乳酸菌胞外多糖的结构及功能特性研究进展-田政

43Vol.4No.320136JournalofFoodSafetyandQualityJun.,2013基金项目：北京市教育委员会项目(19000550012)Fund:SupportedbyBeijingMunicipalCommissionofEducation(19000550012)*通讯作者:,,E-mail:[e-mail]*Correspondingauthor:YANGZhen-Nai,Professor,BeijingTechnologyandBusinessUniversity,No.11,FuchengRoad,HaidianDistrict,Beijing100048,China.E-mail:[e-mail]田政1,王辑2,郑喆1,杨贞耐1*(1.,100048;2.,130025)摘要:,,,关键词:;;;ResearchadvancesonstructureandfunctionofexopolysaccharidesproducedbylacticacidbacteriaTIANZheng1,WANGJi2,ZHENGZhe1,YANGZhen-Nai1*(1.SchoolofFoodScience,BeijingTechnologyandBusinessUniversity,Beijing100048,China;2.CollegeofBiologicalandAgriculturalEngineering,JilinUniversity,Changchun130025,China)ABSTRACT:Theexopolysaccharides(EPSs)producedbylacticacidbacteriawerenaturalbiologicalpoly-merswithmanyspecialfunctionssuchasimprovementoftexturalcharacteristicsoffermenteddairyproductsandbeneficialhealtheffectsforhumanbeings.EPSsoflacticbacteriahadbeenextensivelyandthoroughlystudiedwithrespectstothestructureandfunction,aswellastheirrelations.ThispaperreviewedtheresearchadvancesontheEPStypes,chemicalcomposition,structureandfunction,inordertoprovidereferencesforfurtherresearchanddevelopmentoffunctionalfoodsrelatedtotheEPSsoflacticacidbacteria.KEYWORDS:lacticacidbacteria;exopolysaccharides;structure;function1引言(exopolysaccharide,EPS)[1]2040,EPSEPS,EPS[2]Mende[3]EPS,,EPS,-Zhang[4]ST1EPS1%(w/v),Purwandari[5],EPS,7844Girard[6]EPS,[3],EPSZhang[7]C88EPS,,EPSWu[8]EPSUchida[9]EPS,EPSEPS[10],EPSEPS2乳酸菌EPS的分类、化学组成和结构2.1乳酸菌EPS的分类EPS,EPS(capsularpolysaccharides,CPS),EPS(slimepolysaccharide,SPS),EPSSPS,CPSSPS[11],EPS:(homopolysaccharides,HoPS),,[12](heteropolysaccharides,HePS),,3~8[13]-,,[14]2.2乳酸菌EPS的化学组成和结构EPS,—[15],[16]HoPS4.0×104∼6.0×106[17],:(1)α-D-,α-1,6,α-1,3α-1,2α-1,4,Lb.Parabuchneri33[18]S.sobrinus[18]Lb.reuteri180[19]Lb.reuteri35-5[20]EPS;α-1,3,α-1,3,Lb.reuteriML1[21]LeuconostocmesenteroidesNRRLB-523[22]EPS;α-1,4,α-1,6,Lb.reuteriLBBIO[123]EPS;α-1,6α-1,3,α-1,3,S.gordoniiCH1[24]EPS(2)β-D-,β-1,3,β-1,2,Pediococcusdamnosus2.6[24]Lb.brevisG-77[25]EPS(3)β-D-,β-2,6,β-2,1,Lb.frumenti[26]EPS;β-2,1,β-2,6,S.mutansIngbrittA[27]EPS(4),,HePS1046.0×106[28],HoPSHePS(C2,C3,C4C6)HePS,HePS,D-D-L-,,D-,D-,L-,D-D-EPSN-N-,α-β-1990Doco[29]S.thermophilusHePS,HePS,1HePSHePS,α-β-,D-3,:785表1部分乳酸菌HePS单糖组成Table1MonosaccharidecompositionofsomeheteropolysaccharidesproducedbylacticacidbacteriaLactobacillusFermentumTDS030603:3:1[30]Lactobacilluskefiranofaciens:2:4[31]LactobacillusrhamnosusKL37B:3:6[32]lactisIPLA-R1::1:2:3[33]Lactobacillusjohnsonii142:1:4[34]LactobacillushelveticusLb161:5:2[35]LactobacillushelveticusK16:4:2[36]Escherichiacoli180/C3::1:2:1[37]LactobacillusrhamnosusGG::1:4:1[38]StreptococcusthermophilusTHS:3:2[39]Lactobacillusdelbrueckiissp.bulgaricusLBB.B26:2:3[40]Lactobacillusdelbrueckiissp.bulgaricusLBB.B332::1:2:2[41]Lactobacillusacidophilus5e2:4:3[42]LactobacilluspentosusLPS26:3:2[43]D-L-D-D-(D-Galp)D-(D-Galf),D-L-D-(D-Glcp)L-(L-Rhap),HePSN-(GlcpNAc),Lb.acidophilus5e2[42]Lb.pentosusLPS26[43]EPS,,EPS,,EPS,3乳酸菌EPS功能特性(generallyrecognizedassafe,GRAS),,,EPSEPS,,EPSEPS,,3.1乳酸菌EPS的物理学特性,,[44],EPS[45]EPS,Ayala-Hernandez[46]Lactococcuslactissubsp.cremorisJFR1EPS,,pH5.66.7,,4℃6h,EPS,,pHHassan[47]CPS,SPSCPS,,EPS7864[48]Purwandari[5]EPSS.thermophilus,EPSRawson[49]SPS,Perry[50]EPSMozzarella,EPS,EPS,pHEPSEPS,3.2乳酸菌EPS的生理学特性3.2.1乳酸菌EPS的抗肿瘤作用1982Shiomi[51]EPS,EPSEPS[52]EPSKL1,EPSHCT-8CCK-8,EPSHCT-8,-AnnexinV-FITC,EPS,EPSHCT-8,HCT-8[53]70810EPS-1EPS-2HT-29,EPS,5-Fu50μg/mL,68.51%EPS-2EPS-1,Haroun[54]Lb.plantarumNRRLB-4496β-(1→4)β-(1→6),(CACOHELAHCT116HEPG2MCF7HEP2),9.0715.817.619.924.234.7μg/mL,88.9Liu[55]Lb.casei01EPSHT-29,4074--1-3.2.2乳酸菌EPS的抗氧化作用,,EPS[56]KW3EPS,,EPS200μg/mL,DPPH37.53%,FRAP(600.16±15.23)FeSO4·7H2Oμmol/LEPSMDA,SODGSH-Px[57]H9EPS,EPS200mg/L,DPPH(55.3±1.5)%(68.7±1.1)%(70.8±0.6)%[58]EPS,EPS,Kanmani[59]S.phocaePI80ESPPan[60]Lactococcuslactissubsp.lactis126.9×105Da,EPS,,3.2.3乳酸菌EPS的免疫调节作用,EPS[61](Se-EPS),Se-EPS,NOTNF-α(NO,;NF-α,),Se-EPS501000μg/mLSe-EPS,Kang[62]Lb.paracaseiKB283,:787EPSNF-αIL-6IL-12Ruas-Madiedo[63]Bifidobacteriumanimalisssp.lactisA1IPLA-R1BifidobacteriumlongumNB667Lb.rhamnosusGGEPS1μg/mL,Wu[8]BifidobacteriumlongumBCRC14634EPS80μg/mL73.2.4乳酸菌EPS的益生特性EPS,,,Looijesteijn[64]Lactissubsp.cremorisNZ4010,EPS,100,()EPS,,EPSEPS,CPSEPS,,EPS,3.2.5其他EPS,[65]Buijssen[66]SPSCerning[67],Trevors[68],EPS,EPS[69-71]4结语EPS,,EPS,:(1),(2)EPS,EPS,EPS,EPS,参考文献[1]SutherlandIW.Bacterialexopolysaccharides[J].AdvMicrobPhysiol,1972,8:142–213.[2]RicciardiA,ClementiF.Exopolysaccharidefromlacticacidbacteria:Structure,productionandtechnologicalapplications[J].ItalJFoodSci,2000,1:23–45.[3]MendeS,PeterM,BartelsK,etal.Additionofpurifiedexopo-lysaccharideisolatesfromS.thermophilustomilkandtheirim-pactontherheologyofacidgels[J].FoodHydroeolloids,2013,32:178–185.[4]ZhangTH,ZhangCH,LiSH,etal.Growthandexopolysaccha-rideproductionbyStreptococcusthermophilusST1inskimmilk[J].BrazJMicrobiol,2011,42:1470–1478.[5]PurwandariU,ShahNP,VasiljevicT.Effectsofexopolysaccha-ri