微生物发酵法生产1-3-丙二醇

200213sr:1,3l,3(PDO)—(),、、、。,PDO3:;;。PDO、,,PDO。1,3Producitonofl,3ProPylenediolVlaMicrobiologicalFerUOenattionMethodZhaoHongyingAbstraetPorduetionofl,3porpylenediol(l,3PDO)basedonrenewableresoucrestacrhviamieorbiologiealfer-mentationmethod15ehaareteirzedbyeasyoperation,modearteeraetioneonditions,lessbyPorduetandlesspollution.AtPersent,thereaerthreemethodstakingearbohydartedieretlyasrawmaterialforPordueingl,3PDOinodrertodeeerasetheeostofmieorbiologiealfemrentation:usingglueoseasauxiliayrsubstrate,adoPtingmixedstrainortwostePfermentationmethodandmakinggenetieengineeredstarin.Researehofsepaartingandpuirfyingl,3PDObasedontheooffomringae-etalformdihydorxyaleoholandethylal15earriedoutbytheDepartmentofChemiealEngineeirngofTsinghuaUnivesrity·Moerover,basedonglyeerineporduetionviatwostepfemrentationPatentteehnolo,anewporeesswhieheoupleanaeorbieearbohydrateeonsumptionproeess,thesecondstePofglyeerinefemrentation,andPDOanaeorbiefemrentationhasbeende-veloPed.Keywordsl,3PDO,mieorbiolofermentationmethod,glyeerine,3(l,3porpanediol,pDO)LJ,,、、、。PDO(PTT),PTT50(PET)、70(PBT),1998。PET、PBT,PTT、、、、、、,、、。PTTPDO,PTT。,DupontShellPDO,DegussaShen。、、、、、。,,PDO、。PDO—(),、、、,。(eBF),Dupont、Geneneor,、、PDO,、、、,PDO。`2002’3,l、mgerss1PDO1.1PDOPDO。,,(eb-siellapneumoniae)、(Cirorbae`erferud-11)、(Enreorbaeteralemearn)(Clos`riumbuytrieu。)Pno。leKbsiellaneumoniaepDO、,、。1。PDO:,,3,3PDOPDO;,,,。PDO,,。PDO,(NADH)。,,,3;;PDO,,NADHNAD`,;,,。1.2PDO。,PDO。,PDO55-73L。,PDO,,,。aSintAmansCOZ,,65L,1.21L·h,0.56mol/mol。Reimann,pH,70L,1.8-2.4L·h。^OPJN^OTPINAD】I=t`”\N~DHa.D^P、!/~^T”`”D^ōùùóōCZNDpZADP7D^PND^~、-ccoA、、,,.,2“)/+NAD2,3·’rInl,3W1。MenezllKebllapneumoniae0.lh’,PDO3.3-4.8『.Lh,PDO48L,0.63moFmol。。,、,Pfugacher、Riemann。34,PDO,1926.5L。,。1.3PDO,、,,PDO,。,PDO21,3200213:sr,,21,3PDO,、。。:PDo+:l,3,PDo,PDO,。1.4,,,,,PDO。PDO3:(l),PDO。H.BieblC.utrieumC.ferundii,,,PDOATP,0.gmol/mol。,,。(2),,PDOPDO,。Hag-nie5.eeisiaeC.freundiiK.pneumo-ina。,PDO4.789/L。,,。(3),。PDO,PDO,3:①PDOdhaBdhaT;②GPPI2/pDo;③dhaB、dhaT、GPPI/2,。DuPontGenecnor,,。,Tate&Lyle,10,20032004,Pno。1.5,PDO,。,,“”。、,2003。,PDO,,PDO,PDo。2,2010,PTT100ta/,,PDO。,、PDO,,lP〕O。、,。PDO,,。,,、,PDO,、PDO,,。,,,,,。(38)200213,1996n19931995“”。2000,,A。.,,。19948,19976。60.5%。。.(),“。”。19956,1998323,2000t,20002500,。90%。,。.,Mylar。,199512,51%,1996n,,。2000。.,66,99.5%,199511,19979。5t“,。.,。19984,1998514。50%,。,。..··,1999,,。,56%51%。:、、。、、,。,。...()(())(23)〔1〕Cornyieldsalower。tpolymered:DuPont,Tate&dGeneneorInternationalInjoinilystartup100,000lb/yrplanttoprodueel,3propanediolfromeominsteadoffromPetroleum.CemiealEngineeirng,2001,108(6):23(2〕BieblH.,Men:elK.,ZengA.P.,etal.Mieorbialporduetionofl,3propanediol.ApplMierobiolBioteehnol,1999,52:28997〔3〕SprenrG.A.,HammerB.A.,JohnsonE.A.,etal.AnaeorbiewthofE.eolion91”eorlbyimportinggenesOfthedharegulonofrmKlebsiellapneumoniae(J〕.GenearlMieorbiol,1989,135:12551262(4〕Laffend,LisaAnne,Nagaarjan,etal.Bioeonversionofaferment-ableearbonsoucretol,3PorPanediolbyasinglemieoroarsnism(p〕.US,6025184.2000〔5〕Nakamuar,CharlesE,etal.Mehtodofrtheporduetionofl,3-propanediolbyrecombinantmieoroanisms〔P〕.US,6013494.2000(6〕Haynie,ShaornL,Wa,er,。tal.porees。ofmakingl,3porpane-diolmeahtonhydartesusingmixedmieorbialeultuer〔P〕.US,5599689.1997BieblH.,Marten5.useofcosubates.Fermentationofglyeeorltol,3porpanediol:A1Mieorb1OIBiotechnol,1995,44:1519,,.1,3.(),2001,12:535、..J、es70.r`..、〔9〕,,,,.1,3.,2000,04:420422