四环素类药物分析

HPLCApplicationsDikmaTechnologies1四环素类药物分析序列号：S004色谱柱：SpursilC18，250×4.6mm，5μm流动相A：甲醇+乙腈(50+50)流动相B：0.01mol/L草酸水溶液流速：1.0mL/min柱温：35℃检测器：UV365nm样品：化合物溶于30%甲醇水溶液进样量：20μL梯度设置024681012Time(min)1231土霉素2四环素3金霉素t/min0101120A/%30503030B/%70507070HPLCApplicationsDikmaTechnologies2AnalysisofTetracyclineAntibioticsSerialNumber:S004Column:SpursilC18,250×4.6mm,5μmMobilePhaseA:Methanol+Acetonitrile(1+1)MobilePhaseB:0.10mol/LoxalicacidinwaterFlowRate:1.0mL/minColumnTemperature:35℃Detector:UV365nmSample:Compoundsweredissolvedin30%methanolwatersolutonInjectionVolume:20μLGradientProgram:024681012Time(min)1231Terramycin2Tetracycline3Aureomycint/min0101120A/%30503030B/%70507070