止血中草药断血流的研究进展

1,2,1,2(1.,210009;2.,221007):,,:;;;1(HerbaClinopodii)Clinopodi2umchenensis(Benth.)O.Kuntze()Cli2nopodiumpolycephalum(Vaniot)C.Y.WuetHsuan(1406),(1848),;2005[1]2,,[2,3]2.12.1.1(dydimin)(hesperidin)272(isosakuranetin272rutinoside)(apigenin)(1elereoin)272(nairutin)(prunin)[4]2.1.2(clinop2odiside)BCDEFG[5,6],(clinopodiside)A2.1.30.13%,44,2.1.4,2;2.22.2.1A2,80%(EtOH),EtOH,(10kg1.2kg),50,,EtOAc,n2BuOH,n2BuOH,,10.8g,10.8g(H500g),CHCl32MeOH2H2O(730.3),100ml,,2028,H200g,CHCl32EtOAc2MeOH(212),MeOHA[7](10kg600mgA)2.2.2,,,,D101,30%70%3BV(BV)1BV/h,70%,70%86.8%[8]3,,2005,b(:A),3.1,,HPLC[9],20053.2A,(HPLC),[10],DISCOVERYC18(4.6mm250mm5m),2(7327),0.8mlmin-1,250nmA15.039.0mmL-1,98.5%,RSD1.3%(n=9),HPLCA2005,,,A0.03%0.07%,,,44.1,,,,,,[11]4.24.2.1(454AnhuiMedicalandPharmaceuticalJournal2007May;11(5)©1994-2008ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.)(1),,,P0.01,(P0.01)(2)6d(),()(P0.01),()(P0.01),(P0.05),:183.057.35s,126.046.7s,165.051.47s,20mgkg-140mgkg-1(6.6713.33),,,10mgkg-15mgkg-1(3.341.67)(PRT);10mgkg-1(SPT)(KPTT),,,,(3)8,20%,0.5ml,20min,F,,244,0.8%,0.1ml,F,,78.6613.04mmHg,11814%,,,,,4.2.2,,48h,,1101204.2.3,,[12],IgG4.2.40.0750.15gL-1,1052.5mgkg-1,,,4.2.51.2gkg-1604.375%,50%,LD5020gkg-127%50%,,27%,50%LD5020gkg-1,LD50(14.62.4)gkg-10.6gkg-1,3.6gkg-,,20100mgkg-1,120mgkg-1,570,,,,5.1100,95,510070,30[13](),1224h[14][15]4939,2045.2,[16]5.3(),[17]5.45.5,,6,,,,,(1)554AnhuiMedicalandPharmaceuticalJournal2007May;11(5)©1994-2008ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(,312000):;;,,(ADR),,,11.1,ADRADR,,,,,,,ADR[1]1.2,(),,,,[2];,ADR,,ADR;,,,,1.3,,1.4:(1)ADR,;(2)pH(3),22.1,[2]413ADR,ADRADR37,9%,ADR,ADR,ADR,ADR,,,;(2),GAP;(3),,;(4):12,,,;(5):[1].[S],.:,2005:228.[2],.[J].,1999,13(3):48-50.[3],.[J].,2002,25(5):342.[4]KuoYH,LeeSHM,LaiJsH.ChemicalstudyofC.gracile[J].ChinPharmJ.(TaiPei),2000,52(1):27-34.[5]LiuZM,LiD,OwenNL,etal.TwotriterpenoidsaponinsfromC.chi2nesis[J].NatProdLett,1995,6(2):157-61.[6]LiuZM,LiD,OwenNL,etal.OleananetriterpenoidsaponinsfromthechinesemedicalherbC.chinensis[J].JNatProd,1995,58(10):1600-4.[7],,,.[J].,1992,27(3):207-12.[8],,.[J].,2005,28(10):942-5.[9],,.[J].,2005,40(4):317-8.[10],,.HPLCA[J].,2006,22(3):217-9.[11],,,.[J].,2005,9(7):486-8.[12],,,.[J].,2005,9(6):413-5.[13].100[J].,2004,25(1):36-8.[14],,,.[J].,2002,417(5):311-3.[15].60[J].,2005,(5):476-7.[16],,.[J].,1996,8(2):49-51.[17].[J].,1996,17(2):46-8.(:2006-12-25)654AnhuiMedicalandPharmaceuticalJournal2007May;11(5)©1994-2008ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.