加强临床流式细胞免疫表型分析的质量控制

:100034,(FCM),FCM,,;;/,;;THLA2B27,,(MoAb),,,6h;,,,EDTA2K2012m,011%,,,,,(medimachine)()(BDFACSLysingsolution),MoAb,()MoAb,,WBC1000/l,200l4ml(FACSLysingSolution)MoAb20l(BDMoAb);WBC100010000/l,100l2mlMoAb20l;WBC1000020000/l,50l1mlMoAb20l;WBC20000/l,100010000/l,100l1mlMoAb20l11:MoAbMoAb,MoAb,MoAb,5%,MoAb,,1,,21:,520031261ChinJLabMed,January2003,Vol26,No11©1995-2004TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.MoAbMoAbMoAb31:MoAb,20(BDCDChex)MoAb,MoAb41:MoAb3:(invitrodiagnosis,IVD)(analyte2specificreagents,ASR)(onlyresearch)MoAb,IVDASR,,/HLA2B27,,,;,MoAb,CD55CD59,/MoAb,MoAb,,MoAb,,MoAb,,MoAb,,1,(controls),(positivecontrol)(negativecontrol)(normalcontrol)(isotypecontrol)(blankcontrol)11:,CD3MoAbT60%,,MoAb21:MoAb,,,IgG12FITCIgG2a2PEFC,MoAbF:P(),,MoAb,()31:,PBSHEPESMoAb,,,41:()MoAb(CD192FITC),,,,,,CD4195%,(MFI)510,,51:,MoAb(I),CD41CD61,CD41,,620031261ChinJLabMed,January2003,Vol26,No11©1995-2004TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.,ICD415%61:(),,,(MPO),,(),(),MPO,(1)(farwardscatter,FSC)(sidescatter,SSC);(2);(3);(4);(5)3(BDFACSCalibur)(BDFACSComp)(BDCaliBRITE),,,(multi2levelfluorescencebeads),(PMT)(MFI),MFI,r20195,(cross2instrumentsampletest),5,,,,,,PMT,()(dotplot),10000,2000100002000,,2000(gating)(),,()SSC/CD45(SSC),,,/,,,,,,//(standardoperatingprocedures,SOPs)(oualitycontrol,QC),,,(:2002211207)(:)20032003:1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:720031261ChinJLabMed,January2003,Vol26,No11©1995-2004TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.