牛源木糖葡萄球菌的生物学特性及耐药性分析

:20091019:(1983),,,;email:[e-mail]:(1958),,,285201010()JournalofShiheziUniversity(NaturalScience)Vol.28No.5Oct.2010:10077383(2010)05057204,,,,(,832003):,60:,,8,5(100%)(93.3%)(90%)(90%),(43.3%)(46.6%):;;:S852.611:ABiologicalCharacteristicsandMedicineSensitiveTestofStaphyloccusxylosusWEILuyu,YANGenqiang,QIYaying,MOMing,WANGJingmei(CollegeofAnimalScienceandTechnology,ShiheziUniversity,Shihezi832003,China)Abstract:TodeterminepartsofbiolgicalcharacteristicsandmedicinesensitivityofStaphyloccusxylosus,60strainStaphyloccusxylosuswereexaminedwithcommonmethods.Resultsshowedthatexaminedbacteralstrainwerestaphylocoagulasefeminine,catalaseandMannitolfermentationweremasculine.8bacteralstrainshadhemolysisand5bacteralstrainshadpathogenicity.ThedrugsensitivitytestsshowedthatthosestrainswereresistanttoSulfadiazine(drugfastrate100%),benzylpenicillinpotassium(drugfastrate93.3%),Tetracycline(drugfastrate90%),Gentamicin(drugfastrate90%),andmediumsensitivetoCiprofloxacin(drugfastrate43.3%),Ofloxacinmedium(drugfastrate46.6%).Keywords:Staphyloccusxylosus;isolationandidentification;resistancetest(Staphylococcusxylose,S.xylose)(Coagulasenegativestaphylococcus,CNS),[1]CNS,[2],,,,[3],,[4],,,,[4],,,[5],,,12,11.11.1.114623,172,60,1.1.21.1.31.21.2.1BairdParker:10.0g,5.0g,1.0g,10.0g,12.0g,(6)5.0g,20.0g,950mL,45~50,pH7.00.2,12115min,4[6]1.2.225mL,225mL7.5%,3724h,BairdParker,3724h,,[7]1.2.3,(5g/)(0.04IU/),CNS[8],1.2.412120min,50,,5%BairdParker(),,37,24h,1.2.50.5mL0.5mL24h,37,0.5h1,6h,,,,1.2.613%,30s,1.2.7,3724h,1.2.815,2,86,15~20g/,0.2mL(5.0!107CFU),1,,1.2.9KB[9].LB,3716~18h,90mmMH,2(6),15mm,24mm,3718~24h,NCCLS2004[10]22.11,,,5735,:,,,1(100!10)Fig.1StaphylococcusGramstaining(100!10)2.2,24h2~3mm2.3,,,,CNS:,VP,,APIStaph(),,98%2.4,;;2.5824h,,;48h,5,6,2,,2.61260,11,,,,,,112Tab.1Theresultsofresistancetestof12antibioticstoStaphylococcusxylosus/(g/)/mm/%15∀2314~22#134676.730∀2314~22#13610.030∀1715~16#1446.710∀1512~14#115286.75∀2016~20#152643.310∀1513~14#125490.02∀2115~20#141016.630∀1915~18#145490.05∀1613~15#122846.630∀1511~14#101016.6250~300∀1612~14#106010010∀4727~46#265693.33,50%34.88%,,,,,;,,,,[11],,,,,,574()28:[1]RuppPE,ArcherGL.Coagulatenegativestaphylococci:pathogensassociatedwithmedicalprogress[J].ClinInfectDis,1994,19(6):231.[2],.1[J].,1991,14,63.[3]MartindeNicolsMM,VindelA,SaczNietoJA.Epidemiologicaltypingofclinicallysignificantstrainsofcoagulasenegativestaphylococci[J].JHospInfect,1995,29(11):35.[4]HarmonRJ,LangloisBE.MastitisduetocoagulatenegativeStaphylococcusspecies[J].AgriPractice,1989,10:2934.[5],,.[J].:,36(11):4045.[6],.[M].2.:,1997.[7],.[J].:,2009,27(6):5659.[8],,,.()[M].:,2000.[9].[M].:,2002.[10]NCCLS.Performancestandardsforantimicrobialsusceptibilitytesting,fourteenthinformationalsupplement[J].NCCLS,Wayne,Pennsylvania,2004,24(1):151159.[11]KaarinaSH,OlaviE.Efficacyofparenteraladministrationofthreeantimicrobialagentintreatmentofclinicalmastitisinlactatingcows:487(19891995)[J].JAmVetMedAssoc,1998,212(3):407412.5755,: