pETDuet1载体基本信息

pETDuet1载体基本信息出品公司:EMDBiosciences(Novagen)别名:pETDuet1,pETDuet1,pETDuet-1质粒类型:大肠杆菌(Escherichiacoli)蛋白双表达载体，有两个多克隆位点表达水平:高克隆方法:多克隆位点，限制性内切酶载体大小:5420bpMCS15'测序引物及序列:pETUpstreamPrimer:5'-ATGCGTCCGGCGTAGA-3'MCS13'测序引物及序列:DuetDOWN1Primer:5'-GATTATGCGGCCGTGTACAA-3'MCS25'测序引物及序列:DuetUP2Primer:5'-TTGTACACGGCCGCATAATC-3'MCS23'测序引物及序列:T7t:5'GCTAGTTATTGCTCAGCGG3'载体标签:N-His,C-S载体抗性:氨苄备注:pETDuet-1是设计用来同时表达两个目的基因。pETDuet-1携带有pBR322质粒来源的ColE1复制子,lacI基因和氨苄抗性基因。产品目录号:71146-3稳定性:瞬时表达组成型:组成型病毒/非病毒:非病毒pETDuet1载体质粒图谱和多克隆位点信息pETDuet1载体简介pETDuet-1载体被设计用来在大肠杆菌(Escherichiacoli)中同时表达两个外源基因.载体含有两个多克隆位点，每一个多克隆位点是通过T7启动子/Lac操纵子和一个核糖体结合位点组成。pETDuet-1携带有pBR322质粒来源的ColE1复制子,lacI基因和氨苄抗性基因。本载体可以与pACYCDuet-1载体联合使用，在合适的宿主菌中，可以一起共表达至多4个目的基因。插入在pETDuet-1载体多克隆位点1的目的基因可以使用pETUpstreamPrimer(Cat.No.69214-3)和DuetDOWN1Primer(Cat.No.71179-3)进行测序，插入在多克隆位点2的目的基因可以通过DuetUP2Primer(Cat.No.71180-3)和T7TerminatorPrimer(Cat.No.69337-3)进行目的基因测序。pETDuet1载体序列LOCUSpETDuet-15420bpDNAcircular7-SEP-2008SOURCEORGANISMCOMMENTThisfileiscreatedbyVectorNTI|GenBankCOMMENTVNTAUTHORNAME|biofeng.com|COMMENTVNTOAUTHORAD1|NovagenInc.|COMMENTVNTREPLTYPE|PlasmidFEATURESLocation/Qualifierspromoter5404..5420/vntifkey=30/label=T7\promoter-1misc_feature1..1/vntifkey=21/label=T7\transcription\start-1misc_feature83..100/vntifkey=21/label=His-Tagmisc_feature70..168/vntifkey=21/label=MCS-1promoter214..230/vntifkey=30/label=T7\promoter-2misc_feature231..231/vntifkey=21/label=T7\transcription\start-2misc_feature297..438/vntifkey=21/label=MCS-2misc_feature366..410/vntifkey=21/label=S-Tagterminator462..509/vntifkey=43/label=T7\terminatoroperon3931..5013/vntifkey=103/label=lacI\operonrep_origin2737..2737/vntifkey=33/label=pBR322\originmisc_feature1119..1976/vntifkey=21/label=Amprep_origin547..994/vntifkey=33/label=f1\originBASECOUNT1274a1434c1400g1312tORIGIN1ggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaag61gagatataccatgggcagcagccatcaccatcatcaccacagccaggatccgaattcgag121ctcggcgcgcctgcaggtcgacaagcttgcggccgcataatgcttaagtcgaacagaaag181taatcgtattgtacacggccgcataatcgaaattaatacgactcactataggggaattgt241gagcggataacaattccccatcttagtatattagttaagtataagaaggagatatacata301tggcagatctcaattggatatcggccggccacgcgatcgctgacgtcggtaccctcgagt361ctggtaaagaaaccgctgctgcgaaatttgaacgccagcacatggactcgtctactagcg421cagcttaattaacctaggctgctgccaccgctgagcaataactagcataaccccttgggg481cctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggattggcga541atgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgt601gaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttct661cgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccg721atttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtag781tgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaa841tagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttga901tttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaa961atttaacgcgaattttaacaaaatattaacgtttacaatttctggcggcacgatggcatg1021agattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatca1081atctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggca1141cctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtag1201ataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagac1261ccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgc1321agaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagct1381agagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatc1441gtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaagg1501cgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatc1561gttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataat1621tctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaag1681tcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggat1741aataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcgggg1801cgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgca1861cccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacagga1921aggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactc1981ttcctttttcaatcatgattgaagcatttatcagggttattgtctcatgagcggatacat2041atttgaatgtatttagaaaaataaacaaataggtcatgaccaaaatcccttaacgtgagt2101tttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctt2161tttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggttt2221gtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgc2281agataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctg2341tagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcg2401ataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggt2461cgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaac2521tgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcgg2581acaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggg2641gaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgat2701ttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttt2761tacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctg2821attctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaa2881cgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttc2941tccttacgcatctgtgcggtatttcacaccgcatatatggtg