防治褐飞虱的高毒农药替代药剂的室内筛选及交互抗性研究

1,211,311111(1,210095;2,310021;3,E2mail:[e-mail])LaboratoryScreeningandCross2ResistanceAnalysisofAlternativeInsecticidesforHighly2ToxicPesticidesforControllingBrownPlanthopper,NilaparvatalugensWANGYan2hua1,2,CHENJin1,SHENJin2liang1,3,GAOCong2fen1,HUANGYue1,ZHANGJiu2shuang1,LIWen2hong1,ZHOUWei2jun1(1KeyLaboratoryofMonitoringandManagementofPlantDiseasesandInsects,MinistryofAgriculture,NanjingAgriculturalUniversi2ty,Nanjing210095,China;2InstituteofQualityandStandardforAgro2Products,ZhejiangAcademyofAgriculturalSciences,Hang2zhou310021,China;3Correspondingauthor,E2mail:[e-mail])Abstract:Toscreenalternativeinsecticidesforhighly2toxicpesticides,toxicitiesofmorethantwentyinsecticidesfalleninsixgroupstothe3rdinstarnymphsofbrownplanthopper,NilaparvatalugenspopulationscollectedfromNanningandGuilinCities(GuangxiZhuangAutonomousRegion),ChangdeCity(HunanProvince)andNanjingCity(JiangsuProvince),Chinawereinvestigatedbythericestem2dippingmethodinlaboratoryfrom2005to2006.Resultsshowedthatbuprofezin,fipronil,thiamethoxam,nitenpyram,chlopyrifos,isoprocarb,promecarbandcarbosulfancouldbeadoptedaspotentialalternativeinsecticidesforthehighly2toxicpesticides.Afterselectionwithimidaclopridfor23generations,thepopulationdevelopedextremely2highlevelresistancetoimidacloprid(1298.52fold),withaLC50valueof103.88mg/L,being6.52foldofthevalueoftheoriginalreferencestrainbeforetheselection(16.01mg/L).TheselectedstrainofN.lugensshowedobviouscross2resistancetoimidaclothiz,thiaclopridandacetamiprid,whereasnottodinotefuran,thiamethoxamandnitenpyram.Managementstrategyofinsecticideresistanceoftheinsectwasalsodiscussed.Keywords:Nilaparvatalugens;highly2toxicinsecticide;imidacloprid;cross2resistance;insecticideresistance:,2005-2006,620,,8,123,200.11298.5(5.5);,:;;;;:S435.112+.3;S481.4;S482.3:A:100127216(2008)0520519208Nilaparvatalugens(StÜal)[1],,[2]r2,,[3]1969[4],[528]5,,,[9],,[10](2005270),,:2007211202;:2008202215:[2005()7];;(0200738):(1979-),,915(ChineseJRiceSci),2008,22(5):519526©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(,)(1)(NN)(GL),(CD)(NJ)800500600,(57cm57cm92cm),63,2630,F1F231995,6319932,,120,[11]LC50(LC5016.01mg/L),[11]23,1.2:98.1%():95.3%97%();95%95%();97.7%10%();97.75%():87%():90%96.4%89%93%():99.7%98.5%98%98.5%96%99%();98.5%();88.89%():95%();73%98%();9811%();95%();95%(),10%Triton2100,1.3[11](LC50)SAS[9]72h;96h;1Table1.OriginofNilaparvatalugenspopulations.PopulationOriginYearFieldgeneration&stageGL05Guilin,Guangxi20054,The4thgeneration,eggmassNN05Nanning,Guangxi20054,The4thgeneration,nymphsCD05Changde,Hunan20053,The3rdgeneration,adultsNJ05Nanjing,Jiangsu20052,The2ndgeneration,adultsNN06Nanning,Guangxi20062,The2ndgeneration,adultsNJ06Nanjing,Jiangsu20062,The2ndgeneration,adults025(ChineseJRiceSci)225(20089)©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(RR)=LC50/LC50,LC5095%:3.0;3.15.0;5.110.0;10.140.0;40.1160.0;160.1[9]1.4[11]100034d,63,40%70%(3LC50),,;,,[12]1.5,,22.1662005-2006,6206(25),82.1.12005(GL05)(6),GL0579.1,6GL05:(2)22120053Table2.Toxicitiesof21insecticidestothe3rdinstarnymphsofthepopulationGL05ofN.lugens.InsecticideNo.ofinsectsLD2PlineLC50(95%)LC50(95%confidenceinterval)/(mgL-1)Buprofezin420y=6.2688+2.1386x0.26(0.210.30)Fipronil420y=6.3199+2.5610x0.31(0.260.36)Nitenpyram420y=5.7807+1.7343x0.35(0.290.43)Thiamethoxam420y=4.4980+1.5188x2.14(1.722.69)Chlopyrifos360y=3.6446+2.1538x4.26(3.565.23)Imidacloprid420y=3.5938+1.7548x6.33(4.977.80)Silafluofen420y=3.7751+1.2356x9.80(7.3312.72)Carbosulfan420y=2.5344+2.1633x13.80(11.6316.35)Isoprocarb420y=2.4968+2.1702x14.24(11.9717.23)Promecarb360y=2.6805+1.7909x19.73(15.9225.71)Acetamiprid360y=2.6091+1.7865x21.79(17.9426.88)Fenobucarb420y=1.7616+2.3318x24.48(20.7329.10)Metalcarb420y=1.3553+2.5280x27.65(23.3632.69)Propocarb360y=1.4098+2.2110x45.05(34.9252.53)Dimethacarb420y=1.1052+2.3080x48.70(41.3457.96)Triazophos360y=2.0090+1.7097x56.16(45.8768.73)Methamidophos420y=1.5843+1.8810x65.45(54.3179.21)Dimethoate360y=1.5184+1.5084x203.30(162.37263.01)Cycloprothrin420y=-0.3159+1.7409x1131.19(914.211459.88)Malathion420y=0.3931+1.4958x1201.96(940.501634.91)Fenoxycarb360y=-0.6141+2.8333x9582.31(8298.5911087.29)LC5095%Nonoverlappingbetweenthe95%confidenceintervalsisconsideredasthecriterionforstatisticalsignificance.Thesameasintablesbelow.125:©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(95%)LC50(95%confidenceinterval)/(mgL-1)2006LD2PlineLC50(95%)LC50(95%confidenceinterval)/(mgL-1)Fipronily=7.2623+2.7111x0.11(0.090.13)y=6.4950+2.3018x0.22(0.180.29)Buprofeziny=6.5052+2.1131x0.19(0.140.24)y=6.4334+2.7920x0.31(0.260.35)Nitenpyramy=5.2787+1.6012x0.67(0.550.82)y=6.9447+2.8005x0.20(0.130.27)Thiamethoxamy=5.1292+2.0683x0.87(0.711.04)y=5.3010+2.2368x0.73(0.581.29)Chlopyrifos