常见的通用引物序列

奥科公司测序部通用引物合成引物名称引物序列T7TAATACGACTCACTATAGGGSP65'ATTTAGGTGACACTATAG3'M13F5'TGTAAAACGACGGCCAGT3'M13R5'CAGGAAACAGCTATGACC3'T7（17BASE）5'ACATCCACTTTGCCTTTCTC3'T7TER5'TGCTAGTTATTGCTCAGCGG3'S.TAG5'CGAACGCCAGCACATGGACA3'T35'ATTAACCCTCACTAAAGGGA3'M13F（47）5'CGCCAGGGTTTTCCCAGTCACGAC3'M13R（48）5'AGCGGATAACAATTTCACACAGGA3'PGEX5’5'GGGCTGGCAAGCCACGTTTGGTG3'PGEX3’5'CCGGGAGCTGCATGTGTCAGAGG3'PEGFPC5’5'CATGGTCCTGCTGGAGTTCGTG3'PEGFPC3’5'TATGGCTGATTATGATCAGT3'PEGFPN5’5'TGGGAGGTCTATATAAGCAGAG3'PEGFPN3’5'CGTCGCCGTCCAGCTCGACCAG3'PBV220F5'AAGAAGGGCAGCATTCAAAG3'PBV220R5'CTGCGTTCTGATTTAATCTG3'5’AOX5'GACTGGTTCCAATTGACAAGC3'3’AOX5'GGCAAATGGCATTCTGACAT3'AFACTOR5'TACTATTGCCAGCATTGCTGC3'M13(96)5'CCCTCATAGTTAGCGTAACG3'PCMVF-BD5'TCTAAAAGCTGCGGAATTGT3'PCMVR5'TCCAAACTCATCAATGTATC3'P171105’TACCACTACAATGGATG3’P125845’TTTTCAGTATCTACGAT3’PAS21F5'TCATCGGAAGCGAGTAG3'PAS21R5'CGTTTTAAAACCTAAGAGTCAC3'3'AD5'AGATGGTGCACGATGCACAG3'3'BD5'TAAGAGTCACTTTAAAATTTGTATC3'PQE30F5'TGAGCGGATAACAATTTCAC3'PQE30R5'GTTCTGAGGTCATTACTGG3'S15’CAACGTGAAAAAATTATTATTCGC3’S65’GTAAATGAATTTTCTGTATGAGG3’GLP15'TGTATCTTATGGTACTGTAACTG3'GLP25'CTTTATGTTTTTGGCGTCTTCCA3'RVP35'CTAGCAAAATAGGCTGTCCC3'RVP45'GACGATAGTCATGCCCCGCG3'PTRC99CF5’TTGCGCCGACATCATAAC3’PTRC99CR5’CTGCGTTCTGATTTAATCTG3’PIRES2EGFPF5'GTAGGCGTGTACGGTGGGAG3'PIRES3EGFPR5'AACGCACACCGGCCTTATTC3'PCDNA3.0F25'CTCTGGCTAACTAGAGAACC3'PCDNA3.0R25'GGCAACTAGAAGGCACAGTC3'BAC15'AACCATCTCGCAAATAAATA3'BAC25'ACGCACAGAATCTAGCGCTT3'PB42ADF5'CCAGCCTCTTGCTGAGTGGAGATG3'PB42ADR5'AAGCCGACAACCTTGATTGGAG3'TRXF5'CATATGAGCGATAAAATTATTCAC3'TRXR5'GGATCCCTTGTCATCGTCATCACCA3'pTARGETSEQ5’TTACGCCAAGTTATTTAGGTGACA3’pMALC2XF5’TGCGTACTGCGGTGATCAAC3’pMALC2XR5’CTGCAAGGCGATTAAGTTGG3’pbdgal4f5'GCCTCTAACATTGAGACAGC3'pbdgal4r5'AAGAGTTACTCAAGAACAAGAA3'pbi121(35s)5'GACGCACAATCCCACTATCC3'PEFF5'TCAAGCCTCAGACAGTGGTTC3'PCEPF5'AGAGCTCGTTTAGTGAACCG3'EBVR5'GTGGTTTGTCCAAACTCATC3'CMVF5'CGCAAATGGGCGGTAGGCGTG3'CMVR5'GTTCACGGTGCCCTCC3'PTRCHISAF5’CATGGTATGGCTAGCATGAC3’PTRCHISAR5’CAGGCTGAAAATCTTCTCTC3’pDC316F5'-TAACTTGTTTATTGCAGC-3'pDC316A5'-TATGCTTACCGTAACTTG-3'BGH5'TAGAAGGCACAGTCGAGG3'pDC316F5'acgtgggtataagaggcg3'pDC316R5'cgatgctagacgatccag3'pshuttle-cmvF5'-ggTCTATATAAgCAgAgCTg-3'pshuttle-cmvR5'-gTggTATggCTgATTATgATCAg-3'GAL4BD5'TCATCGGAAGAGAGTAG3'TADH5'GTAAATTTCTGGCAAGGTAGAC3'pDsRed-n5'GACTTTCCAAAATGTCGTAAT7-EEV5'AAGGCTAGAGTACTTAATACGA3'pfastBac-5'GTCCGAAACCATGTCGTACpfastBac-3'CTGATTATGATCCTCTAGTACTTCPBAD5'TATCGCAACTCTCTACTGTTPBAD3'5'AGCCAAGCTGGAGACCGTTT3'5'ADAATACCACTACAATGGATGATGN-CMV-30ATAACCCCGCCCCGTTGC-CMV-24TTAGGACAAGGCTGGTGGpBABE_5'ctttatccagccctcacpBABE_3'accctaactgacacacattccP5'TGCGTACTGCGGTGATCAACP3'CTGCAAGGCGATTAAGTTGGDuetDOWN15'-GATTATGCGGCCGTGTACAA-3'PETUpstreamATGCGTCCGGCGTAGAGGATDuetUP25'-TTGTACACGGCCGCATAATC-3'pSR5'PrimerggaagccttggcttttgSV40PolyA5'TAAGATACATTGATGAGTTTGGAC3'pCMV5RATTATAgAggACACCTAgTCpCMV5FTTCCAAAATgTCgTAATAAC