,XURui2zhen3,TAOWen2yi1(1.KeyLaboratoryofIndustrialBiotechnology,MinistryofEducation,SouthernYangtzeUniversity,Wuxi214036,China;2.Testing&AnalysisCenter,SouthernYangtzeUniversity,Wuxi214036,China;3.WuxiShanhePharma2ceuticalCo.,Ltd.,Wuxi214062,China)Abstract:TostudytheoptimumparametersandcomponentsonvolatileoilfromGardeniajasminoidesEllis.ThesupercriticalfluidextractionforthevolatileoilsinGardeniajasminoidesElliswerestudiedbyorthogonaltest,andtheanalysisofthevolatilecompoundsobtainedfromtheSFEoilandthesteam2distilledoilbythegaschromatography2massspectrometrymethodwasdone.TheSFEoilsconsistoflinoleicacid,palmiticacidandtrans,trans22,42decadienal,butthemaincontentoftheSDoilswastrans,trans22,42decadienal.Theoptimumconditionswerefound:extractiontemperature55,extractionpressure35MPa,extractiontime120minutes.Comparedwiththatofsteamdistillation,ahighextractionyieldandshortprocessingperiodwasachievedbyusingsupercriticalcarbondioxideextraction.Keywords:GardeniajasminoidesEllis;volatilecompounds;supercriticalfluidextraction;steamdistillation(Rubiaceae)(Gardenia)(GardeniajasminoidesEllis),,,[1],()()()(),[2],85,202296[3],(SteamDistillation,SD),(SupercriticalFluidExtraction,SFE),[4]SFE,GC2MS19,4SFE2CO2SDSFE2CO2SD,2(GasChromatography2MassSpectrometry,GC2MS),,,,1HA221250206:;FinniganTraceMS2:Finnigan;:;:;:;FA1004:;AB2042N:Mettler2Tole2do;(GardeniajasminoidesEllis):,;CO2:99%,;:,;:,22.12.1.1SFE2CO2,20,350g1L,3,3,L9(34)2.1.2SD350g20,10,[5]16h(),,011225g,01035%2.2GC2MS2.2.1010500g,,011g/L21212,10mL(011g/L),,21213DB25MS(30m0125mm0125m);;018mL/min;110L();101;250;:60,10/min1404/min220,8/min25021214250;200;EI+;70ev;33450212151L,2,(Willey6NIST98),,80033111,,B,A,CA2B3C1,55,35MPa,120min(2),88251L9(34)Tab.1L9(34)OrthogonalTestandResultsA/B/MPaC/minD/(g/hg)11(45)1(15)1(120)10.413121(45)2(25)2(180)24.390831(45)3(35)3(240)34.119142(55)1(15)2(180)30.756852(55)2(25)3(240)14.148062(55)3(35)1(120)25.056373(65)1(15)3(240)21.061783(65)2(25)1(120)34.469193(65)3(35)2(240)13.9094K18.92302.23169.93858.4705K29.961113.00799.057010.5088K39.440213.08489.32889.3450R1.038110.85320.88152.0383A2B3C1BACA2B3C12Tab.2AnalysisofVarianceFPA0.197320.08960.260.05B25.9900212.996037.300.053C0.135520.06770.190.05D()0.696820.3484:F0105(2,2)=19100312SFE2CO2SD,33SFESDTab.3CompareofvolatileoilfromGardeniajasminoidesEl2lisbySFEandSD/%/hSD0103516SFE2CO2510562313SFE2CO2SDGC2MS,12,44Tab.4ChemicalconstituentsandrelativecontentofvolatileoilfromGardeniajasminoidesEllis/(mg/kg)()1#2#3#4#5#6#7#8#9#SD1Toluene25.4285.1143.0866.1146.5714.5716.0863.6862.743222methylheptane2223.5713.77124.0004.6572.800.6282.48623trans21,22dimethylcyclohexane21,2222222.4572.8862.2573.0861.71424n2Hexanal2.3437.77114.4053.1415.6313.715.02813.2613.281.3145o2xylene20.0860.08620.1140.20020.14320.057622heptenal,[Z][Z]2220.2861.0282.2570.3433.1142.9140.7431.8002.5430.1437Hexanoicacid21.3141.5710.5711.7711.7430.9431.8001.91428Furan,22pentyl22222220.2280.200220.1140.4289trans,trans22,42heptadienal,22,42222220.22820.2280.1432102,62octadiene,2,62dimethyl22,6222,62220.25720.3430.3430.42820.34321132cyclohexen212one,3,5,52trime2thyl20.1141.1431.3140.3431.3141.4000.7141.4281.4570.9431222octenal,[E]2[E]22220.1711.1430.1431.0000.5430.2000.8860.5710.514986:4/(mg/kg)()1#2#3#4#5#6#7#8#9#SD13Nonanal2.3431.1141.0570.3711.3710.9710.3711.2281.2280.37114trans222nonenal222220.60020.6000.514220.457215trans242nonenal24220.5430.97121.2001.20021.1430.114216Octanoicacid20.3140.62821.0000.7710.3140.2860.428217Safranal20.0862220.0572220.228182,42nonadienal2,42220.17120.286220.1430.2860.17119Eucarvone0.8004.9713.2000.8573.8864.7712.9434.1143.5141.42820trans222decenal22220.6571.4860.2572.1431.9710.7141.3713.9430.94321trans,trans22,42decadienal,22,420.91412.3754.637.05761.4849.717.71446.9750.7756.6622Elemene0.6573.7145.4860.2001.4001.3240.7140.8860.6861.42823Selinene220.2862222220.97124Neocurdione1.6002.7430.71422220.17120.57125Curdione28.3739.206.3142.85725.178.8005.5434.4866.0001.62826Germacrone4.1715.7141.5430.0282.8861.2860.7430.5710.1430.68627Myristicacid221.5140.3711.6861.8002.2281.5142.1140.25728Methylpalmitate21.6280.8280.4001.0571.9431.3710.8571.1432.37129Palmiticacid258.80187.929.88187.4182.2212.4167.9205.54.60030Ethylpalmitate21.1431.1430.2861.2861.000221.4280.42831Linoleicacid,methylester1.2003.5713.37163.804.2281.0005.0284.1435.6571.88632Oleicacid,methylester0.7712.6002.8860.5713.5716.8283.8003.0865.2571.57133Ethyllinoleate220.0280.571222220.31434Oleicacid[Z],ethylester220.8002222220.2573522methyloctane22220.5432220.6571.2000.60023632methyloctane3220.2000.40020.4281.0280.4280.3430.17123742methyloctane4222221.3141.40020.8862238Nonane20.5430.71421.4281.3430.4