便携式PCR_CE微流体芯片分析仪控制系统设计

2710200610ChineseJournalofScientificInstrumentVol127No110Oct12006PCR2CE31121(250061)2(250100)DNA(PCR)(CE)32ARMPCR3PCR,PID,PID,PCR,,,PCRARMPIDTP216+.3A510.8040Designofthecontrolsystemforaportablemicro2fluidicchipanalyzerWangHaifeng1ZhangYulin1ZhangHaili21(Theschoolofcontrolscienceandengineering,ShandongUniversity,Jinan250061,China)2(ECEDepartment,ShandongInstituteofLightIndustry,Jinan250100,China)AbstractThecontrolsystemforaportablemicro2fluidicchipanalyzerwasdesigned,whichperformsase2riesofanalyticalstepsrangingfromsamplepre2concentration,PolymeraseChainReactionandCapillaryE2lectrophoresis(PCR2CE)separation.Thecontrolsystembasedonembedded322bitRISCmicrocontrollerARM(LPC2138)meetrequirementsoftheconstanttemperaturepresisecontrolin3temperaturezonesforPCRandhighvoltageelectricalfieldcontrolforCE.Becauseprecisetemperaturecontroliscritical,fuzzyimmunePIDcontrolarithmeticisusedtoensuretheprecisionandrobustnessofthetemperaturecontrol.Themicro2fluidicchipanalyzeralsohasmatrixkeyboard,smallLCDdisplayer,serialcommunicationunit,andcapillaryelectrophoresis(CE)separationunit.TheexperimentresultshowsthattheanalyzerfulfillsalldesignrequirementsandfeaturesprecisePCRtemperaturecontrol,extensiveanalysissoftwarefunctions,highreliability,lowcost,smallsizeandisconvenientforoutdooruse.KeywordsPCRmicro2fluidicchipARMmuzzyimmunePID1,[122]PCR(),DNA,DNA,DNAPCR2CE:,DNA,PCR,3,DNA,320058,(90307003)10:PCR2CE1195DNA,DNAPCR,,,32ARMPCR2CE1,21PCR2CE2PCR2CE2PCR2CEPCRPCR,,DNA:PCR;:2.1,,,32ARM(LPC2138)LPC2138ARM7TDMI2S,CPU60MHz,32KBSRAM,512KBFlash,PWM,10A/D,D/A,46GPIOTQFP64,[3],,c/os2LPC2138,I/O2.23NiMHAA2size414Wh(220V,a.c./9V,d.c.)9VDW1(),78069V6V,MAX713NiMH,MAX713(),DC,DC6V,DC1.5V(2V)MaximMAX170613DC/DC,,33.6V5.5V,5.5V5V,,SPX1117M323.33.3VARMSPX1117M323.3SipexLDO,,,MAX8867,2.56.5V,300mA,2MAX8867(70mA)332.3,LPC21382UART16FIFO,550,UARTPC,3VRS232SP3232E2.4PCR2.4.1331196274,33,A/D(MAX187)(AD620)LPC21382.4.2LPC2138PIDPWM,4L293D032VPWM,42.4.3PIDPID,,,PIDSmith,,T(TlymphocyteT2cell)B(Blympho2cyteB2cell),,TTH(helperT2cell)TS(suppressorT2cell)k()(k),TTH:TH(k)TH(k)=k1(k)(1):k1,TTSB,,BTS(k),:TS(k)=k2f(s(k))(k)(2):k2,f(),TS,[0,1]B:s(k)=TH(k)-TS(k)(3)(3)PID:uPID(k)=u(k-1)+kp{e(k)-e(k-1)+kikpe(k)+kdkp[e(k)-2e(k-1)+e(k-2)]}(4):kpkikdP:u(k)=kpe(k)(5),ke(k)k(k),ku(k)kBs(k):u(k)=k1[1-f(u(k),u(k))]e(k)=kpe(k)(6):k1,k1,;=k2/k1,,,10:PCR2CE1197k1,f()TSf[u(k),u(k)],u(k)u(k),P,kp,P,PID,PIDuPID(k)()(k),PID[4]:u(k)=u(k-1)+kp[e(k)-e(k-1)+kikpe(k)+kdkp(e(k)-2e(k-1)+e(k-2))](7):kp=k1[1-f(u(k),u(k))]f(),,u(k)u(k),PBNB;f(),NBZPB,f():IfuisPBanduisPBthenf(u,u)isNBIfuisPBanduisNBthenf(u,u)isZIfuisNBanduisPBthenf(u,u)isZIfuisNBanduisNBthenf(u,u)isPB.[425],:G(s)=k1TS+1e-s(8):k;,;T:k=1T=0.75=5PCR,1Matlab,1s,PID:=0.850k=0.30ki=0.30kd=05(rin/rout/,,)PID,70,(55)6,5,MatlabCOMbuilderPIDCOMVCPIDPWMARM,I/O3955575,60s,,5/s,0.5,,0.5,PCR,77PCR2.5PCR2CEPCRCE,PCR,,PCB,,v,,E,:v=E(9)119827PWM,,LPC2138DNA,,2.6,PCR,,EMCO-0.614,10000V,PCR2CEEMCOPWMDAC,,EMCOPWM,,,2,1,,CE,1,2,CE,,,,883,PCRVC,,Ct,3[6]:(l),PCR;(2),PCR,;(3),PCR,,,,,,PCR,:ctccycle,tthreshold,ct:ct,,ct9,,,ctct,9ctPCR(),,,DNA(DNA),,,,10,PCR,DNATNF2a,5,10:PCR2CE1199104PCR2CEPCR,CE,,DNA(PCR)(CE),,PCR,,,[1],,,.PCR[J].(),2003,24(4):5432545.[2],.PCRCE[J].(),2003,24(4):2012202.[3],.ARM72LPC213x/LPC214x.[M].:,2005.[4]DINGY,RENL.Fuzzyself2tuningimmunefeedbackcontrollerfortissuehyperthermia[J].FuzzySystems,2000.FUZZIEEE2000.TheNinthIEEEInterna2tionalConference,2000,1:5342538.[5].PIDMATLAB[M].:,2004.[6],.PCR[J].,2004,20(2):1152116,44.,,19787,,E2mail:[e-mail]