非生物学药物筛选方法及其应用

2010-01-232010-05-28No．30771590No．200704204431983-．*1971-．*730050、、、4。、。DOIdoi10．3969/j．issn．1008-0805．2010．10．116R282．7A1008-0805201010-2671-03Non－biologicalDrugScreeningMethodsanditsApplicationsLIUYu-rongLIJian-yong*YANGYa-junKeyLabofNewAnimalDrugProjectLanzhouInstituteofAnimalScienceandVeterinaryPharmaceuticalsChineseAcademyofAgriculturalSciencesLanzhou730050ChinaAbstractMolecularimprintingtechnolgyhigh－throughputscreeningtechnologybiochiptechnologyandbioinformaticstech-nologywerethecommonnon－biologicaldrugscreeningtechnologies．Theconceptsprinciplesandapplicationsforalltechnolo-giesweredescribedinthispaperinordertoapplythesetechnologieswidelyinmoredifficultscreeningfieldsuchasanti－virusandotherdrugs．KeywordsDrugscreeningMolecularimprintingtechnologyHigh－throughputscreeningtechnologiesBiochiptech-nologyBioinformaticstechnology。。。。。。、。1。1molecularimprintingtechniqueMIT。molecularlyimprintedpoly-mersMIPs、、。23、45、6、、7、8、9MIPs。MIPs①－②、－③“”。10。、。111。MIT、、、、、、、MIPs。。。。。12。。。。。2highthroughputscreeningHTS2080。·1762·LISHIZHENMEDICINEANDMATERIAMEDICARESEARCH2010VOL．21NO．1020102110。、、、。13、、。、。。3。。－。。。。。、、G－GPCR13。iiiiiia．b．c．d．1。。。。1415。16PTP1B1B179402．85%17。、218、、。、、、。。19。20NANA12。。。3biochip、、、、、21、、DNA、3、、、、、、、、、22。、、、。、、。。。、、、、、、、23。24。LiX25、。。。。。4、、、、、、。26。。·2762·20102110LISHIZHENMEDICINEANDMATERIAMEDICARESEARCH2010VOL．21NO．10。。、。。、、、、2728。2Swiss－PdbViewerN1a。。。BaoYG29Rv3871、。Rv3871。PhilippeBernard30A2。、。。2N1a。31。BirnbaumerGM32。。。1．J．200819122901．2KandimallaVBJuH．MolecularimprintingadynamictechniquefordiverseapplicationsinanalyticalchemistryJ．AnalBioanalChem20043804587．3AnderssonLI．MolecularimprintingdevelopmentsandapplicationsintheanalyticalchemistryfieldJ．JChromatogrB200074513．4WeissRMolinelliAJakuschMetal．Molecularimprintingandsol-idphaseextractionofflavonoidcompoundsJ．Bioseparation2001106379．5AnderssonLI．Molecularimprintingfordrugbioanalysis．Areviewontheapplicationofimprintedpolymerstosolid－phaseextractionandbindingassayJ．JChromatogrB20007391163．6Alvarez－LorenzoCConcheiroA．MolecularlyimprintedpolymersfordrugdeliveryJ．JChromatogrB20048041231．7LaiJPHeXWGuoHSetal．AreviewonmolecularimprintingtechniqueJ．ChinJAnalChem2001297836．8CuiASinghAKaplanDL．Enzyme－basedmolecularimprintingwithmetalsJ．Biomacromolecules2002361353．9ParmpiPKofinasP．BiomimeticglucoserecognitionusingmolecularlyimprintedpolymerhydrogelsJ．Biomaterials200425101969．10．J．2005365477．11C．J．AllenderC．RichardsonB．Woodhouseetal．PharmaceuticalapplicationsformolecularlyimprintedpolymersJ．InternationalJour-nalofPharmaceutics200019539．12．J．2002603385．13．J．2004195232．14EldridgeGRVervoortHCLeeCMetal．High－throughputmeth-odfortheproductionandanalysisoflargenaturalproductlibrariesfordrugdiscoveryJ．Ana1．Chem2002743963．15AbelUKochCSpeitlingMeta1．Modernmethodstoproducenatu-ral－productlibrariesJ．Curr．Opin．Chern．Biol200264453．16．1BJ．2005175606．17．J．－20002447．18．2J．200624101840．19．J．2007353132171．20．J．20023712930．21PersidisAris．BiochipsJ．NatureBiotechnol199816981．22．M．20006．23．J．20044142．24．J．2002233273．25LiXXueXLiPC．Real－timedetectionoftheearlyeventofcyto-toxicityofherbalingredientsonsingleleukemiacellsstudiedinami-crofluidicbiochipJ．IntegrBiolCamb20091190．26ChangSK．Biotechnology－updatesandnewdevelopmentsJ．Bi-omedEnvironSci2001141－232．27．J．20022112757．28．－M．20071．29BaoYGQiZFBaoL．CloningexpressionandbioinformaticanalysisofRv3871generelatedtoMycobacteriumtuberculosisvirulentproteinsecretionJ．NanFangYiKeDaXueXueBao200929122371．30PhilippeBernardThomasSciorBrunoDidiereta1．Ethnopharma-cologyandbioinformaticcombinationforleadsdiscoveryapplicationtophospholipaseA2inhibitorsJ．Phytochemistry200158865．31．J．20032810907．32BirnbaumerGMLieberzeitPARichterLeta1．Detectionofviruseswithmolecularlyimprintedpolymersintegratedonamicrofluidicbiochipusingcontact－lessdielectricmicrosensorsJ．LabChip20099243549．·3762·LISHIZHENMEDICINEANDMATERIAMEDICARESEARCH2010VOL．21NO．1020102110