病毒的检测方法

()():R141.04:A:1006-351X(2008)04-0553-03:050000:(DNARNA),,,,,,,,,,,,,,,,,1.,,[1],,,,2.,,,,,,,3.,,(),pH7.27.4(7),,,,,,[2]1.(completmentfixation,CF),,:;;,;,:,,,,2.,4,,(),50-100,:,,,-K,:,,,,,,,(-),,[3]3.(ELISA)1971Engvall11Perlmann(enzymelinkedimmunosorbentassay,ELISA)IgGELISA,,,,ELISAIgAIgGIgM(PCR):ELISAPCR,IgG(54%),,,IgM,(34%)4.(1FA),3552008164,,,,,,IFA,92%,CoxB100%,ECHO94%,IFA,,PCR[4]5.(E-DBA)80Huel[5],ELISA,97.14%90%ELISA,,ELISA1.,Hall1961,,()(probe),,DNARNA:2.(PCR)(PCR),1985,[6]2.1PCRKorana1971,1985PE-CetusMullisTaqDNA,DNADNAPCR---.:,;;;:,;,;2.2PCRPCR(realtimequantitativePCR,RT-PCR)PCR(),PCR,(thresholdcycle,Ct)Ct,Ct,,,Ct(),Ct,PCRPCR,,.Boeckh[7],DNAHCMVMengoli[8]PCRpp65pp67RNAHCMV,3HCMVStocher[9]HCMV5DNAPCR,2.3PCR(RT-PCR)RT-PCR(PCR)FIT-PCR,RNADNA,PCRDNABoriskin[10]RT-PCRHCMVRNAUL21.5,DNA14w,,2.4PCR(nPCR)PCR(PCR),()PCRPCRPCR(PCR)PCR,PCRPCR,,:,,Distefano[11]PCRnPCR,GbDNA100%,LA54%61%,nPCR97%1.(nOWCytometry,FCM)FCM,,,Essa[12]FCMHCMVpp65,HC2MV,pp65IHCMV2.DNA,DNA4552008164Anthony4,PCR23sDNA,:,,;,,,;,,;:,,,,,,3.(Proteinmicroarray),,,,,Mezza2soma[13],,,,,,,,,,,,120062-()20053GravesPM,TheroleOfenterovirusinfectionsinthedevelopmentOfIDDM.Diabetes,1997;46:1614RigonanAS,MannL,ChonmaitreeT.UseOfmonoclonalantibodiestoidentifyserotypesOfenterovirusisolates.JClinMicrobio1.1998:36(7):1877-18815,.B.,1992;20(2):1276BaumforthKR,NelsonPN,DigbyJE,etal.Demystifiedthepoly2merasechainreaction.Molpathol,1999,52:1-10.7BoeckhM,HuangM,FerrenbergJ,etal.OptimizationOfquantita2tivedetectionOfcytomegalovirusDNAinplasmabyreal2timePCR.ClinMicrobiol,2004,42(3):1142-1148.8MengoliC,CusinatoR,BiasoloMA,etal.AssessmentOfCMVloadinsolidorgantransplantrecipientSbypp65antigenemiaandre2al2timequantitativeDNAPCRassay:correlationwithpp67RNAdetection.JMedVirol,2004,74(1):78-84.9StocherM,LebV,BozicM,etal.ParalleldetectionoffivehumanherpesvirusDNAsbyasetofrealtimepolymerasechainreactions.inasinglerun.JClinVirol,2003,26(1):85-9310BoriskinYS,FullerK,PowlesRL,etal.Earlydetectionofcyto2megalvirus(CMV)infectioninbonemarrowtransplantpatientsbyreversetranscriptionPCRforCMVsplicedlategeneUL21.5;atwositeevaluation[J].JClinVirol2002,24(122):13-2311DistefanoAL,AlonsoA,MartinF,etal.Humancytomegalovirus:detectionofcongenitalandperinatalinfectioninAregntin[J].BWCPediatr,2004,4(1):11.12EssaS.PacsaAS.TheuseofflowcytometryforthedetectionofCMVspectificantigen(pp65)inleukocytesofkidneyrecipients[J].ClinTransplant,2000,14(2):14713MezzasomaL,BacareseHamiltonT.Antigenmicroarraysforsero2diangnosisofinfectiousdiseases[J].ClinChem,2002,48(1):121(2008-03-16),,,,:/50,,,E-mail20085.8,139.2(:80-258),:10,128,;:100069;:010-63052088,010-83525550;:010-63052088;E-mail:[e-mail]5552008164