芦笋茎枯病菌对甲基托布津的抗药性初步研究

(,210095)6812%8819%,(5EC5020LgömL)(20EC50100LgömL)(EC50100LgömL)8,,;;;S436144S64416PreliminarystudyonresistanceofPhomapsisasparagiSacc.tothiophanate-methylChenXinjianandYeZhongyin(KeyLabofMonitoringandManagementofPlantDiseasesandInsects,MinistryofAgriculature,NanjingAgricUniv,Nanjing210095)ABSTRACTThesamplesfromfieldpathogenpopulationofPhomapsisasparagiSacc.inFengxianandGanyu,JiangsuProvincewerecollectedandtheirresistancetothiophanate2methyl(TM)wastestedinvitro1Theresultsshowedthat8819%fieldisolatesfromGanyu,and6812%isolatesfromFengxianwereresistant.ThreelevelsofresistancetoTMwereidentifiedthatislowerresistance(LR)(5EC5020LgömL),moderateresistance(MR)(20EC50100LgömL)andhighresistace(HR)(EC50100LgömL).TheresistancetoTMwasstablyinheritedaftercontinouslytransfered8genera2tionsontheTM2freePDAmedium.HRisolatesshowedpositivecross2resistancetobenomyl,carbendazim,thiabendazoleandsensitivitytodiethofencarb.However,thesensitivitytodiethofencarbwasnotfoundintheLRandMRisolates.Sporu2lationandmyceliagrowthofresistantisolatesweresimilartowild2typeisolates.KeywordsPhomapsisasparagiSacc.;thiophanate2methyl;resistance(PhompsisasparagiSacc.)[1,2],,,,11119316%()9615%()9815%()8512%()112,PDA:(ZL)(SL)(HD)(NJ)(JX)(ZJ)(GY):19982052051999,22(1):2932JournalofNanjingAgriculturalUniversity113PDC3d,5mm,,PDA253d,,2,EC50114,PDA,,011,1,10,100,1000LgömLPDA(5mm),,3,3d,,EC50115,,,,116[4]22111,EC505,520,20100,100LgömL1Table1SensitivityofP.asparagiSacc.isolatestothiophanate-methy1invitroIsolateSensitivityEC50öLgmL21IsolateSensitivityEC50öLgmL21SL17S5HD07MR20100JX02S5GY02MR20100HD01LR520ZJ32HR100ZJ27LR520GY17HR1002121,66,6812%,1812%,2412%,2518%;8819%,1815%,4216%,2718%,213EC50ZL08ZL38HD17HD18PDA,8,PDA,EC50(2),1Fig11SensitivityandresistanceofP1asparagiSacc1tothiophanate2methylinFenxianandGanyuofJiang2suProvince03222EC50Table2EC50ofthiophanate-methyltoresistantstrainsP.asparagiSacc.aftercontinouslytransferedIsolateCyclesofsubcultures2468IsolateCyclesofsubcultures2468HD18602127427199478110319148ZL38409146242159188141184187ZL081042130672158952197765101HD17540138427191351106507167214ZL09ZL12HD23PDAZL09ZL12HD23EC50(3),3PDATable3ResistanceofP.asparagiSacc.onPDAcontainingcarbendazim,thiophanate-methyl,thiabendazoleandbenomylFungicideIsolateEC50öLgmL-1FungicideIsolateEC50öLgmL-1CarbendazimZL09ZL12HD23132130151130902135BenomylZL09ZL12HD23691891241441091130Thiophanate2methylZL09ZL12HD23190132113120928129ThiabendazoleZL09ZL12HD231616664124271155215,[1,3,5],4:HD01HD08GY02EC502395LgömL,HD18GY17ZL23EC5012LgömL,4Table4SensitivityofP.asparagiSacc.isolatestodiethofencarbIsolateTMphenotypeToxiclinearregressiveequation(r)RelativecoefficientEC50öLgmL-1JX01SY=4108+0157X01987741112HD01LRY=4121+0158X01992923102HD08MRY=4132+0139X01987056150GY02MRY=4107+0148X01997892122HD18HRY=3195+8113X01973811345GY7HRY=4143+7168X01992211187ZL32HRY=4103+6164X019994113992165,115213g,,131:5Table5SporationandmyceliagrowthofP.asparagiSacc.isolatesIsolateTMphenotypeögMyceliadryweightöõmL-1ConcentrationofsporsuspensionIsolateTMphenotypeögMyceliadryweightöõmL-1ConcentrationofsporsuspensionHD18HR118321183106HD01LR114761132106HD17HR117841165106JX02S212602154106ZL28MR115491168106SL17S1176211721063,,,,B2198200,Katan&Koerraadt[7,9],198,200,,,,,,,,666812%,548819%,,,,Koerraadt,Ishii&Katan[79]Venturiainaequalis,Venturianashicola,Botrytiscinerea,3,,,,,,,,;,,1,1.,1995,(5):48492,,..,1995,7(6):4574633,,..,,,.:,1991.31404,,..,1987,4():1281345ShabiE,KatanT,MantonK.InheritanceofresistancetobenomylinisolatesofVenturiainaequalisfromIarael.PlantPathololgy,1983,32:2072126YardenO,KantanT.Mutationsleadingtosubstitutionsataminoacids198and200ofbeta2tubulinthatcorrelatewithbenomylresistancephenotypesoffieldstrainsofBotrytiscinerea.Phytopathology,1993,83:147814837KoerraadtH,SomervilleSC,JonesAL.Characterizationofmutationsinthebeta2tublingeneofbenomyl2resistantfieldstrainsofVenturiainaequalisandotherplantpathogenicfungi.Phytopathology,1992,82:134813548IshiiH,VanRaakM.InheritanceofincreasedsensitivitytoN2phenylcarbamatesinbenzimidazole2resistantVenturianashicola.Phytopathology,1988,78:6956989KatanT,YunisH.Resistancetodiethofencarb(NPC)inbenomyl2resistantfieldisolatesofBotrytiscinerea.PlantPathol,1989,38:8692()2322